Abstract
The importance of lipid peroxidation and its contributing pathways (via reactive oxygen species and lipoxygenase) during post-anoxia was evaluated with respect to the biphasic behavior of membrane lipids under anoxia (A. Rawyler, D. Pavelic, C. Gianinazzi, J. Oberson, R. Brändle [1999] Plant Physiol 120: 293-300), using potato (Solanum tuberosum cv Bintje) cell cultures. When anoxic cells in the pre-lytic phase were re-oxygenated for 2 h, superoxide anion was not detectable, the hydrogen peroxide (H(2)O(2)) level remained small and similar to that of controls, and cell viability was preserved. Lipids were intact and no lipid hydroperoxides were detected. However, small amounts of lipid hydroperoxides accumulated upon feeding anoxic cells with H(2)O(2) and incubation for an additional 2 h under anoxia. When cells that entered the lytic phase of anoxia were re-oxygenated for 2 h, the H(2)O(2) and superoxide anion levels were essentially unchanged. However, cell respiration decreased, reflecting the extensive lipid hydrolysis that had already started under anoxia and continued during post-anoxia. Simultaneous with the massive release of free polyunsaturated fatty acids, small amounts of lipid hydroperoxides were formed, reaching 1% to 2% of total fatty acids. Catalase and superoxide dismutase activities were not greatly affected, whereas the amount and activity of lipoxygenase tended to increase during anoxia. Lipid peroxidation in potato cells is therefore low during post-anoxia. It is mainly due to lipoxygenase, whereas the contribution of reactive oxygen species is negligible. But above all, it is a late event that occurs only when irreversible damage is already caused by the anoxia-triggered lipid hydrolysis.
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Claims 13
Claim · model-written summary
In re-oxygenated pre-lytic anoxic potato cells, superoxide anion was not detectable.
- Taxon
- potato
Solanum tuberosume0 - Outcome
- superoxide anione1
- Qualifier
- re-oxygenated for 2 he1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e1“When anoxic cells in the pre-lytic phase were re-oxygenated for 2 h, superoxide anion was not detectable, the hydrogen peroxide (H(2)O(2)) level remained small and similar to that of controls, and cell viability was preserved”
Claim · model-written summary
In re-oxygenated pre-lytic anoxic potato cells, hydrogen peroxide remained small and similar to controls.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- re-oxygenated for 2 he1
- Outcome
- hydrogen peroxidee1
- Direction
- similare1
- Comparator
- controlse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e1“When anoxic cells in the pre-lytic phase were re-oxygenated for 2 h, superoxide anion was not detectable, the hydrogen peroxide (H(2)O(2)) level remained small and similar to that of controls, and cell viability was preserved”
Claim · model-written summary
Cell viability was preserved in re-oxygenated pre-lytic anoxic potato cells.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- cell viabilitye1
- Direction
- preservede1
- Qualifier
- re-oxygenated for 2 he1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e1“When anoxic cells in the pre-lytic phase were re-oxygenated for 2 h, superoxide anion was not detectable, the hydrogen peroxide (H(2)O(2)) level remained small and similar to that of controls, and cell viability was preserved”
Claim · model-written summary
Lipids stayed intact and no lipid hydroperoxides were detected in re-oxygenated pre-lytic potato cells.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- lipid hydroperoxidese2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e2“Lipids were intact and no lipid hydroperoxides were detected”
Claim · model-written summary
Feeding anoxic potato cells with H2O2 led to small amounts of lipid hydroperoxides accumulating under anoxia.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- feeding anoxic cells with H(2)O(2)e3
- Outcome
- lipid hydroperoxidese3
- Direction
- accumulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e3“However, small amounts of lipid hydroperoxides accumulated upon feeding anoxic cells with H(2)O(2) and incubation for an additional 2 h under anoxia”
Claim · model-written summary
In cells in the lytic phase of anoxia, re-oxygenation left H2O2 and superoxide anion levels essentially unchanged.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- re-oxygenated for 2 he4
- Outcome
- H(2)O(2) and superoxide anion levelse4
- Direction
- essentially unchangede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e4“When cells that entered the lytic phase of anoxia were re-oxygenated for 2 h, the H(2)O(2) and superoxide anion levels were essentially unchanged”
Claim · model-written summary
Cell respiration decreased in lytic-phase potato cells after re-oxygenation.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- re-oxygenated for 2 he4
- Outcome
- cell respiratione5
- Direction
- decreasede5
- Qualifier
- reflecting the extensive lipid hydrolysis that had already started under anoxiae5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e4“When cells that entered the lytic phase of anoxia were re-oxygenated for 2 h, the H(2)O(2) and superoxide anion levels were essentially unchanged”
e5“However, cell respiration decreased, reflecting the extensive lipid hydrolysis that had already started under anoxia and continued during post-anoxia”
Claim · model-written summary
Lipid hydroperoxides formed at 1% to 2% of total fatty acids in lytic-phase potato cells after re-oxygenation.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- lipid hydroperoxidese6
- Value
- 1% to 2% of total fatty acidse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e6“Simultaneous with the massive release of free polyunsaturated fatty acids, small amounts of lipid hydroperoxides were formed, reaching 1% to 2% of total fatty acids”
Claim · model-written summary
A massive release of free polyunsaturated fatty acids occurred in lytic-phase potato cells.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- massive release of free polyunsaturated fatty acidse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e6“Simultaneous with the massive release of free polyunsaturated fatty acids, small amounts of lipid hydroperoxides were formed, reaching 1% to 2% of total fatty acids”
Claim · model-written summary
Catalase and superoxide dismutase activities were not greatly affected by anoxia.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- anoxiae7
- Outcome
- Catalase and superoxide dismutase activitiese7
- Direction
- not greatly affectede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e7“Catalase and superoxide dismutase activities were not greatly affected, whereas the amount and activity of lipoxygenase tended to increase during anoxia”
Claim · model-written summary
The amount and activity of lipoxygenase tended to increase during anoxia.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- anoxiae7
- Gene
- lipoxygenasee7
- Outcome
- the amount and activity of lipoxygenasee7
- Direction
- tended to increasee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e7“Catalase and superoxide dismutase activities were not greatly affected, whereas the amount and activity of lipoxygenase tended to increase during anoxia”
Claim · model-written summary
Lipid peroxidation in potato cells during post-anoxia is mainly due to lipoxygenase, with a negligible contribution of reactive oxygen species.
- Taxon
- potatoSolanum tuberosume0
- Gene
- lipoxygenasee7
- Outcome
- Lipid peroxidatione7
- Qualifier
- the contribution of reactive oxygen species is negligiblee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e7“Lipid peroxidation in potato cells is therefore low during post-anoxia. It is mainly due to lipoxygenase, whereas the contribution of reactive oxygen species is negligible”
Claim · model-written summary
Lipid peroxidation is a late event that occurs only after irreversible damage from anoxia-triggered lipid hydrolysis.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- anoxia-triggered lipid hydrolysise7
- Outcome
- late evente7
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using potato (Solanum tuberosum cv Bintje) cell cultures”
e7“it is a late event that occurs only when irreversible damage is already caused by the anoxia-triggered lipid hydrolysis”
The same questions in other papers
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7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.