Abstract
To optimize conditions for an efficient embryogenic callus induction and plant regeneration from mature seeds of japonica rice cultivar Xiushui 11 and indica cultivar XC95, different factors such as plant growth regulators, casein hydrolysate, carbohydrate source and concentration, and various gelation conditions including gel type for callus induction and phyta gel concentration for shoot regeneration were studied in detail to determine their role in the system. Overall, induction of embryogenic callus in case of Xiushui 11 was the most efficient (67.2%) in the media supplemented with 2 mg/L 2,4-D, 3% sucrose and 0.8% agar while in case of XC95, 2 mg/L of 2,4-D combined with 0.5 mg/L of BAP and NAA generated the best result (61.2%). Inclusion of 0.3% agarose (pure form) or phyta gel further enhanced the callus induction efficiency (80%) in Xiusui 11. Higher concentration (300-500 mg/L) of casein hydrolysate was found to significantly inhibit the browning of calli in XC95. Role of cytokinin and phyta gel concentration was critical for shoot regeneration in both classes of rice. Increased strength of phyta gel was observed to enhance the shoot regeneration frequency. The optimal concentration of 2 mg/L and 3 mg/L for KT was necessary for higher differentiation frequency supplemented with 0.5% and 0.6% phyta gel in case of japonica and indica rice, respectively.
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Claims 8
Claim · model-written summary
Xiushui 11 reached 67.2% callus induction with 2,4-D, sucrose, and agar medium.
- Taxon
- Rice
Oryza sativat0 - Material
- Xiushui 11e0
- Treatment
- media supplemented with 2 mg/L 2,4-D, 3% sucrose and 0.8% agare0
- Outcome
- induction of embryogenic calluse0
- Direction
- most efficiente0
- Value
- 67.2%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induction of embryogenic callus in case of Xiushui 11 was the most efficient (67.2%) in the media supplemented with 2 mg/L 2,4-D, 3% sucrose and 0.8% agar”
t0“Rice”
Claim · model-written summary
XC95 reached 61.2% callus induction with 2,4-D, BAP, and NAA.
- Taxon
- RiceOryza sativat0
- Material
- XC95e0
- Treatment
- 2 mg/L of 2,4-D combined with 0.5 mg/L of BAP and NAAe0
- Outcome
- best resulte0
- Direction
- beste0
- Value
- 61.2%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in case of XC95, 2 mg/L of 2,4-D combined with 0.5 mg/L of BAP and NAA generated the best result (61.2%)”
t0“Rice”
Claim · model-written summary
Agarose or phyta gel increased Xiushui 11 callus induction efficiency to 80%.
- Taxon
- RiceOryza sativat0
- Material
- Xiusui 11e0
- Treatment
- 0.3% agarose (pure form) or phyta gele0
- Outcome
- callus induction efficiencye0
- Direction
- enhancede0
- Value
- 80%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inclusion of 0.3% agarose (pure form) or phyta gel further enhanced the callus induction efficiency (80%) in Xiusui 11”
t0“Rice”
Claim · model-written summary
High casein hydrolysate concentration significantly inhibited callus browning in XC95.
- Taxon
- RiceOryza sativat0
- Material
- XC95e0
- Treatment
- Higher concentration (300-500 mg/L) of casein hydrolysatee0
- Outcome
- browning of callie0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher concentration (300-500 mg/L) of casein hydrolysate was found to significantly inhibit the browning of calli in XC95”
t0“Rice”
Claim · model-written summary
Cytokinin and phyta gel concentrations were critical for rice shoot regeneration.
- Taxon
- RiceOryza sativat0
- Treatment
- cytokinin and phyta gel concentratione0
- Outcome
- shoot regeneratione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Role of cytokinin and phyta gel concentration was critical for shoot regeneration in both classes of rice”
t0“Rice”
Claim · model-written summary
Increased phyta gel strength enhanced shoot regeneration frequency.
- Taxon
- RiceOryza sativat0
- Treatment
- Increased strength of phyta gele0
- Outcome
- shoot regeneration frequencye0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increased strength of phyta gel was observed to enhance the shoot regeneration frequency”
t0“Rice”
Claim · model-written summary
For japonica rice, 2 mg/L KT with 0.5% phyta gel supported higher differentiation frequency.
- Taxon
- RiceOryza sativat0
- Material
- japonicae0
- Treatment
- 2 mg/Le0
- Outcome
- differentiation frequencye0
- Direction
- highere0
- Qualifier
- 0.5% and 0.6% phyta gele0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal concentration of 2 mg/L and 3 mg/L for KT was necessary for higher differentiation frequency supplemented with 0.5% and 0.6% phyta gel in case of japonica and indica rice, respectively”
t0“Rice”
Claim · model-written summary
For indica rice, 3 mg/L KT with 0.6% phyta gel supported higher differentiation frequency.
- Taxon
- RiceOryza sativat0
- Material
- indicae0
- Treatment
- 3 mg/Le0
- Outcome
- differentiation frequencye0
- Direction
- highere0
- Qualifier
- 0.5% and 0.6% phyta gele0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal concentration of 2 mg/L and 3 mg/L for KT was necessary for higher differentiation frequency supplemented with 0.5% and 0.6% phyta gel in case of japonica and indica rice, respectively”
t0“Rice”
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- no effect
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.