Abstract
Poplar calli transcribed two cellulase (endo-1,4-beta-glucanase) genes, PopCel1 and PopCel2, whose mRNAs were differentially located in the growing leaves of poplar during cell wall synthesis. Histochemical and RT-PCR analyses of promoter-GUS fusion gene activities in transgenic poplar demonstrated that PopCel1 promoter-derived GUS activity was localized in the petiole and leaf veins, whereas PopCel2 was confined to mesophyll cells and disappeared from the tip during the development of leaves. Autoradiography of the leaf showed that the radioactivity of [14C]sucrose incorporated into cellulose corresponded to the combination of the sucrose-induced tissue-specific patterns of PopCel1 and PopCel2. Interestingly, 2,6-dichlorobenzonitrile (DCB) not only inhibited the incorporation of the radioactivity into cellulose, but also repressed the induction of both cellulase genes. Suppression of cellulases by expression of PopCel1 antisense cDNA or co-suppression of PopCel1 mRNA by overexpression of PopCel1 sense cDNA reduced leaf growth. Therefore, we came to the conclusion that PopCel1 and PopCel2 probably function to promote leaf growth in poplar by the endohydrolysis of 1,4-beta-glucan.
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Claims 9
Claim · model-written summary
PopCel1 and PopCel2 were transcribed in poplar calli and differentially located in growing leaves.
- Organ / stage
- growing leaves of poplare0
- Gene
- PopCel1 and PopCel2e0
- Outcome
- mRNAs were differentially locatede0
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Poplar calli transcribed two cellulase (endo-1,4-beta-glucanase) genes, PopCel1 and PopCel2, whose mRNAs were differentially located in the growing leaves of poplar during cell wall synthesis.”
Claim · model-written summary
PopCel1 promoter activity localized to the petiole and leaf veins.
- Organ / stage
- petiole and leaf veinse0
- Gene
- PopCel1e0
- Outcome
- GUS activity was localized in the petiole and leaf veinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histochemical and RT-PCR analyses of promoter-GUS fusion gene activities in transgenic poplar demonstrated that PopCel1 promoter-derived GUS activity was localized in the petiole and leaf veins, whereas PopCel2 was confined to mesophyll cells and disappeared from the tip during the development of leaves.”
Claim · model-written summary
PopCel2 activity was confined to mesophyll cells and disappeared from the leaf tip during development.
- Organ / stage
- mesophyll cellse0
- Gene
- PopCel2e0
- Outcome
- confined to mesophyll cells and disappeared from the tipe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histochemical and RT-PCR analyses of promoter-GUS fusion gene activities in transgenic poplar demonstrated that PopCel1 promoter-derived GUS activity was localized in the petiole and leaf veins, whereas PopCel2 was confined to mesophyll cells and disappeared from the tip during the development of leaves.”
Claim · model-written summary
Cellulose incorporation of radiolabeled sucrose corresponded to the combined PopCel1 and PopCel2 tissue patterns.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Autoradiography of the leaf showed that the radioactivity of [14C]sucrose incorporated into cellulose corresponded to the combination of the sucrose-induced tissue-specific patterns of PopCel1 and PopCel2.”
t0“poplar”
Claim · model-written summary
DCB inhibited radiolabel incorporation into cellulose.
- Treatment
- 2,6-dichlorobenzonitrile (DCB)e0
- Outcome
- incorporation of the radioactivity into cellulosee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interestingly, 2,6-dichlorobenzonitrile (DCB) not only inhibited the incorporation of the radioactivity into cellulose, but also repressed the induction of both cellulase genes.”
t0“poplar”
Claim · model-written summary
DCB repressed induction of both cellulase genes.
- Treatment
- 2,6-dichlorobenzonitrile (DCB)e0
- Outcome
- induction of both cellulase genese0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interestingly, 2,6-dichlorobenzonitrile (DCB) not only inhibited the incorporation of the radioactivity into cellulose, but also repressed the induction of both cellulase genes.”
t0“poplar”
Claim · model-written summary
expression of PopCel1 antisense cDNA reduced poplar leaf growth.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of cellulases by expression of PopCel1 antisense cDNA or co-suppression of PopCel1 mRNA by overexpression of PopCel1 sense cDNA reduced leaf growth.”
t0“poplar”
Claim · model-written summary
overexpression of PopCel1 sense cDNA reduced poplar leaf growth.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of cellulases by expression of PopCel1 antisense cDNA or co-suppression of PopCel1 mRNA by overexpression of PopCel1 sense cDNA reduced leaf growth.”
t0“poplar”
Claim · model-written summary
The authors conclude that PopCel1 and PopCel2 probably promote poplar leaf growth through endohydrolysis of 1,4-beta-glucan.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Therefore, we came to the conclusion that PopCel1 and PopCel2 probably function to promote leaf growth in poplar by the endohydrolysis of 1,4-beta-glucan.”
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.