Abstract
Regeneration potentials in Gerbera jamesonii Bolus ex. Hook f. from tissues culture system was studied using leaf, petiole and root explants. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), alpha-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2,4-D), Indole-3-acetic acid (IAA), Indole-3-Butyric acid (IBA), N6-[2-Isopentenyl]adenine (2iP), Kinetin and Zeatin were used to initiate cultures. These plant growth regulators were added to Murashige and Skoog medium in different combinations and concentrations. Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg L(-1) BAP and 0.5 mg L(-1) NAA. Effectiveness of shoot regeneration medium, type of growth regulator used and duration of induction period were investigated. Leaf explants cultured on MS medium supplemented with 1.0 mg L(-1) BAP and 2.0 mg L(-1) 2, 4-D showed the best results for callus induction. Root explants were found to be non-regenerative in all experiments conducted. Petiole segment was identified as the best explant for regeneration of this species. Regenerated plants were rooted on Murashige and Skoog basal medium. Plantlets were then transferred to field with 75% survival rate.
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Claims 6
Claim · model-written summary
Petiole explants formed adventitious shoots on MS medium with BAP and NAA.
- Taxon
- Gerbera jamesonii
Gerbera jamesoniit0 - Organ / stage
- petiole explantse0
- Treatment
- Murashige and Skoog (MS) medium supplemented with 2.0 mg L(-1) BAP and 0.5 mg L(-1) NAAe0
- Outcome
- Adventitious shootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg L(-1) BAP and 0.5 mg L(-1) NAA.”
t0“Gerbera jamesonii”
Claim · model-written summary
Leaf explants on MS medium with BAP and 2,4-D gave the best callus induction.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Organ / stage
- Leaf explantse0
- Treatment
- MS medium supplemented with 1.0 mg L(-1) BAP and 2.0 mg L(-1) 2, 4-De0
- Outcome
- callus inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf explants cultured on MS medium supplemented with 1.0 mg L(-1) BAP and 2.0 mg L(-1) 2, 4-D showed the best results for callus induction.”
t0“Gerbera jamesonii”
Claim · model-written summary
Root explants did not regenerate in the experiments.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Organ / stage
- Root explantse0
- Outcome
- non-regenerativee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root explants were found to be non-regenerative in all experiments conducted.”
t0“Gerbera jamesonii”
Claim · model-written summary
Petiole segments were identified as the best explant for regeneration.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Organ / stage
- Petiole segmente0
- Outcome
- best explant for regeneratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Petiole segment was identified as the best explant for regeneration of this species.”
t0“Gerbera jamesonii”
Claim · model-written summary
Regenerated plants rooted on MS basal medium.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Treatment
- Murashige and Skoog basal mediume0
- Outcome
- rootede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plants were rooted on Murashige and Skoog basal medium.”
t0“Gerbera jamesonii”
Claim · model-written summary
Transferred plantlets had 75% field survival.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Outcome
- survival ratee0
- Value
- 75%e0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlets were then transferred to field with 75% survival rate.”
t0“Gerbera jamesonii”
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