Abstract
In this study, silver nanoparticles effects on the sterilization of different sources of explants (young leaves, young flower stalks and young flower buds) of Gerbera as well as on the in vitro morphogenesis and their growth were investigated. The explants were sterilized and cut transversally (1 mm) with the flower stalk, square (0.5 × 0.5 cm) for the leaf sample, longitudinally (0.5 mm) for the flowers (removed the petals) and cultured on MS medium; then, the explants (contamination-free or no browning/necrosis) were transferred into MS medium supplemented with 0.02 mg/L TDZ plus 0.8 mg/L adenine, 10% coconut water, 30 g/L sucrose and 8 g/L agar in 15 days. The results showed that AgNPs at the appropriate concentration and duration treatment was effective in explant sterilization of flower bud (0.02% AgNPs and 20 min), flower stalks (0.02% AgNPs and 30 min) and young leaves (0.05% AgNPs and 20 min) after 15 days of culture. In addition, 3 types of morphogenesis including callus induction, somatic embryogenesis and direct shoot regeneration of explants derived from sterilization by AgNPs were improved as compared to that of HgCl2. In addition, research on the optimal medium for shoot multiplication, rooting as well as evaluation of acclimatization and the growth at the greenhouse were also studied. Results showed that MS medium supplemented with 2 mg/L NAA combined with 0.5 mg/L BA and 2 mg/L KIN is suitable for shoot multiplication; meanwhile, MS medium supplemented with 2 mg/L NAA improved rooting ability as well as quality of plantlets and to improving survival rate and acclimatization of Gerbera cultured in vitro.
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Claims 7
Claim · model-written summary
AgNPs at the appropriate concentration and duration effectively sterilized Gerbera flower bud explants (0.02% AgNPs for 20 min) after 15 days of culture.
- Taxon
- Gerbera jamesonii
Gerbera jamesoniit0 - Organ / stage
- flower bude1
- Treatment
- AgNPse1
- Outcome
- explant sterilizatione1
- Direction
- effectivee1
- Value
- 0.02% AgNPs and 20 mine1
- Qualifier
- at the appropriate concentration and duratione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera jamesonii”
e1“AgNPs at the appropriate concentration and duration treatment was effective in explant sterilization of flower bud (0.02% AgNPs and 20 min)”
Claim · model-written summary
AgNPs effectively sterilized Gerbera flower stalk explants (0.02% AgNPs for 30 min).
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Organ / stage
- flower stalkse2
- Treatment
- AgNPse2
- Outcome
- explant sterilizatione2
- Direction
- effectivee2
- Value
- 0.02% AgNPs and 30 mine2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera jamesonii”
e2“effective in explant sterilization of flower bud (0.02% AgNPs and 20 min), flower stalks (0.02% AgNPs and 30 min)”
Claim · model-written summary
AgNPs effectively sterilized Gerbera young leaf explants (0.05% AgNPs for 20 min).
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Organ / stage
- young leavese3
- Treatment
- AgNPse3
- Outcome
- explant sterilizatione3
- Direction
- effectivee3
- Value
- 0.05% AgNPs and 20 mine3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera jamesonii”
e3“effective in explant sterilization of flower bud (0.02% AgNPs and 20 min), flower stalks (0.02% AgNPs and 30 min) and young leaves (0.05% AgNPs and 20 min)”
Claim · model-written summary
Callus induction, somatic embryogenesis and direct shoot regeneration from explants sterilized with AgNPs were improved compared with HgCl2 sterilization.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Treatment
- sterilization by AgNPse4
- Outcome
- callus induction, somatic embryogenesis and direct shoot regeneratione4
- Direction
- improvede4
- Comparator
- HgCl2e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera jamesonii”
e4“3 types of morphogenesis including callus induction, somatic embryogenesis and direct shoot regeneration of explants derived from sterilization by AgNPs were improved as compared to that of HgCl2”
Claim · model-written summary
MS medium supplemented with 2 mg/L NAA, 0.5 mg/L BA and 2 mg/L KIN is suitable for shoot multiplication of Gerbera.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Treatment
- MS medium supplemented with 2 mg/L NAA combined with 0.5 mg/L BA and 2 mg/L KINe5
- Outcome
- shoot multiplicatione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera jamesonii”
e5“Results showed that MS medium supplemented with 2 mg/L NAA combined with 0.5 mg/L BA and 2 mg/L KIN is suitable for shoot multiplication”
Claim · model-written summary
MS medium supplemented with 2 mg/L NAA improved rooting ability and the quality of Gerbera plantlets.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Treatment
- MS medium supplemented with 2 mg/L NAAe6
- Outcome
- rooting ability as well as quality of plantletse6
- Direction
- improvede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera jamesonii”
e6“MS medium supplemented with 2 mg/L NAA improved rooting ability as well as quality of plantlets”
Claim · model-written summary
MS medium supplemented with 2 mg/L NAA improved survival rate and acclimatization of Gerbera cultured in vitro.
- Taxon
- Gerbera jamesoniiGerbera jamesoniit0
- Treatment
- MS medium supplemented with 2 mg/L NAAe7
- Outcome
- survival rate and acclimatizatione7
- Direction
- improvinge7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera jamesonii”
e7“MS medium supplemented with 2 mg/L NAA improved rooting ability as well as quality of plantlets and to improving survival rate and acclimatization of Gerbera cultured in vitro”
The same questions in other papers
in vitro · survival · Gerbera jamesonii (Transvaal daisy)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.