Abstract
NAC (NAM, ATAF1/2 and CUC2) proteins are plant-specific transcription factors (TFs) that are important in plant abiotic stress responses. However, knowledge of the functional roles that NACs play in abiotic stress tolerance is limited in pepper. In this study, we isolated a NAC TF gene, CaNAC035, from Capsicum annuum and showed that the protein is localized to the nucleus and functions as a transcriptional activator. CaNAC035 expression is induced by low and high temperatures, osmotic stres, ssalt, game archives (GA), methyl-jasmonic acid (MeJA) , salicylic acid (SA), and abscisic acid (ABA). To understand the function of CaNAC035 in the abiotic stress response, we used virus-induced gene silencing to reduce CaNAC035 expression, and overexpressed CaNAC035 in transgenic Arabidopsis plants. The results showed that pepper seedlings in which CaNAC035 was down-regulated showed more damage than WT after cold, NaCl, and mannitol treatments, and increased electrolyte leakage and higher levels of malondialdehyde (MDA), H2O2 and super oxide radical after cold treatment. CaNAC035-silenced seedlings had lower chlorophyll contents, and CaNAC035-overexpressing Arabidopsis plants had higher germination rates and fresh weights after mannitol and NaCl treatments. We also isolated 18 proteins that participate in processes such as the stress response, stress resistance, and photosynthesis, that potentially interact with CaNAC035. Our results suggest that CaNAC035 is a positive regulator of abiotic stress tolerance in pepper that acts through multiple pathways.
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Claims 10
Claim · model-written summary
CaNAC035 was isolated from Capsicum annuum.
- Taxon
- Capsicum annuum
Capsicum annuume0 - Gene
- CaNAC035e0
- Outcome
- isolated a NAC TF genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated a NAC TF gene, CaNAC035, from Capsicum annuum”
Claim · model-written summary
CaNAC035 protein localized to the nucleus.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Gene
- CaNAC035t0
- Outcome
- localized to the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the protein is localized to the nucleus”
t0“Molecular and Functional Characterization of CaNAC035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)”
Claim · model-written summary
CaNAC035 functioned as a transcriptional activator.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Gene
- CaNAC035t0
- Outcome
- transcriptional activatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“functions as a transcriptional activator”
t0“Molecular and Functional Characterization of CaNAC035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)”
Claim · model-written summary
Multiple abiotic stresses and hormones induced CaNAC035 expression.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Treatment
- low and high temperatures, osmotic stres, ssalt, game archives (GA), methyl-jasmonic acid (MeJA) , salicylic acid (SA), and abscisic acid (ABA)e0
- Gene
- CaNAC035e0
- Outcome
- CaNAC035 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CaNAC035 expression is induced by low and high temperatures, osmotic stres, ssalt, game archives (GA), methyl-jasmonic acid (MeJA) , salicylic acid (SA), and abscisic acid (ABA).”
t0“Molecular and Functional Characterization of CaNAC035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)”
Claim · model-written summary
Silenced pepper seedlings had more damage than wild type after cold, NaCl and mannitol.
Verbatim
e0“pepper seedlings in which CaNAC035 was down-regulated showed more damage than WT after cold, NaCl, and mannitol treatments”
Claim · model-written summary
Silenced pepper seedlings had greater electrolyte leakage and oxidative marker levels after cold.
- Taxon
- peppert0
- Treatment
- CaNAC035-silencede1
- Gene
- CaNAC035e1
- Outcome
- electrolyte leakage and higher levels of malondialdehyde (MDA), H2O2 and super oxide radicale0
- Direction
- increasede0
- Qualifier
- after cold treatmente0
Not reportedComparator
Verbatim
e0“increased electrolyte leakage and higher levels of malondialdehyde (MDA), H2O2 and super oxide radical after cold treatment”
t0“Molecular and Functional Characterization of CaNAC035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)”
e1“CaNAC035-silenced seedlings”
Claim · model-written summary
CaNAC035-silenced seedlings had lower chlorophyll content.
- Taxon
- peppert0
- Treatment
- CaNAC035-silencede0
- Gene
- CaNAC035e0
- Outcome
- chlorophyll contentse0
- Direction
- lowere0
Not reportedComparator
Verbatim
e0“CaNAC035-silenced seedlings had lower chlorophyll contents”
t0“Molecular and Functional Characterization of CaNAC035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)”
Claim · model-written summary
CaNAC035-overexpressing Arabidopsis plants had higher germination rates and fresh weights under mannitol and NaCl.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- CaNAC035-overexpressinge0
- Gene
- CaNAC035e0
- Outcome
- germination rates and fresh weightse0
- Direction
- highere0
- Qualifier
- after mannitol and NaCl treatmentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CaNAC035-overexpressing Arabidopsis plants had higher germination rates and fresh weights after mannitol and NaCl treatments”
Claim · model-written summary
Eighteen proteins were identified as possible CaNAC035 interactors.
Verbatim
e0“We also isolated 18 proteins that participate in processes such as the stress response, stress resistance, and photosynthesis, that potentially interact with CaNAC035.”
t0“Molecular and Functional Characterization of CaNAC035, an NAC Transcription Factor From Pepper (Capsicum annuum L.)”
Claim · model-written summary
The authors interpret CaNAC035 as a positive regulator of pepper abiotic stress tolerance through multiple pathways.
Verbatim
e0“CaNAC035 is a positive regulator of abiotic stress tolerance in pepper that acts through multiple pathways.”
The same questions in other papers
salicylic acid · molecular: expression · Capsicum annuum (sweet pepper)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
(-)-methyl jasmonate · molecular: expression · Capsicum annuum (sweet pepper)Positive and negative results
2 in the opposite direction
- no effect
- no effect
(+)-abscisic acid · molecular: expression · Capsicum annuum (sweet pepper)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.