Abstract
Acid soils whose pH is low enough for A1 to become soluble are found throughout the world and greatly limit growth of Al‐sensitive crops like alfalfa, Medicago sativa L. Alfalfa germplasms were selected to overcome these conditions and are now available for testing. The objective of this study was to test performance of an acid‐tolerant germplasm (AT), and an acid‐sensitive check germplasm (AS), in acid, Al‐toxic cell culture conditions. Both germplasms were tested for growth as callus in normal Biaydes medium, a Blaydes medium modified by lowering pH and levels of Ca and P, and on the same modified medium but with a toxic Al level. The modified Blaydes medium depressed callas growth of both germplasms relative to their growth on normal Blaydes. This depression was equal for both germplasms of the first 6 wk of culture, but by the 8th wk, the AT germplasm outperformed the AS germplasm. The addition of Al further decreased growth of AS callus but not AT callus. This suggests that plants from the AT germplasm rapidly express Al tolerance at the tissue level. With increasing time in culture, the AT germplasm also expresses acid tolerance in culture. The differences in callus growth shown by genotypes within germplasms indicates the cell culture procedure can be used to screen genotypes for acid or Al tolerance. Significant genotypic variability for acid, Al tolerance is still present within the germplasm, suggesting that continued progress from selection should still be possible.
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Claims 9
Claim · model-written summary
Modified Blaydes medium depressed growth of both alfalfa germplasms.
- Taxon
- alfalfa
Medicago sativae1 - Treatment
- modified Blaydes mediume0
- Outcome
- callas growthe0
- Direction
- depressede0
- Comparator
- normal Blaydese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The modified Blaydes medium depressed callas growth of both germplasms relative to their growth on normal Blaydes.”
e1“alfalfa, Medicago sativa L.”
Claim · model-written summary
By week 8, acid-tolerant alfalfa germplasm outperformed the sensitive germplasm.
- Taxon
- alfalfaMedicago sativae1
- Material
- AT germplasme0
- Outcome
- outperformede0
- Direction
- outperformede0
- Qualifier
- 8th wke0
- Comparator
- AS germplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by the 8th wk, the AT germplasm outperformed the AS germplasm”
e1“alfalfa, Medicago sativa L.”
Claim · model-written summary
Aluminum further decreased sensitive alfalfa callus growth.
- Taxon
- alfalfaMedicago sativae1
- Material
- AS calluse0
- Treatment
- addition of Ale0
- Outcome
- growth of AS calluse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of Al further decreased growth of AS callus but not AT callus.”
e1“alfalfa, Medicago sativa L.”
Claim · model-written summary
Aluminum did not further decrease tolerant alfalfa callus growth.
- Taxon
- alfalfaMedicago sativae1
- Material
- AT calluse0
- Treatment
- addition of Ale0
- Outcome
- AT calluse0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of Al further decreased growth of AS callus but not AT callus.”
e1“alfalfa, Medicago sativa L.”
Claim · model-written summary
Tolerant alfalfa germplasm expressed aluminum tolerance at tissue level.
- Taxon
- alfalfaMedicago sativae1
- Material
- AT germplasme0
- Outcome
- Al tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants from the AT germplasm rapidly express Al tolerance at the tissue level”
e1“alfalfa, Medicago sativa L.”
Claim · model-written summary
Tolerant alfalfa germplasm expressed acid tolerance with increasing time in culture.
- Taxon
- alfalfaMedicago sativae1
- Material
- AT germplasme0
- Outcome
- acid tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With increasing time in culture, the AT germplasm also expresses acid tolerance in culture.”
e1“alfalfa, Medicago sativa L.”
Claim · model-written summary
Callus growth differences indicate cell culture can screen alfalfa genotypes for acid or aluminum tolerance.
- Taxon
- alfalfaMedicago sativae1
- Outcome
- screen genotypes for acid or Al tolerancee0
- Comparator
- genotypes within germplasmse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differences in callus growth shown by genotypes within germplasms indicates the cell culture procedure can be used to screen genotypes for acid or Al tolerance”
e1“alfalfa, Medicago sativa L.”
Claim · model-written summary
Significant genotypic variability for acid and aluminum tolerance remains within alfalfa germplasm.
- Taxon
- alfalfaMedicago sativae1
- Material
- germplasme0
- Outcome
- genotypic variability for acid, Al tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant genotypic variability for acid, Al tolerance is still present within the germplasm”
e1“alfalfa, Medicago sativa L.”
Claim · model-written summary
The authors suggest further selection for alfalfa tolerance remains possible.
- Taxon
- alfalfaMedicago sativae1
- Outcome
- continued progress from selectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“continued progress from selection should still be possible”
e1“alfalfa, Medicago sativa L.”
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See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.