Abstract
A regeneration procedure was developed for Gerbera (Gerbera jamesonii B.) using shoot explants from in vitro grown plants. The shoot explants stripped off roots and leaves were cultured on Murashige and Skoog s (MS) medium supplemented with different concentrations of 6-benzylamino-purine (BAP) and 6-furfurylamino-purine (kinetin) in the range 2.0- 3.0 mg l−1 along with 0.1 mg l−1 a- naphthalene acetic acid (NAA). The addition of BAP to culture medium was found more effective than kinetin. The addition of kinetin strongly suppressed the shoot proliferation and was more at higher concentration. MS medium with 2.5 mg l−1 BAP + 0.1 mg l−1 NAA produced highest number of better sized shoots. The shoots of more than one cm long were cultured on MS medium supplemented with varying concentrations of indole-3-butyric acid (IBA) and NAA for rooting at every 5-6 weeks. NAA induced early rooting compared to IBA. The callus development in IBA supplemented shoots suppressed the growth of roots. The shoots cultured on MS medium supplemented with 1.0 mg l−1 NAA produced extensive root system 4-5 weeks after inoculation. Plantlets with good root system were transferred to pots containing peat, perlite and fly ash + sand (l:l,v/v) mixture for hardening. The survival rate of plantlets was more in perlite medium compared to other hardening medium.
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Claims 7
Claim · model-written summary
BAP was more effective than kinetin in the Gerbera jamesonii shoot-regeneration medium.
- Taxon
- Gerbera jamesonii
Gerbera jamesoniie0 - Treatment
- addition of BAPe1
- Outcome
- more effectivee1
- Direction
- more effectivee1
- Comparator
- kinetine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A regeneration procedure was developed for Gerbera (Gerbera jamesonii B.) using shoot explants from in vitro grown plants”
e1“The addition of BAP to culture medium was found more effective than kinetin”
Claim · model-written summary
Kinetin strongly suppressed shoot proliferation in Gerbera jamesonii, more so at higher concentration.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- addition of kinetine2
- Outcome
- shoot proliferatione2
- Direction
- strongly suppressede2
- Qualifier
- more at higher concentratione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A regeneration procedure was developed for Gerbera (Gerbera jamesonii B.) using shoot explants from in vitro grown plants”
e2“The addition of kinetin strongly suppressed the shoot proliferation and was more at higher concentration”
Claim · model-written summary
MS medium with 2.5 mg/l BAP + 0.1 mg/l NAA produced the highest number of better-sized Gerbera shoots.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- 2.5 mg l−1 BAP + 0.1 mg l−1 NAAe3
- Outcome
- number of better sized shootse3
- Direction
- higheste3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A regeneration procedure was developed for Gerbera (Gerbera jamesonii B.) using shoot explants from in vitro grown plants”
e3“MS medium with 2.5 mg l−1 BAP + 0.1 mg l−1 NAA produced highest number of better sized shoots”
Claim · model-written summary
NAA induced earlier rooting of Gerbera shoots than IBA.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- NAAe4
- Outcome
- rootinge4
- Direction
- induced earlye4
- Comparator
- IBAe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A regeneration procedure was developed for Gerbera (Gerbera jamesonii B.) using shoot explants from in vitro grown plants”
e4“NAA induced early rooting compared to IBA”
Claim · model-written summary
Callus development in IBA-supplemented Gerbera shoots suppressed root growth.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Organ / stage
- calluse5
- Treatment
- IBA supplemented shootse5
- Outcome
- growth of rootse5
- Direction
- suppressede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A regeneration procedure was developed for Gerbera (Gerbera jamesonii B.) using shoot explants from in vitro grown plants”
e5“The callus development in IBA supplemented shoots suppressed the growth of roots”
Claim · model-written summary
Shoots on MS medium with 1.0 mg/l NAA produced an extensive root system 4-5 weeks after inoculation.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- 1.0 mg l−1 NAAe6
- Outcome
- extensive root systeme6
- Value
- 4-5 weekse6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A regeneration procedure was developed for Gerbera (Gerbera jamesonii B.) using shoot explants from in vitro grown plants”
e6“The shoots cultured on MS medium supplemented with 1.0 mg l−1 NAA produced extensive root system 4-5 weeks after inoculation”
Claim · model-written summary
Plantlet survival rate was higher in perlite hardening medium than in other hardening media.
- Taxon
- Gerbera jamesoniiGerbera jamesoniie0
- Treatment
- perlite mediume7
- Outcome
- survival rate of plantletse7
- Direction
- moree7
- Comparator
- other hardening mediume7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A regeneration procedure was developed for Gerbera (Gerbera jamesonii B.) using shoot explants from in vitro grown plants”
e7“The survival rate of plantlets was more in perlite medium compared to other hardening medium”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.