Abstract
A new route of in vitro mass propagation protocol of Gerbera jamesonii (H. Bolus ex Bolus f.) derived from application of thin cell layers (TCL) and adenine sulphate (AS) was successfully developed and established. Shoot tip explants and half-strength MS medium containing 0.25 mg/l N6-benzylaminopurine (BAP), 20 g/l sucrose and 7 g/l Swallow agar were used as explant source and basic medium. Different TCL of transversal TCL (tTCL) and longitudinal TCL (lTCL) in four slicing positions of 1, 2, 3 and 4; varieties and clones i.e. G. jamesonii ‘Black Jack’, ‘Carambole’, ‘Nuance’, ‘Violente’, 01.098 and 11.46 clone; AS concentrations viz. 0, 20, 40, 60, 80 and 100 mg/l were tested in the study. Each step of in vitro culture established had unique and specific results. In the initiation stage, first slicing position of ‘Black Jack’ shoot tip tTCL was the most optimal combination treatment to produce 7.0 shoots per explant with 13.5 leaves. The first slicing position on shoot tip explants of 01.098 clone tTCL and 20 mg/l AS in half-strength MS medium containing 0.25 mg/l BAP were the most optimal combination treatment in obtaining the highest number of shoots produced per shoot up to 9.4 shoots per shoot with 34.1 leaves and 2.37 cm length of leaves in the proliferation stage, however the treatment did not give significant effect compared to control. Under periodical subcultures on the basic medium, number of shoots and leaves increased gradually from the initial culture with 3-6 shoots per shoot and 9.4-11.6 leaves till the fourth subculture with 6-11 shoots per shoot and 16.7-28.8 leaves and declined thereafter. Subculturing of shoots in accordance to produce qualified shoots for planting materials could be carried out till sixth to seventh subculture. The highest shoot multiplication rate (SMR) was established on 01.098 clone with as high as 7.3. The well shoots were easily rooted on half-strength MS medium supplemented with 0.1 mg/l BAP, 0.05 mg/l NAA and 1.5 g/l AC. Plantlets were then transferred to ex vitro condition for acclimatization on a mixture of burned-rice husk and organic manure (1:1, v/v) with 85-100% survivability. The ‘Black Jack’ and 11.46 clone were the best genotypes on the acclimatization stage with 100% survivability of plantlets. Results of the study have implication that first slicing position of shoot tip tTCL can be applied in establishing of in vitro propagation protocol for other gerberas.
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Claims 14
Claim · model-written summary
A thin cell layer and adenine sulphate protocol for mass propagation was established.
- Taxon
- Gerbera jamesonii
Gerbera jamesoniie0 - Outcome
- in vitro mass propagation protocole0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A new route of in vitro mass propagation protocol of Gerbera jamesonii (H. Bolus ex Bolus f.) derived from application of thin cell layers (TCL) and adenine sulphate (AS) was successfully developed and established.”
Claim · model-written summary
First slicing of Black Jack shoot tip tTCL produced 7.0 shoots per explant in initiation.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Material
- Black Jacke0
- Organ / stage
- shoot tipe0
- Treatment
- first slicing position of ‘Black Jack’ shoot tip tTCLe0
- Outcome
- shoots per explante0
- Direction
- most optimale0
- Value
- 7.0 shoots per explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the initiation stage, first slicing position of ‘Black Jack’ shoot tip tTCL was the most optimal combination treatment to produce 7.0 shoots per explant with 13.5 leaves.”
t0“Gerbera jamesonii”
Claim · model-written summary
That initiation treatment produced 13.5 leaves.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Material
- Black Jacke0
- Treatment
- first slicing position of ‘Black Jack’ shoot tip tTCLe0
- Outcome
- leavese0
- Direction
- most optimale0
- Value
- 13.5 leavese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the initiation stage, first slicing position of ‘Black Jack’ shoot tip tTCL was the most optimal combination treatment to produce 7.0 shoots per explant with 13.5 leaves.”
t0“Gerbera jamesonii”
Claim · model-written summary
First slice 01.098 tTCL with 20 mg/l AS gave the highest shoot count, 9.4 per shoot, without a significant difference from control.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Material
- 01.098 clonee0
- Treatment
- 20 mg/l AS in half-strength MS medium containing 0.25 mg/l BAPe0
- Outcome
- number of shoots produced per shoote0
- Direction
- higheste0
- Value
- 9.4 shoots per shoote0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The first slicing position on shoot tip explants of 01.098 clone tTCL and 20 mg/l AS in half-strength MS medium containing 0.25 mg/l BAP were the most optimal combination treatment in obtaining the highest number of shoots produced per shoot up to 9.4 shoots per shoot with 34.1 leaves and 2.37 cm length of leaves in the proliferation stage, however the treatment did not give significant effect compared to control.”
