Abstract
An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved by culturing flower bud, leaf segments and flower stalk segments of 80 days old field grown plants on Murashige and Skoog (MS) medium supplemented with different concentration (1.0-6.0 mg/l) of 6-benzyl adenine (BA) in combination with single concentration (1.0 mg/l) of α-naphthalene acetic acid (NAA). Lower concentration of BA (1.0 and 2.0 mg/l) with NAA induced the explants to form callus. On the other hand when the explants were cultured in higher concentration (5.0 mg/l) of BA produced shoots and 5.0 mg/l BA with 1.0 mg/l NAA was found to be the best for shoot proliferation of the three explants optimum response was obtained from flower buds. Further multiplication of shoots occurred upon transfer of shoot clumps to BA containing MS medium. Regenerated shoots were rooted in MS medium with indole-3-acetic acid (IAA) or indole-3-buteric acid (IBA) and maximum frequency (81%) of rooting with highest number (4) of roots per shoot was achieved in MS medium fortified with 0.3 mg/l IBA. The rooted shoots were acclimatized and successfully established in soil under natural environment with maximum 84% survivability.
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Claims 8
Claim · model-written summary
Lower BA (1.0 and 2.0 mg/l) with NAA induced explants to form callus.
- Taxon
- Gerbera jamesonii
Gerbera jamesoniie0 - Treatment
- Lower concentration of BA (1.0 and 2.0 mg/l) with NAAe2
- Outcome
- calluse2
- Setting
- in vitroe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved”
e2“Lower concentration of BA (1.0 and 2.0 mg/l) with NAA induced the explants to form callus”
Claim · model-written summary
Higher BA (5.0 mg/l) produced shoots from explants, whereas lower BA with NAA induced callus.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- higher concentration (5.0 mg/l) of BAe3
- Outcome
- shootse3
- Comparator
- Lower concentration of BA (1.0 and 2.0 mg/l) with NAAe2
- Setting
- in vitroe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved”
e2“Lower concentration of BA (1.0 and 2.0 mg/l) with NAA induced the explants to form callus”
e3“when the explants were cultured in higher concentration (5.0 mg/l) of BA produced shoots”
Claim · model-written summary
5.0 mg/l BA with 1.0 mg/l NAA was the best medium for shoot proliferation.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- 5.0 mg/l BA with 1.0 mg/l NAAe4
- Outcome
- best for shoot proliferatione4
- Direction
- beste4
- Setting
- in vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved”
e4“5.0 mg/l BA with 1.0 mg/l NAA was found to be the best for shoot proliferation”
Claim · model-written summary
Flower buds gave the optimum shoot proliferation response among the explants tested.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Organ / stage
- flower budse5
- Outcome
- optimum responsee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved”
e5“optimum response was obtained from flower buds”
Claim · model-written summary
Transfer of shoot clumps to BA-containing MS medium caused further multiplication of shoots.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- transfer of shoot clumps to BA containing MS mediume6
- Outcome
- multiplication of shootse6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved”
e6“Further multiplication of shoots occurred upon transfer of shoot clumps to BA containing MS medium”
Claim · model-written summary
Rooting frequency of 81% was highest in MS medium with 0.3 mg/l IBA.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- 0.3 mg/l IBAe7
- Outcome
- rootinge7
- Direction
- maximume7
- Value
- 81%e7
- Comparator
- indole-3-acetic acid (IAA)e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved”
e6“Regenerated shoots were rooted in MS medium with indole-3-acetic acid (IAA) or indole-3-buteric acid (IBA)”
e7“maximum frequency (81%) of rooting with highest number (4) of roots per shoot was achieved in MS medium fortified with 0.3 mg/l IBA”
Claim · model-written summary
Rooted shoots had the highest number of roots (4 per shoot) in MS medium with 0.3 mg/l IBA.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- 0.3 mg/l IBAe7
- Outcome
- roots per shoote7
- Direction
- higheste7
- Value
- 4e7
- Comparator
- indole-3-acetic acid (IAA)e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved”
e6“Regenerated shoots were rooted in MS medium with indole-3-acetic acid (IAA) or indole-3-buteric acid (IBA)”
e7“maximum frequency (81%) of rooting with highest number (4) of roots per shoot was achieved in MS medium fortified with 0.3 mg/l IBA”
Claim · model-written summary
Acclimatized rooted plantlets established in soil under natural environment had 84% survivability.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Outcome
- survivabilitye7
- Value
- 84%e7
- Setting
- soil under natural environmente7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved”
e7“successfully established in soil under natural environment with maximum 84% survivability”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.