Abstract
Hybrids of cauliflower are in high demand world over due to their high yield potential, earliness, better quality, better resistance to biotic and abiotic stresses. Conventionally, hybrids are developed from the intercrossing of two diverse inbred parental lines which are developed through continuous inbreeding for 8-10 generations and still don't attain complete homozygosity. Doubled haploid technology on the other hand generate completely homozygous inbred lines in a single step. Therefore, a study was undertaken at Punjab Agricultural University, Ludhiana, to develop a protocol for the development of doubled haploid lines in cauliflower. The anthers were excised from the flower buds of different genotypes viz. Jyoti, Pusa Sharad, Kartiki, CAUMH-2, CAUMH-10, LS-2, LS-3, and LS-5 followed by their culture on five different callus induction media compositions. Genotypes differed significantly in the ability to induce callus which was maximum in Jyoti followed by LS-2. Different media compositions also varied significantly in callus induction efficiency which was maximum on MS media+1.5 mg/L 2,4-D +1.0 mg/L NAA. Maximum shoot regeneration was recorded in genotype Kartiki followed by LS-2 when cultured on MS media+3.0 mg/L BAP+2.0 mg/L Kin. The regenerated shoots thus obtained were rooted on ½ MS media +1.0 mg/L IBA. Ploidy analysis of root tips revealed that 22.2% of the regenerated plantlets were haploids, 27.8% were spontaneous doubled haploids, 16.7% were tetraploids and remaining 33.3% were mixoploids.
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Claims 7
Claim · model-written summary
Jyoti had the greatest callus induction, followed by LS-2.
- Taxon
- cauliflower
Brassica oleracea var. botrytist0 - Material
- Jyotie0
- Outcome
- ability to induce calluse0
- Direction
- maximume0
- Comparator
- LS-2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cauliflower”
e0“Genotypes differed significantly in the ability to induce callus which was maximum in Jyoti followed by LS-2.”
Claim · model-written summary
Callus induction efficiency was highest on MS media with 2,4-D and NAA.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Treatment
- MS media+1.5 mg/L 2,4-D +1.0 mg/L NAAe0
- Outcome
- callus induction efficiencye0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cauliflower”
e0“Different media compositions also varied significantly in callus induction efficiency which was maximum on MS media+1.5 mg/L 2,4-D +1.0 mg/L NAA.”
Claim · model-written summary
Kartiki had the highest shoot regeneration, followed by LS-2, on the stated medium.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Material
- Kartikie0
- Treatment
- MS media+3.0 mg/L BAP+2.0 mg/L Kine0
- Outcome
- shoot regeneratione0
- Direction
- Maximume0
- Comparator
- LS-2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cauliflower”
e0“Maximum shoot regeneration was recorded in genotype Kartiki followed by LS-2 when cultured on MS media+3.0 mg/L BAP+2.0 mg/L Kin.”
Claim · model-written summary
Haploids constituted 22.2% of regenerated plantlets.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Outcome
- regenerated plantlets were haploidse0
- Value
- 22.2%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cauliflower”
e0“22.2% of the regenerated plantlets were haploids”
Claim · model-written summary
Spontaneous doubled haploids constituted 27.8% of regenerated plantlets.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Outcome
- spontaneous doubled haploidse0
- Value
- 27.8%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cauliflower”
e0“27.8% were spontaneous doubled haploids”
Claim · model-written summary
Tetraploids constituted 16.7% of regenerated plantlets.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Outcome
- tetraploidse0
- Value
- 16.7%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cauliflower”
e0“16.7% were tetraploids”
Claim · model-written summary
Mixoploids constituted 33.3% of regenerated plantlets.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Outcome
- mixoploidse0
- Value
- 33.3%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cauliflower”
e0“remaining 33.3% were mixoploids”
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