Abstract
In vitro somaclonal variation via callus is considered as one of the most effective tool for improvement of vegetative propagated crops. Different combinations of cytokinine (BA) and auxine (NAA) were used to induce somaclonal variation from leaf explants of the Egyptian geranium cultivar, Pelargonium graveolens L’Herit, Ait. El Qanater El khayria cv.8. Callus induction and regenerated shoots from callus were evaluated; 2.5 mg/L BA and 0.5 mg/L NAA was the optimal for callus induction and number of regenerated shoots. The obtained plantlets were acclimatized under glasshouse conditions with 80% to 90% survival and were transferred to the open field. The evaluation of growth traits and oil analysis were done for the new 13 clones compared with the mother plant in randomized block design for two successive seasons. Characterization of these new clones for morphological traits and essential oil yield and quality demonstrated the induction of variability in negative and positive directions in some characteristics in comparison with the mother plant. Clone no.7 was the superior clone in herb 2264.7 & 2357.3 g/plant and oil yield 2.436 & 3.065 ml/plant in first and second season, respectively, than the mother plant and other new clones with acceptable content of citronellol 25.63% and geraniol 26.52% as a parameter for volatile oil quality. The genetic variation of the new geranium clones were tested using SRAP technique based on PCR, the polymorphism was detected using 16 pairs of SRAP primers in combinations as forward and reverse. The genetic differences between new clones and the mother plant were ascertained with SRAP primer pair (me2 em4); a unique profile for clone no.7 was detected in SRAP analysis. The similarity percentage varied between 66.7% to 100% homologies among the new clones and their mother plant. The presence of genetic variation between new clones and the mother plant proved that the calliclones were somaclonal mutants, and the geranium cultivar, El Qanater El khayria cv.8, was improved for high yield in herb and essential oil.
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Claims 11
Claim · model-written summary
2.5 mg/L BA and 0.5 mg/L NAA was optimal for callus induction and number of regenerated shoots from leaf explants, compared with other BA and NAA combinations.
- Taxon
- Pelargonium graveolens
_Pelargonium_graveolens_flower_and_leaves.jpg?width=60)
Pelargonium graveolenst0 - Organ / stage
- leaf explantse1
- Treatment
- 2.5 mg/L BA and 0.5 mg/L NAAe2
- Outcome
- callus induction and number of regenerated shootse2
- Direction
- optimale2
- Comparator
- Different combinations of cytokinine (BA) and auxine (NAA)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Pelargonium_graveolens_flower_and_leaves.jpg?width=500)
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e1“Different combinations of cytokinine (BA) and auxine (NAA) were used to induce somaclonal variation from leaf explants”
e2“2.5 mg/L BA and 0.5 mg/L NAA was the optimal for callus induction and number of regenerated shoots”
Claim · model-written summary
Acclimatised plantlets under glasshouse conditions had 80% to 90% survival.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Outcome
- survivale3
- Value
- 80% to 90%e3
- Setting
- glasshouse conditionse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e3“The obtained plantlets were acclimatized under glasshouse conditions with 80% to 90% survival”
Claim · model-written summary
Clone no.7 was superior to the mother plant in herb yield, at 2264.7 and 2357.3 g/plant in the first and second seasons.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Material
- Clone no.7e4
- Outcome
- herbe4
- Value
- 2264.7 & 2357.3 g/plante4
- Qualifier
- in first and second seasone4
- Comparator
- the mother plante4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e4“Clone no.7 was the superior clone in herb 2264.7 & 2357.3 g/plant and oil yield 2.436 & 3.065 ml/plant in first and second season, respectively, than the mother plant”
Claim · model-written summary
Clone no.7 was superior to the mother plant in oil yield, at 2.436 and 3.065 ml/plant in the first and second seasons.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Material
- Clone no.7e4
- Outcome
- oil yielde4
- Value
- 2.436 & 3.065 ml/plante4
- Qualifier
- in first and second seasone4
- Comparator
- the mother plante4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e4“Clone no.7 was the superior clone in herb 2264.7 & 2357.3 g/plant and oil yield 2.436 & 3.065 ml/plant in first and second season, respectively, than the mother plant”
Claim · model-written summary
Clone no.7 had a citronellol content of 25.63% in its volatile oil.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Material
- Clone no.7e4
- Outcome
- content of citronellole5
- Value
- 25.63%e5
- Qualifier
- as a parameter for volatile oil qualitye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e4“Clone no.7 was the superior clone in herb 2264.7 & 2357.3 g/plant and oil yield 2.436 & 3.065 ml/plant in first and second season, respectively, than the mother plant”
e5“with acceptable content of citronellol 25.63% and geraniol 26.52% as a parameter for volatile oil quality”
Claim · model-written summary
Clone no.7 had a geraniol content of 26.52% in its volatile oil.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Material
- Clone no.7e4
- Outcome
- geraniole5
- Value
- 26.52%e5
- Qualifier
- as a parameter for volatile oil qualitye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e4“Clone no.7 was the superior clone in herb 2264.7 & 2357.3 g/plant and oil yield 2.436 & 3.065 ml/plant in first and second season, respectively, than the mother plant”
e5“with acceptable content of citronellol 25.63% and geraniol 26.52% as a parameter for volatile oil quality”
Claim · model-written summary
Clone no.7 had a unique SRAP profile.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Material
- clone no.7e6
- Outcome
- unique profilee6
- Qualifier
- SRAP analysise6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e6“a unique profile for clone no.7 was detected in SRAP analysis”
Claim · model-written summary
Similarity among the new clones and their mother plant ranged from 66.7% to 100%.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Outcome
- similarity percentagee7
- Value
- 66.7% to 100%e7
- Comparator
- their mother plante7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e7“The similarity percentage varied between 66.7% to 100% homologies among the new clones and their mother plant”
Claim · model-written summary
The new clones showed variability in negative and positive directions for some characteristics compared with the mother plant.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Outcome
- induction of variability in negative and positive directions in some characteristicse1
- Comparator
- the mother plante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e1“demonstrated the induction of variability in negative and positive directions in some characteristics in comparison with the mother plant”
Claim · model-written summary
The geranium cultivar El Qanater El khayria cv.8 was improved for high herb and essential oil yield.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Material
- El Qanater El khayria cv.8e1
- Outcome
- improved for high yield in herb and essential oile1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e1“the geranium cultivar, El Qanater El khayria cv.8, was improved for high yield in herb and essential oil”
Claim · model-written summary
The calliclones were somaclonal mutants, as shown by their genetic variation from the mother plant.
- Taxon
- Pelargonium graveolensPelargonium graveolenst0
- Outcome
- calliclones were somaclonal mutantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“PELARGONIUM GRAVEOLENS”
e1“proved that the calliclones were somaclonal mutants”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.