Abstract
RNAi is a major antiviral defense response in plant and animal model systems. RNA-dependent RNA polymerase 6 (RDR6) is an essential component of RNAi, which plays an important role in the resistance against viruses in the model plants. We found previously that rice RDR6 (OsRDR6) functioned in the defense against Rice stripe virus (RSV), and Rice Dwarf Phytoreovirus (RDV) infection resulted in down-regulation of expression of RDR6. Here we report our new findings on the function of OsRDR6 against RDV. Our result showed that down-regulation of OsRDR6 through the antisense (OsRDR6AS) strategy increased rice susceptibility to RDV infection while over-expression of OsRDR6 had no effect on RDV infection. The accumulation of RDV vsiRNAs was reduced in the OsRDR6AS plants. In the OsRDR6 over-expressed plants, the levels of OsRDR6 RNA transcript and protein were much higher than that in the control plants. Interestingly, the accumulation level of OsRDR6 protein became undetectable after RDV infection. This finding indicated that the translation and/or stability of OsRDR6 protein were negatively impacted upon RDV infection. This new finding provides a new light on the function of RDR6 in plant defense response and the cross-talking between factors encoded by host plant and double-stranded RNA viruses.
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Claims 6
Claim · model-written summary
Antisense down-regulation of OsRDR6 increased rice susceptibility to RDV infection.
- Taxon
- rice
Oryza sativae0 - Treatment
- down-regulation of OsRDR6 through the antisense (OsRDR6AS) strategye0
- Interactor
- RDVe0pathogen
- Gene
- OsRDR6e0
- Outcome
- susceptibility to RDV infectione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“down-regulation of OsRDR6 through the antisense (OsRDR6AS) strategy increased rice susceptibility to RDV infection”
Claim · model-written summary
OsRDR6 over-expression had no effect on RDV infection of rice.
- Taxon
- riceOryza sativae0
- Treatment
- over-expression of OsRDR6e0
- Interactor
- RDVe0pathogen
- Gene
- OsRDR6e0
- Outcome
- RDV infectione0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased rice susceptibility to RDV infection while over-expression of OsRDR6 had no effect on RDV infection.”
Claim · model-written summary
RDV vsiRNA accumulation was reduced in OsRDR6AS plants.
- Taxon
- riceOryza sativae1
- Treatment
- OsRDR6ASe0
- Interactor
- RDVe0pathogen
- Outcome
- accumulation of RDV vsiRNAse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of RDV vsiRNAs was reduced in the OsRDR6AS plants.”
e1“rice RDR6 (OsRDR6)”
Claim · model-written summary
OsRDR6 over-expressed rice plants had much higher transcript and protein levels than controls.
- Taxon
- riceOryza sativae1
- Treatment
- OsRDR6 over-expressede0
- Gene
- OsRDR6e0
- Outcome
- levels of OsRDR6 RNA transcript and proteine0
- Direction
- much highere0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the OsRDR6 over-expressed plants, the levels of OsRDR6 RNA transcript and protein were much higher than that in the control plants.”
e1“rice RDR6 (OsRDR6)”
Claim · model-written summary
OsRDR6 protein became undetectable after RDV infection.
- Taxon
- riceOryza sativae1
- Treatment
- RDV infectione0
- Interactor
- RDVe0pathogen
- Gene
- OsRDR6e0
- Outcome
- accumulation level of OsRDR6 proteine0
- Direction
- became undetectablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation level of OsRDR6 protein became undetectable after RDV infection.”
e1“rice RDR6 (OsRDR6)”
Claim · model-written summary
The authors infer that RDV infection negatively affects OsRDR6 protein translation and/or stability.
- Taxon
- riceOryza sativae1
- Treatment
- RDV infectione0
- Interactor
- RDVe0pathogen
- Gene
- OsRDR6e0
- Outcome
- translation and/or stability of OsRDR6 proteine0
- Direction
- negatively impactede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This finding indicated that the translation and/or stability of OsRDR6 protein were negatively impacted upon RDV infection.”
e1“rice RDR6 (OsRDR6)”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.