Paper · 2014 · International Journal of Plant Animal and Environmental Sciences

An efficient micropropagation protocol for Gerbera jamesonii Bolus from flower buds.

M. R. Shylaja; P. Sashna V. Chinjusha; P. Nazeem

5
claims
10
verbatim quotes
9
citations
2
research areas
—
Not open access

Abstract

An highly efficient micropropagation protocol for commercial multiplication of gerbera was developed with unopened flower buds as explants. Media for culture establishment, shoot regeneration, propagule multiplication, elongation, rooting and hardening were standardized. For culture establishment and propagule multiplication MS medium supplemented with BAP 3mg/l. + NAA 0.1 mg/l was found good. Shoots were elongated in MS medium supplemented with NAA 0.1mg/l. For root morphogenesis, MS medium supplemented with IBA 1mg/l was effective with more number of roots and longer roots. Plantlets were successfully hardened in sand soil coir pith mixture. Response was genotype dependent in all stages of in vitro propagation.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium with BAP and NAA supported culture establishment and propagule multiplication.

Taxon
Gerbera jamesonii BolusGerbera jamesoniit0
Treatment
MS medium supplemented with BAP 3mg/l. + NAA 0.1 mg/le0
Outcome
culture establishment and propagule multiplicatione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera jamesonii
Gerbera jamesonii · TaxonRooivos · Public domain · Wikimedia Commons ↗

Verbatim

e0

“For culture establishment and propagule multiplication MS medium supplemented with BAP 3mg/l. + NAA 0.1 mg/l was found good.”

✓ located verbatim in the source · abstract, characters 272–396 · show in text ↑
t0

“Gerbera jamesonii Bolus”

✓ located verbatim in the source · title, characters 43–66 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoots elongated on MS medium supplemented with NAA.

Taxon
Gerbera jamesonii BolusGerbera jamesoniit0
Treatment
MS medium supplemented with NAA 0.1mg/le0
Outcome
Shoots were elongatede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera jamesonii
Gerbera jamesonii · TaxonRooivos · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Shoots were elongated in MS medium supplemented with NAA 0.1mg/l.”

✓ located verbatim in the source · abstract, characters 397–462 · show in text ↑
t0

“Gerbera jamesonii Bolus”

✓ located verbatim in the source · title, characters 43–66 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IBA supplemented MS medium promoted root morphogenesis with more and longer roots.

Taxon
Gerbera jamesonii BolusGerbera jamesoniit0
Treatment
MS medium supplemented with IBA 1mg/le0
Outcome
more number of roots and longer rootse0
Direction
moree0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera jamesonii
Gerbera jamesonii · TaxonRooivos · Public domain · Wikimedia Commons ↗

Verbatim

e0

“For root morphogenesis, MS medium supplemented with IBA 1mg/l was effective with more number of roots and longer roots.”

✓ located verbatim in the source · abstract, characters 463–582 · show in text ↑
t0

“Gerbera jamesonii Bolus”

✓ located verbatim in the source · title, characters 43–66 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Plantlets were successfully hardened in sand soil coir pith mixture.

Taxon
Gerbera jamesonii BolusGerbera jamesoniit0
Treatment
sand soil coir pith mixturee0
Outcome
Plantlets were successfully hardenede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera jamesonii
Gerbera jamesonii · TaxonRooivos · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Plantlets were successfully hardened in sand soil coir pith mixture.”

✓ located verbatim in the source · abstract, characters 583–651 · show in text ↑
t0

“Gerbera jamesonii Bolus”

✓ located verbatim in the source · title, characters 43–66 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Response varied by genotype across all in vitro propagation stages.

Taxon
Gerbera jamesonii BolusGerbera jamesoniit0
Material
genotypee0
Outcome
Responsee0
Qualifier
all stages of in vitro propagatione0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera jamesonii
Gerbera jamesonii · TaxonRooivos · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Response was genotype dependent in all stages of in vitro propagation.”

✓ located verbatim in the source · abstract, characters 652–722 · show in text ↑
t0

“Gerbera jamesonii Bolus”

✓ located verbatim in the source · title, characters 43–66 · show in text ↑

indole-3-butyric acid · growth · Gerbera jamesonii (Transvaal daisy)Positive and negative results

9 papers: 8 positive, 1 negative or no effect

7 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

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1-naphthaleneacetic acid · pest · Gerbera jamesonii (Transvaal daisy)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
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N-benzyladenine · pest · Gerbera jamesonii (Transvaal daisy)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

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in vitro · pest · Gerbera jamesonii (Transvaal daisy)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

in vitro · growth · Gerbera jamesonii (Transvaal daisy)Results agree

20 papers: 20 positive, 0 negative or no effect

19 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

16 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.