Paper · 2002 · Journal of Integrative Plant Biology

Analysis of Resistance-related Proteins in Rice Against Brown Planthopper by Two-dimensional Electrophoresis

Rongzhi Chen; Weng Qing Mei; Huang Zhen; et al.

3
claims
5
verbatim quotes
6
citations
2
research areas
—
Not open access

Abstract

A recombinant inbred population (RI) was constructed from a cross between B5, an introgression line from the wild rice Oryza officinalis Wall. ex Watt, and susceptible cultivar Minghui 63 (O. sativa L.). The brown planthopper (BPH) resistances of RI lines were evaluated. Based on bulked segregant analysis (BSA), two protein bulks were made by extracting proteins from equally mixed seedlings of extremely resistant and susceptible plants selected from the RI population, respectively. Two-dimensional electrophoresis was used to detect the changes of polypeptide pattern. Results showed that a protein P40 (pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk. In connection with the physiological changes of the resistant and susceptible lines subjected to BPH sucking, we suppose that the protein P40 is related to the interaction responses of rice plants to BPH infestation.

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Claims 3

Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Protein P40 was significantly reduced or vanished after brown planthopper infestation for 48 h in the susceptible bulk.

Material
susceptible bulke1
Treatment
BPH infestation for 48 he1
Interactor
BPHe1pest
Gene
protein P40e1
Outcome
protein P40e1
Direction
reducede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“Analysis of Resistance-related Proteins in Rice Against Brown Planthopper by Two-dimensional Electrophoresis”

✓ located verbatim in the source · title, characters 0–108 · show in text ↑
e1

“Results showed that a protein P40 (pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk”

✓ located verbatim in the source · abstract, characters 574–773 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Protein P40 remained uninfluenced after brown planthopper infestation in the resistant bulk.

Material
resistant bulke1
Treatment
BPH infestation for 48 he1
Interactor
BPHe1pest
Gene
protein P40e1
Outcome
protein P40e1
Direction
remained uninfluencede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“Analysis of Resistance-related Proteins in Rice Against Brown Planthopper by Two-dimensional Electrophoresis”

✓ located verbatim in the source · title, characters 0–108 · show in text ↑
e1

“Results showed that a protein P40 (pI 6.3, Mw 40 kD) was significantly reduced or vanished after BPH infestation for 48 h in the susceptible bulk, while it remained uninfluenced in the resistant bulk”

✓ located verbatim in the source · abstract, characters 574–773 · show in text ↑
Mechanismhypothesisoriginal resultmeasured

Claim · model-written summary

Protein P40 is suspected to be related to rice interaction responses to brown planthopper infestation.

Interactor
BPHe2pest
Gene
protein P40e2
Outcome
interaction responses of rice plants to BPH infestatione2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e2

“we suppose that the protein P40 is related to the interaction responses of rice plants to BPH infestation”

✓ located verbatim in the source · abstract, characters 885–990 · show in text ↑

infection · quality: protein · Oryza sativa (rice)Results agree

5 papers: 0 positive, 5 negative or no effect

4 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.