Paper · 2018 · Journal of Functional And Environmental Botany

Tissue Culture Studies for Micropropagation of Pelargonium graveolens L’ Herit

Nahida Tun Nisa

3
claims
6
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

An efficient protocol has been developed for direct regeneration of Pelargonium graveolens L’ Herit. Murashige and Skoog (MS)basal medium supplemented with 2.0 mg l–1 BAP and 1.0 mg l–1 IAA was optimum for attaining maximum number of shoots (12±0.94) having 60% regeneration. In vitro grown shoots of direct origin were transferred to ½ strength MS medium fortified with 1.0 mg l–1 IBA for attaining the maximum number of roots (11.0 ± 1.65) having 60% regeneration. Regenerated plantlets with well-developed shoots and roots were successfully transferred to soil. The study demonstrated an efficient in vitro procedure for micropropagation via nodal explants in P. graveolens L’ Herit which could be useful for large scale proliferation of this cash crop. Essential oil of graveolens L’ Herit is widely used in high grade perfumery, cosmetic industries, as a flavouring agent in many food categories and soft drinks. The oil has anti-bacterial and insecticidal properties and is profusely used in aroma therapy.

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Claims 3

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

MS medium with BAP and IAA produced the maximum shoot number and 60% regeneration.

Taxon
Pelargonium graveolens L’ HeritPelargonium graveolenst0
Treatment
2.0 mg l–1 BAP and 1.0 mg l–1 IAAe0
Outcome
number of shootse0
Direction
maximume0
Value
12±0.94e0
Qualifier
60% regeneratione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium graveolens
Pelargonium graveolens · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Murashige and Skoog (MS)basal medium supplemented with 2.0 mg l–1 BAP and 1.0 mg l–1 IAA was optimum for attaining maximum number of shoots (12±0.94) having 60% regeneration”

✓ located verbatim in the source · abstract, characters 101–274 · show in text ↑
t0

“Pelargonium graveolens L’ Herit”

✓ located verbatim in the source · title, characters 47–78 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Half-strength MS with IBA produced the maximum root number and 60% regeneration.

Taxon
Pelargonium graveolens L’ HeritPelargonium graveolenst0
Treatment
1.0 mg l–1 IBAe0
Outcome
number of rootse0
Direction
maximume0
Value
11.0 ± 1.65e0
Qualifier
60% regeneratione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium graveolens
Pelargonium graveolens · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“½ strength MS medium fortified with 1.0 mg l–1 IBA for attaining the maximum number of roots (11.0 ± 1.65) having 60% regeneration”

✓ located verbatim in the source · abstract, characters 335–465 · show in text ↑
t0

“Pelargonium graveolens L’ Herit”

✓ located verbatim in the source · title, characters 47–78 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Regenerated plantlets were successfully transferred to soil.

Taxon
Pelargonium graveolens L’ HeritPelargonium graveolenst0
Outcome
successfully transferred to soile0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium graveolens
Pelargonium graveolens · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Regenerated plantlets with well-developed shoots and roots were successfully transferred to soil”

✓ located verbatim in the source · abstract, characters 467–563 · show in text ↑
t0

“Pelargonium graveolens L’ Herit”

✓ located verbatim in the source · title, characters 47–78 · show in text ↑

N-benzyladenine · growth · Pelargonium graveolens (rose geranium)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

indole-3-butyric acid · growth · Pelargonium graveolens (rose geranium)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.