Abstract
A minor phospholipid was isolated from potato (Solanum tuberosum L. cv Bintje) cells, chromatographically purified, and identified by electrospray ionization mass spectrometry as N-acylphosphatidylethanolamine (NAPE). The NAPE level was low in unstressed cells (13 +/- 4 nmol g fresh weight(-1)). According to acyl chain length, only 16/18/18 species (group II) and 18/18/18 species (group III) were present. NAPE increased up to 13-fold in anoxia-stressed cells, but only when free fatty acids (FFAs) started being released, after about 10 h of treatment. The level of groups II and III was increased by unspecific N-acylation of phosphatidylethanolamine, and new 16/16/18 species (group I) appeared via N-palmitoylation. NAPE also accumulated in aerated cells treated with NaN(3) plus salicylhydroxamate. N-acyl patterns of NAPE were dominated by 18:1, 18:2, and 16:0, but never reflected the FFA composition. Moreover, they did not change greatly after the treatments, in contrast with O-acyl patterns. Anoxia-induced NAPE accumulation is rooted in the metabolic homeostasis failure due to energy deprivation, but not in the absence of O(2), and is part of an oncotic death process. The acyl composition of basal and stress-induced NAPE suggests the existence of spatially distinct FFA and phosphatidylethanolamine pools. It reflects the specificity of NAPE synthase, the acyl composition, localization and availability of substrates, which are intrinsic cell properties, but has no predictive value as to the type of stress imposed. Whether NAPE has a physiological role depends on the cell being still alive and its compartmentation maintained during the stress period.
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Claims 15
Claim · model-written summary
A minor potato-cell phospholipid was identified as NAPE.
- Taxon
- potato
Solanum tuberosume0 - Material
- Bintjee0
- Outcome
- identified by electrospray ionization mass spectrometry as N-acylphosphatidylethanolamine (NAPE)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A minor phospholipid was isolated from potato (Solanum tuberosum L. cv Bintje) cells, chromatographically purified, and identified by electrospray ionization mass spectrometry as N-acylphosphatidylethanolamine (NAPE)”
Claim · model-written summary
Unstressed cells had a low NAPE level of 13 +/- 4 nmol g fresh weight(-1).
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- NAPE levele0
- Value
- 13 +/- 4 nmol g fresh weight(-1)e0
- Qualifier
- unstressed cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NAPE level was low in unstressed cells (13 +/- 4 nmol g fresh weight(-1))”
t0“Potato”
Claim · model-written summary
Only 16/18/18 and 18/18/18 NAPE species were present initially.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- only 16/18/18 species (group II) and 18/18/18 species (group III) were presente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“According to acyl chain length, only 16/18/18 species (group II) and 18/18/18 species (group III) were present”
t0“Potato”
Claim · model-written summary
Anoxia increased NAPE up to 13-fold after approximately 10 hours when free fatty acids began release.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- anoxia-stressede0
- Outcome
- NAPEe0
- Direction
- increasede0
- Value
- up to 13-folde0
- Qualifier
- after about 10 h of treatmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAPE increased up to 13-fold in anoxia-stressed cells, but only when free fatty acids (FFAs) started being released, after about 10 h of treatment”
t0“Potato”
Claim · model-written summary
Groups II and III increased by unspecific N-acylation of phosphatidylethanolamine.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- level of groups II and IIIe0
- Direction
- increasede0
- Qualifier
- unspecific N-acylation of phosphatidylethanolaminee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of groups II and III was increased by unspecific N-acylation of phosphatidylethanolamine”
t0“Potato”
Claim · model-written summary
New 16/16/18 NAPE species appeared through N-palmitoylation.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- new 16/16/18 species (group I) appearede0
- Qualifier
- N-palmitoylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“new 16/16/18 species (group I) appeared via N-palmitoylation”
t0“Potato”
Claim · model-written summary
NAPE accumulated after NaN(3) plus salicylhydroxamate treatment in aerated cells.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- NaN(3) plus salicylhydroxamatee0
- Outcome
- NAPEe0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAPE also accumulated in aerated cells treated with NaN(3) plus salicylhydroxamate”
t0“Potato”
Claim · model-written summary
NAPE N-acyl patterns were dominated by 18:1, 18:2 and 16:0.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- N-acyl patterns of NAPEe0
- Qualifier
- 18:1, 18:2, and 16:0e0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N-acyl patterns of NAPE were dominated by 18:1, 18:2, and 16:0”
t0“Potato”
Claim · model-written summary
N-acyl patterns did not reflect free fatty acid composition.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- never reflected the FFA compositione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but never reflected the FFA composition”
t0“Potato”
Claim · model-written summary
N-acyl patterns changed little after treatments.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- treatmentse0
- Outcome
- did not change greatlye0
- Direction
- did not change greatlye0
- Comparator
- O-acyl patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they did not change greatly after the treatments, in contrast with O-acyl patterns”
t0“Potato”
Claim · model-written summary
Anoxia-induced NAPE accumulation was attributed to energy deprivation and oncotic death, rather than lack of oxygen.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- Anoxia-induced NAPE accumulatione0
- Qualifier
- metabolic homeostasis failure due to energy deprivatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Anoxia-induced NAPE accumulation is rooted in the metabolic homeostasis failure due to energy deprivation, but not in the absence of O(2), and is part of an oncotic death process”
t0“Potato”
Claim · model-written summary
NAPE acyl composition suggested spatially distinct FFA and phosphatidylethanolamine pools.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- spatially distinct FFA and phosphatidylethanolamine poolse0
- Qualifier
- acyl composition of basal and stress-induced NAPEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The acyl composition of basal and stress-induced NAPE suggests the existence of spatially distinct FFA and phosphatidylethanolamine pools”
t0“Potato”
Claim · model-written summary
NAPE composition reflected intrinsic NAPE synthase and substrate properties.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- NAPE synthasee0
- Outcome
- intrinsic cell propertiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It reflects the specificity of NAPE synthase, the acyl composition, localization and availability of substrates, which are intrinsic cell properties”
t0“Potato”
Claim · model-written summary
NAPE acyl composition did not predict stress type.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- no predictive value as to the type of stress imposede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but has no predictive value as to the type of stress imposed”
t0“Potato”
Claim · model-written summary
A possible physiological role for NAPE depends on cells remaining viable and compartmentation being maintained.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- physiological rolee0
- Qualifier
- cell being still alive and its compartmentation maintainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Whether NAPE has a physiological role depends on the cell being still alive and its compartmentation maintained during the stress period”
t0“Potato”
The same questions in other papers
sodium azide · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
1 in the opposite direction
- no effect
flooding · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
2 in the opposite direction
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.