t0“Gerbera jamesonii”
Claim · model-written summary
The same proliferation combination gave 34.1 leaves without a significant difference from control.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Material
- 01.098 clonet1
- Treatment
- 20 mg/l ASt1
- Outcome
- leavese0
- Value
- 34.1 leavese0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with 34.1 leaves and 2.37 cm length of leaves in the proliferation stage, however the treatment did not give significant effect compared to control.”
t0“Gerbera jamesonii”
t1“01.098 clone tTCL and 20 mg/l AS”
Claim · model-written summary
The same proliferation combination gave 2.37 cm leaf length without a significant difference from control.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Material
- 01.098 clonet1
- Treatment
- 20 mg/l ASt1
- Outcome
- length of leavese0
- Value
- 2.37 cme0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with 34.1 leaves and 2.37 cm length of leaves in the proliferation stage, however the treatment did not give significant effect compared to control.”
t0“Gerbera jamesonii”
t1“01.098 clone tTCL and 20 mg/l AS”
Claim · model-written summary
Shoot number rose through the fourth subculture then declined.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Outcome
- number of shootse0
- Direction
- increased graduallye0
- Value
- 6-11 shoots per shoote0
- Qualifier
- fourth subculturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“number of shoots and leaves increased gradually from the initial culture with 3-6 shoots per shoot and 9.4-11.6 leaves till the fourth subculture with 6-11 shoots per shoot and 16.7-28.8 leaves and declined thereafter.”
t0“Gerbera jamesonii”
Claim · model-written summary
Leaf number rose through the fourth subculture then declined.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Outcome
- leavese0
- Direction
- increased graduallye0
- Value
- 16.7-28.8 leavese0
- Qualifier
- fourth subculturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“number of shoots and leaves increased gradually from the initial culture with 3-6 shoots per shoot and 9.4-11.6 leaves till the fourth subculture with 6-11 shoots per shoot and 16.7-28.8 leaves and declined thereafter.”
t0“Gerbera jamesonii”
Claim · model-written summary
The authors consider shoot subculturing suitable through the sixth or seventh subculture.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Outcome
- Subculturing of shoots in accordance to produce qualified shoots for planting materials could be carried out till sixth to seventh subculturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subculturing of shoots in accordance to produce qualified shoots for planting materials could be carried out till sixth to seventh subculture.”
t0“Gerbera jamesonii”
Claim · model-written summary
Clone 01.098 reached the highest shoot multiplication rate of 7.3.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Material
- 01.098 clonee0
- Outcome
- shoot multiplication ratee0
- Direction
- higheste0
- Value
- 7.3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoot multiplication rate (SMR) was established on 01.098 clone with as high as 7.3.”
t0“Gerbera jamesonii”
Claim · model-written summary
Shoots rooted readily on the stated medium.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Treatment
- half-strength MS medium supplemented with 0.1 mg/l BAP, 0.05 mg/l NAA and 1.5 g/l ACe0
- Outcome
- easily rootede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The well shoots were easily rooted on half-strength MS medium supplemented with 0.1 mg/l BAP, 0.05 mg/l NAA and 1.5 g/l AC.”
t0“Gerbera jamesonii”
Claim · model-written summary
Plantlets had 85-100% survival after transfer to the ex vitro mixture.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Treatment
- a mixture of burned-rice husk and organic manure (1:1, v/v)e0
- Outcome
- survivabilitye0
- Value
- 85-100%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlets were then transferred to ex vitro condition for acclimatization on a mixture of burned-rice husk and organic manure (1:1, v/v) with 85-100% survivability.”
t0“Gerbera jamesonii”
Claim · model-written summary
Black Jack and clone 11.46 each reached 100% acclimatization survival.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Material
- Black Jacke0
- Outcome
- survivability of plantletse0
- Direction
- beste0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ‘Black Jack’ and 11.46 clone were the best genotypes on the acclimatization stage with 100% survivability of plantlets.”
t0“Gerbera jamesonii”
Claim · model-written summary
The authors propose first slice shoot tip tTCL for propagation protocols in other gerberas.
- Outcome
- first slicing position of shoot tip tTCL can be applied in establishing of in vitro propagation protocol for other gerberase0
GerberaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“first slicing position of shoot tip tTCL can be applied in establishing of in vitro propagation protocol for other gerberas.”
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