Abstract
Vitamin B6 is well recognized as an essential antioxidant and plays a role in stress responses. Co-expression of plant and pathogen-derived vitamin B6 genes is critical during disease development of R. solani. However, little is known about the functionality of vitamin B6 vitamers during plant-R. solani interactions and their involvement in disease tolerance. Here, we explored the possible involvement of vitamin B6 during disease progression of potato cultivars of different susceptibility levels to R. solani. A distinct pattern of gene expression, pyridoxine (PN) concentration, and fungal biomass was found in the susceptible cv. Russet Burbank and tolerant cv. Chieftain. Accumulation of reactive oxygen species (ROS) in R. solani mycelia or plant tissues applying non-fluorescence or fluorescence methods was related to up-regulation in the vitamin B6 pathway and is indicative of oxidative stress. Russet Burbank was susceptible to R. solani, which was linked to reduced amounts of VB6 content. Prior to infection, constitutive PN levels were significantly higher in Russet Burbank by 1.6 folds compared to Chieftain. Upon infection with R. solani, PN levels in infected tissues increased more in Chieftain (1.7 folds) compared to Russet Burbank (1.4 folds). R. solani AG3 infection of potato sprouts in both cultivars significantly activates the fungal and plant vitamin B6 and glutathione-S-transferase (GST) genes in a tissue-specific response. Significant fold increases of transcript abundance of the fungal genes ranged from a minimum of 3.60 (RsolSG3GST) to a maximum of 13.91 (RsolAG3PDX2) in the surrounding necrotic lesion tissues (zone 1). On the other hand, PCA showed that the top plant genes STGST and STPDX1.1 were linked to both tissues of necrotic lesions (zone 2) and their surrounding areas of necrotic lesions. Functional characterization of Arabidopsis pdx1.3 mutants challenged with R. solani provided evidence into the role of the vitamin B6 pathway in the maintenance of plant tolerance during disease progression. Overall, we demonstrate that the production of vitamin VB6 is under tight control and is an essential determinant of disease development during the interaction of R. solani with potato cultivars.
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Claims 12
Claim · model-written summary
Russet Burbank and Chieftain differed in expression, pyridoxine concentration and fungal biomass.
- Taxon
- potato
Solanum tuberosumt0 - Material
- cv. Russet Burbank and tolerant cv. Chieftaine0
- Interactor
- fungale0pathogen
- Outcome
- gene expression, pyridoxine (PN) concentration, and fungal biomasse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A distinct pattern of gene expression, pyridoxine (PN) concentration, and fungal biomass was found in the susceptible cv. Russet Burbank and tolerant cv. Chieftain.”
t0“Potato”
Claim · model-written summary
ROS accumulation in infected fungal mycelia or plant tissue was associated with vitamin B6 pathway upregulation.
- Taxon
- plante0
- Interactor
- R. solani
Rhizoctonia solanie0pathogen - Compound
- vitamin B6e0
- Outcome
- Accumulation of reactive oxygen species (ROS) in R. solani mycelia or plant tissuese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of reactive oxygen species (ROS) in R. solani mycelia or plant tissues applying non-fluorescence or fluorescence methods was related to up-regulation in the vitamin B6 pathway and is indicative of oxidative stress.”
Claim · model-written summary
Russet Burbank susceptibility to R. solani was linked to reduced VB6 content.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Russet Burbanke0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Compound
- VB6e0
- Outcome
- susceptible to R. solanie0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Russet Burbank was susceptible to R. solani, which was linked to reduced amounts of VB6 content.”
t0“Potato”
Claim · model-written summary
Before infection, Russet Burbank PN levels were 1.6 fold higher than Chieftain.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Russet Burbanke0
- Outcome
- constitutive PN levelse0
- Direction
- highere0
- Value
- 1.6 foldse0
- Comparator
- Chieftaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Prior to infection, constitutive PN levels were significantly higher in Russet Burbank by 1.6 folds compared to Chieftain.”
