Paper · 2007

Cloning of Capsicum annuum Antifungal Protein cDNA and Preliminary Analysis of Its Expression

Lei Cao

10
claims
19
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

Plants antimicrobial proteins(nsLTPS),is very important in plant disease resistance.In the study,a cDNA positive clone of Capsicum annuum antifungal protein was isolated from a cDNA library constructed from leaves challenged with phytophthora.The positive cDNA clone turned out to be 646 bps in length,harboring an open reading frame of 85 amino acids,which shared different degree of homology with chitinase or antimicrobial proteins from other plants,so the positive was named CansLTPS(Plants Antimicrobial Proteins of Capsicum annuum).The result of cDNA-AFLP analysis indicated that expression of CansLTPS was induced by UV-B illumination and phytophthora infection,and also by the treatment of exogenous phytohormones ABA,ETH and SA,but not by MeJA.The results indicated that CansLTPS might reply biotic and the abiotic stresses by the signal pathway mediaed by ABA,salicylic acid and ethylene.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher.

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

A Capsicum annuum antifungal protein cDNA clone was isolated.

Taxon
Capsicum annuumCapsicum annuume0
Gene
Capsicum annuum antifungal proteine0
Outcome
cDNA positive clonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a cDNA positive clone of Capsicum annuum antifungal protein was isolated”

✓ located verbatim in the source · abstract, characters 97–169 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The clone was 646 bps long.

Taxon
Capsicum annuumCapsicum annuume1
Outcome
cDNA clonee0
Value
646 bpse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The positive cDNA clone turned out to be 646 bps in length”

✓ located verbatim in the source · abstract, characters 243–301 · show in text ↑
e1

“Capsicum annuum antifungal protein”

✓ located verbatim in the source · abstract, characters 122–156 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Its open reading frame encoded 85 amino acids.

Taxon
Capsicum annuumCapsicum annuume1
Outcome
open reading framee0
Value
85 amino acidse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“harboring an open reading frame of 85 amino acids”

✓ located verbatim in the source · abstract, characters 302–351 · show in text ↑
e1

“Capsicum annuum antifungal protein”

✓ located verbatim in the source · abstract, characters 122–156 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The clone shared homology with chitinase or antimicrobial proteins from other plants.

Taxon
Capsicum annuumCapsicum annuume1
Outcome
homology with chitinase or antimicrobial proteinse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“shared different degree of homology with chitinase or antimicrobial proteins from other plants”

✓ located verbatim in the source · abstract, characters 358–452 · show in text ↑
e1

“Capsicum annuum antifungal protein”

✓ located verbatim in the source · abstract, characters 122–156 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CansLTPS expression was induced by UV-B illumination.

Taxon
Capsicum annuumCapsicum annuume1
Treatment
UV-B illuminatione0
Gene
CansLTPSe0
Outcome
expression of CansLTPSe0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“expression of CansLTPS was induced by UV-B illumination and phytophthora infection,and also by the treatment of exogenous phytohormones ABA,ETH and SA,but not by MeJA”

✓ located verbatim in the source · abstract, characters 586–752 · show in text ↑
e1

“Capsicum annuum antifungal protein”

✓ located verbatim in the source · abstract, characters 122–156 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CansLTPS expression was induced by phytophthora infection.

Taxon
Capsicum annuumCapsicum annuume1
Treatment
phytophthora infectione0
Gene
CansLTPSe0
Outcome
expression of CansLTPSe0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“expression of CansLTPS was induced by UV-B illumination and phytophthora infection,and also by the treatment of exogenous phytohormones ABA,ETH and SA,but not by MeJA”

✓ located verbatim in the source · abstract, characters 586–752 · show in text ↑
e1

“Capsicum annuum antifungal protein”

✓ located verbatim in the source · abstract, characters 122–156 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CansLTPS expression was induced by ABA.

Taxon
Capsicum annuumCapsicum annuume1
Treatment
ABAe0
Gene
CansLTPSe0
Outcome
expression of CansLTPSe0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“expression of CansLTPS was induced by UV-B illumination and phytophthora infection,and also by the treatment of exogenous phytohormones ABA,ETH and SA,but not by MeJA”

✓ located verbatim in the source · abstract, characters 586–752 · show in text ↑
e1

“Capsicum annuum antifungal protein”

✓ located verbatim in the source · abstract, characters 122–156 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CansLTPS expression was induced by ETH.

Taxon
Capsicum annuumCapsicum annuume1
Treatment
ETHe0
Gene
CansLTPSe0
Outcome
expression of CansLTPSe0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“expression of CansLTPS was induced by UV-B illumination and phytophthora infection,and also by the treatment of exogenous phytohormones ABA,ETH and SA,but not by MeJA”

✓ located verbatim in the source · abstract, characters 586–752 · show in text ↑
e1

“Capsicum annuum antifungal protein”

✓ located verbatim in the source · abstract, characters 122–156 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

CansLTPS expression was induced by SA.

Taxon
Capsicum annuumCapsicum annuume1
Treatment
SAe0
Gene
CansLTPSe0
Outcome
expression of CansLTPSe0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“expression of CansLTPS was induced by UV-B illumination and phytophthora infection,and also by the treatment of exogenous phytohormones ABA,ETH and SA,but not by MeJA”

✓ located verbatim in the source · abstract, characters 586–752 · show in text ↑
e1

“Capsicum annuum antifungal protein”

✓ located verbatim in the source · abstract, characters 122–156 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

MeJA did not induce CansLTPS expression.

Taxon
Capsicum annuumCapsicum annuume1
Treatment
MeJAe0
Gene
CansLTPSe0
Outcome
expression of CansLTPSe0
Direction
note0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“expression of CansLTPS was induced by UV-B illumination and phytophthora infection,and also by the treatment of exogenous phytohormones ABA,ETH and SA,but not by MeJA”

✓ located verbatim in the source · abstract, characters 586–752 · show in text ↑
e1

“Capsicum annuum antifungal protein”

✓ located verbatim in the source · abstract, characters 122–156 · show in text ↑

salicylic acid · molecular: expression · Capsicum annuum (sweet pepper)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
See all 3 papers →

(-)-methyl jasmonate · molecular: expression · Capsicum annuum (sweet pepper)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. no effect
See all 3 papers →

(+)-abscisic acid · molecular: expression · Capsicum annuum (sweet pepper)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

uv · molecular: expression · Capsicum annuum (sweet pepper)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive
See all 4 papers →

light · molecular: expression · Capsicum annuum (sweet pepper)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

infection · molecular: expression · Capsicum annuum (sweet pepper)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.