Abstract
Botrytis cinerea and recently B. mali have been identified as important postharvest pathogens of apples in British Columbia (BC), Canada. Three isolates of both B. cinerea and B. mali were studied alone and in combination by inoculating mature ‘Gala’ apple fruit to compare their potential for causing decay. The fruit were incubated at 20°C for 6 and 8 days when lesion areas were calculated from lesion diameters. The lesion areas in apples inoculated with B. cinerea ranged from 1020 to 1514 mm 2 compared to 130 to 293 mm 2 for B. mali after 6 days. Primers developed to specifically amplify B. mali or B. cinerea were used in a PCR test to determine which Botrytis spp. was present in a particular lesion and estimate the quantity of each species. Relative fluorescent intensity of DNA extracted from apple tissue co-inoculated with B. cinerea + B. mali and amplified with the B. cinerea specific primer averaged 102.3%. On the other hand, the fluorescence produced by the B. mali primer averaged only 11.6% from the same DNA samples. These results confirmed that when both B. cinerea and B. mali are mixed together, B. cinerea becomes the dominant pathogen. Accepted for publication 2 July 2010. Published 20 September 2010.
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Claims 4
Claim · model-written summary
B. cinerea caused larger apple lesions after six days than B. mali.
- Taxon
- apples
Malus domesticae0 - Treatment
- inoculated with B. cinereae0
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Second interactor
- B. maliBotrytis malie0pathogen
- Outcome
- lesion areas in applese0
- Direction
- compared toe0
- Value
- 1020 to 1514 mm 2e0
- Qualifier
- 130 to 293 mm 2e0
- Comparator
- B. malie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lesion areas in apples inoculated with B. cinerea ranged from 1020 to 1514 mm 2 compared to 130 to 293 mm 2 for B. mali after 6 days”
Claim · model-written summary
B. cinerea primer fluorescence averaged 102.3% in co-inoculated apple tissue.
- Taxon
- appleMalus domesticae0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Second interactor
- B. maliBotrytis malie0pathogen
- Outcome
- Relative fluorescent intensitye0
- Value
- 102.3%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Relative fluorescent intensity of DNA extracted from apple tissue co-inoculated with B. cinerea + B. mali and amplified with the B. cinerea specific primer averaged 102.3%”
Claim · model-written summary
B. mali primer fluorescence averaged 11.6% from the same co-inoculated apple samples.
- Taxon
- appleMalus domesticat0
- Interactor
- B. maliBotrytis malie0pathogen
- Outcome
- fluorescence produced by the B. mali primere0
- Value
- 11.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, the fluorescence produced by the B. mali primer averaged only 11.6% from the same DNA samples”
t0“Apple Lesions”
Claim · model-written summary
B. cinerea became dominant when mixed with B. mali.
- Taxon
- AppleMalus domesticat0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Second interactor
- B. maliBotrytis malie0pathogen
- Outcome
- B. cinerea becomes the dominant pathogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when both B. cinerea and B. mali are mixed together, B. cinerea becomes the dominant pathogen”
t0“Apple Lesions”
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