Abstract
The effects of various combinations of heat shock temperature and duration, medium renovation and cold shock pretreatment on the embryogenesis of isolated microspores were studied in two F1 hybrids of winter cauliflower (Brassica oleracea var. botrytis). The results indicated that the optimal combination of heat shock temperature and duration was 32℃ and 24 h. A decrease in sucrose concentration from 17% to 10% in liquid NLN medium following the first 24 h incubation at 32℃ significantly enhanced microspore embryogenesis. Both medium renovation and cold shock pretreatment markedly improved the quality of normal embryos and reduced the number of callus-like embryos. After 24 h induction at 32℃ and continuous culture at 24℃ in darkness for 30 d, isolated microspores produced normal embryos, which were then transferred to solid MS medium for embryo germination and root development. After two times of subculture, large amounts of normal regenerated plants were produced from generated shoots, and these regenerated plants were mainly spontaneous doubled haploids.
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Claims 9
Claim · model-written summary
The optimal heat shock for cauliflower microspore embryogenesis was 32℃ for 24 h.
- Taxon
- winter cauliflower (Brassica oleracea var. botrytis)
Brassica oleracea var. botrytise1 - Treatment
- heat shocke0
- Outcome
- optimal combination of heat shock temperature and duratione0
- Value
- 32℃ and 24 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“optimal combination of heat shock temperature and duration was 32℃ and 24 h”
e1“winter cauliflower (Brassica oleracea var. botrytis)”
Claim · model-written summary
Reducing sucrose in liquid NLN medium from 17% to 10% after 24 h at 32℃ significantly enhanced microspore embryogenesis.
- Taxon
- winter cauliflower (Brassica oleracea var. botrytis)Brassica oleracea var. botrytise1
- Treatment
- decrease in sucrose concentration from 17% to 10% in liquid NLN mediume0
- Outcome
- microspore embryogenesise0
- Direction
- enhancede0
- Qualifier
- following the first 24 h incubation at 32℃e0
- Comparator
- 17%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A decrease in sucrose concentration from 17% to 10% in liquid NLN medium following the first 24 h incubation at 32℃ significantly enhanced microspore embryogenesis.”
e1“winter cauliflower (Brassica oleracea var. botrytis)”
Claim · model-written summary
Medium renovation improved the quality of normal embryos.
- Taxon
- winter cauliflower (Brassica oleracea var. botrytis)Brassica oleracea var. botrytise1
- Treatment
- medium renovatione0
- Outcome
- quality of normal embryose0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both medium renovation and cold shock pretreatment markedly improved the quality of normal embryos and reduced the number of callus-like embryos”
e1“winter cauliflower (Brassica oleracea var. botrytis)”
Claim · model-written summary
Cold shock pretreatment improved the quality of normal embryos.
- Taxon
- winter cauliflower (Brassica oleracea var. botrytis)Brassica oleracea var. botrytise1
- Treatment
- cold shock pretreatmente0
- Outcome
- quality of normal embryose0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both medium renovation and cold shock pretreatment markedly improved the quality of normal embryos and reduced the number of callus-like embryos”
e1“winter cauliflower (Brassica oleracea var. botrytis)”
Claim · model-written summary
Medium renovation reduced the number of callus-like embryos.
- Taxon
- winter cauliflower (Brassica oleracea var. botrytis)Brassica oleracea var. botrytise1
- Treatment
- medium renovatione0
- Outcome
- number of callus-like embryose0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both medium renovation and cold shock pretreatment markedly improved the quality of normal embryos and reduced the number of callus-like embryos”
e1“winter cauliflower (Brassica oleracea var. botrytis)”
Claim · model-written summary
Cold shock pretreatment reduced the number of callus-like embryos.
- Taxon
- winter cauliflower (Brassica oleracea var. botrytis)Brassica oleracea var. botrytise1
- Treatment
- cold shock pretreatmente0
- Outcome
- number of callus-like embryose0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both medium renovation and cold shock pretreatment markedly improved the quality of normal embryos and reduced the number of callus-like embryos”
e1“winter cauliflower (Brassica oleracea var. botrytis)”
Claim · model-written summary
The specified 32℃ induction followed by 24℃ dark culture produced normal embryos.
- Taxon
- winter cauliflower (Brassica oleracea var. botrytis)Brassica oleracea var. botrytise1
- Treatment
- 24 h induction at 32℃ and continuous culture at 24℃ in darkness for 30 de0
- Outcome
- normal embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 24 h induction at 32℃ and continuous culture at 24℃ in darkness for 30 d, isolated microspores produced normal embryos”
e1“winter cauliflower (Brassica oleracea var. botrytis)”
Claim · model-written summary
After two subcultures, many normal regenerated plants were produced.
- Taxon
- winter cauliflower (Brassica oleracea var. botrytis)Brassica oleracea var. botrytise1
- Treatment
- two times of subculturee0
- Outcome
- large amounts of normal regenerated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After two times of subculture, large amounts of normal regenerated plants were produced from generated shoots”
e1“winter cauliflower (Brassica oleracea var. botrytis)”
Claim · model-written summary
The regenerated plants were mainly spontaneous doubled haploids.
- Taxon
- winter cauliflower (Brassica oleracea var. botrytis)Brassica oleracea var. botrytise1
- Outcome
- spontaneous doubled haploidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these regenerated plants were mainly spontaneous doubled haploids”
e1“winter cauliflower (Brassica oleracea var. botrytis)”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.