Abstract
The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop.
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Claims 9
Claim · model-written summary
Pag-miR408 expression significantly enhanced saccharification in laboratory and field plants without acid pretreatment.
- Treatment
- expression of Pag-miR408e0
- Gene
- Pag-miR408e0
- Outcome
- saccharificatione0
- Direction
- enhancese0
- Qualifier
- no requirement for acid-pretreatmente0
PopulusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment”
Claim · model-written summary
Pag-miR408 expression promoted plant growth.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth”
Claim · model-written summary
Pag-miR408 overexpression increased cell-wall accessibility to cellulase and scaffoldin binding modules.
- Treatment
- overexpressione0
- Outcome
- accessibility of cell walls to cellulase and scaffoldin cellulose-binding modulese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“poplar”
e0“The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules”
Claim · model-written summary
Pag-miR408 loss of function decreased cell-wall accessibility.
- Treatment
- Pag-miR408 loss-of-functione0
- Gene
- Pag-miR408e0
- Outcome
- cell wall accessibilitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility”
Claim · model-written summary
Pag-miR408 overexpression targeted three Pag-LACCASES.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“poplar”
e0“Overexpression of Pag-miR408 targets three Pag-LACCASES”
Claim · model-written summary
Pag-miR408 overexpression delayed lignification.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“poplar”
e0“Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification”
Claim · model-written summary
Pag-miR408 overexpression modestly reduced lignin content, S/G ratio, and lignin polymerization degree.
- Treatment
- Overexpression of Pag-miR408e0
- Gene
- Pag-miR408e0
- Outcome
- lignin content, S/G ratio and degree of lignin polymerizatione0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“poplar”
e0“Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization”
Claim · model-written summary
LACCASE loss-of-function mutants had significantly increased growth.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“poplar”
e0“LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem”
Claim · model-written summary
LACCASE loss-of-function mutants had significantly increased xylem cell-wall accessibility.
- Organ / stage
- xyleme0
- Treatment
- LACCASE loss of functione0
- Gene
- LACCASEe0
- Outcome
- cell wall accessibilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“poplar”
e0“LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem”
The same questions in other papers
transgenic · growth · Populus (poplar)Positive and negative results
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- negative
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See all 5 papers →loss of function · cytology · Populus (poplar)Results agree
1 paper in the same direction as this paper
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.