t0“Potato”
Claim · model-written summary
Infection increased PN levels 1.7 fold in Chieftain.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Chieftaine0
- Treatment
- infection with R. solanie0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Outcome
- PN levelse0
- Direction
- increasede0
- Value
- 1.7 foldse0
- Comparator
- Russet Burbanke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon infection with R. solani, PN levels in infected tissues increased more in Chieftain (1.7 folds) compared to Russet Burbank (1.4 folds).”
t0“Potato”
Claim · model-written summary
Infection increased PN levels 1.4 fold in Russet Burbank.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Russet Burbanke0
- Treatment
- infection with R. solanie0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Outcome
- PN levelse0
- Direction
- increasede0
- Value
- 1.4 foldse0
- Comparator
- Chieftaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon infection with R. solani, PN levels in infected tissues increased more in Chieftain (1.7 folds) compared to Russet Burbank (1.4 folds).”
t0“Potato”
Claim · model-written summary
R. solani infection activated fungal and plant vitamin B6 and GST genes in potato sprouts.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- sproutse0
- Treatment
- R. solani AG3 infectione0
- Interactor
- R. solani AG3Rhizoctonia solanie0pathogen
- Outcome
- fungal and plant vitamin B6 and glutathione-S-transferase (GST) genese0
- Direction
- activatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“R. solani AG3 infection of potato sprouts in both cultivars significantly activates the fungal and plant vitamin B6 and glutathione-S-transferase (GST) genes in a tissue-specific response.”
Claim · model-written summary
Fungal RsolSG3GST transcript abundance increased at least 3.60 fold around necrotic lesions.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- surrounding necrotic lesion tissues (zone 1)e0
- Interactor
- fungale0pathogen
- Gene
- RsolSG3GSTe0
- Outcome
- transcript abundance of the fungal genese0
- Direction
- increasese0
- Value
- 3.60e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant fold increases of transcript abundance of the fungal genes ranged from a minimum of 3.60 (RsolSG3GST) to a maximum of 13.91 (RsolAG3PDX2) in the surrounding necrotic lesion tissues (zone 1).”
t0“Potato”
Claim · model-written summary
Fungal RsolAG3PDX2 transcript abundance increased up to 13.91 fold around necrotic lesions.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- surrounding necrotic lesion tissues (zone 1)e0
- Interactor
- fungale0pathogen
- Gene
- RsolAG3PDX2e0
- Outcome
- transcript abundance of the fungal genese0
- Direction
- increasese0
- Value
- 13.91e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant fold increases of transcript abundance of the fungal genes ranged from a minimum of 3.60 (RsolSG3GST) to a maximum of 13.91 (RsolAG3PDX2) in the surrounding necrotic lesion tissues (zone 1).”
t0“Potato”
Claim · model-written summary
PCA linked plant STGST and STPDX1.1 to necrotic lesion and surrounding tissues.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- tissues of necrotic lesions (zone 2)e0
- Gene
- STGST and STPDX1.1e0
- Outcome
- linked to both tissues of necrotic lesions (zone 2) and their surrounding areas of necrotic lesionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCA showed that the top plant genes STGST and STPDX1.1 were linked to both tissues of necrotic lesions (zone 2) and their surrounding areas of necrotic lesions.”
t0“Potato”
Claim · model-written summary
Arabidopsis pdx1.3 mutants challenged with R. solani implicated the vitamin B6 pathway in tolerance maintenance.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- pdx1.3 mutantse0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Gene
- pdx1.3e0
- Outcome
- maintenance of plant tolerance during disease progressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional characterization of Arabidopsis pdx1.3 mutants challenged with R. solani provided evidence into the role of the vitamin B6 pathway in the maintenance of plant tolerance during disease progression.”
Claim · model-written summary
VB6 production is presented as a determinant of R. solani disease development in potato.
- Taxon
- potatoSolanum tuberosume0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Compound
- vitamin VB6e0
- Outcome
- disease developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“production of vitamin VB6 is under tight control and is an essential determinant of disease development during the interaction of R. solani with potato cultivars”
The same questions in other papers
Rhizoctonia solani · molecular: metabolites · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
infection · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
5 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.