Abstract
Ralstonia solanacearum ( Rs ), the causal agent of bacterial wilt disease in an unusually wide range of host plants, including potato ( Solanum tuberosum ), is one of the most destructive phytopathogens that seriously reduces crop yields worldwide. Identification of defence mechanisms underlying bacterial wilt resistance is a prerequisite for biotechnological approaches to resistance breeding. Resistance to Rs has been reported only in a few potato landraces and cultivars. Our in vitro inoculation bioassays confirmed that the cultivars ‘Calalo Gaspar’ (CG) and ‘Cruza 148’ (CR) are resistant to Rs infection. Comparative transcriptome analyses of CG and CR roots, as well as of the roots of an Rs -susceptible cultivar, ‘Désirée’ (DES), were carried out two days after Rs infection, in parallel with their respective noninfected controls. In CR and DES, the upregulation of chitin interactions and cell wall-related genes was detected. The phenylpropanoid biosynthesis and glutathione metabolism pathways were induced only in CR, as confirmed by high levels of lignification over the whole stele in CR roots six days after Rs infection. At the same time, Rs infection greatly increased the concentrations of chlorogenic acid and quercetin derivatives in CG roots as it was detected using ultra-performance liquid chromatography − tandem mass spectrometry. Characteristic increases in the expression of MAP kinase signalling pathway genes and in the concentrations of jasmonic, salicylic, abscisic and indoleacetic acid were measured in DES roots. These results indicate different Rs defence mechanisms in the two resistant potato cultivars and a different response to Rs infection in the susceptible cultivar.
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Claims 9
Claim · model-written summary
Calalo Gaspar resisted Ralstonia infection in inoculation bioassays.
- Taxon
- potato
Solanum tuberosumt0 - Material
- Calalo Gaspare0
- Outcome
- resistant to Rs infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cultivars ‘Calalo Gaspar’ (CG) and ‘Cruza 148’ (CR) are resistant to Rs infection”
t0“potato”
Claim · model-written summary
Cruza 148 resisted Ralstonia infection in inoculation bioassays.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Cruza 148e0
- Outcome
- resistant to Rs infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cultivars ‘Calalo Gaspar’ (CG) and ‘Cruza 148’ (CR) are resistant to Rs infection”
t0“potato”
Claim · model-written summary
Chitin interaction and cell wall related genes were upregulated in Cruza 148 roots.
- Taxon
- potatoSolanum tuberosumt0
- Material
- CRe0
- Outcome
- chitin interactions and cell wall-related genese0
- Direction
- upregulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In CR and DES, the upregulation of chitin interactions and cell wall-related genes was detected”
t0“potato”
Claim · model-written summary
Chitin interaction and cell wall related genes were upregulated in Désirée roots.
- Taxon
- potatoSolanum tuberosumt0
- Material
- DESe0
- Outcome
- chitin interactions and cell wall-related genese0
- Direction
- upregulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In CR and DES, the upregulation of chitin interactions and cell wall-related genes was detected”
t0“potato”
Claim · model-written summary
Phenylpropanoid biosynthesis and glutathione metabolism pathways were induced only in Cruza 148.
- Taxon
- potatoSolanum tuberosumt0
- Material
- CRe0
- Outcome
- phenylpropanoid biosynthesis and glutathione metabolism pathwayse0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phenylpropanoid biosynthesis and glutathione metabolism pathways were induced only in CR”
t0“potato”
Claim · model-written summary
Cruza 148 roots had high lignification across the stele six days after infection.
- Taxon
- potatoSolanum tuberosumt0
- Material
- CRe0
- Organ / stage
- rootse0
- Outcome
- lignificatione0
- Qualifier
- six days after Rs infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high levels of lignification over the whole stele in CR roots six days after Rs infection”
t0“potato”
Claim · model-written summary
Infection increased chlorogenic acid and quercetin derivative concentrations in Calalo Gaspar roots.
- Taxon
- potatoSolanum tuberosumt0
- Material
- CGe0
- Treatment
- Rs infectione0
- Outcome
- concentrations of chlorogenic acid and quercetin derivativese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rs infection greatly increased the concentrations of chlorogenic acid and quercetin derivatives in CG roots”
t0“potato”
Claim · model-written summary
Désirée roots showed increased expression of MAP kinase signalling pathway genes.
- Taxon
- potatoSolanum tuberosumt0
- Material
- DESe0
- Outcome
- expression of MAP kinase signalling pathway genese0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Characteristic increases in the expression of MAP kinase signalling pathway genes and in the concentrations of jasmonic, salicylic, abscisic and indoleacetic acid were measured in DES roots”
t0“potato”
Claim · model-written summary
Désirée roots showed increased concentrations of jasmonic, salicylic and abscisic acid.
- Taxon
- potatoSolanum tuberosumt0
- Material
- DESe0
- Outcome
- concentrations of jasmonic, salicylic, abscisic and indoleacetic acide0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Characteristic increases in the expression of MAP kinase signalling pathway genes and in the concentrations of jasmonic, salicylic, abscisic and indoleacetic acid were measured in DES roots”
t0“potato”
The same questions in other papers
infection · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
5 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.