Abstract
• Here, we studied the poplar C(19) gibberellin 2-oxidase (GA2ox) gene subfamily. We show that a set of paralogous gene pairs differentially regulate shoot and root development. • PtGA2ox4 and its paralogous gene PtGA2ox5 are primarily expressed in aerial organs, and overexpression of PtGA2ox5 produced a strong dwarfing phenotype characteristic of GA deficiency. Suppression of PtGA2ox4 and PtGA2ox5 led to increased biomass growth, but had no effect on root development. By contrast, the PtGA2ox2 and PtGA2ox7 paralogous pair was predominantly expressed in roots, and when these two genes were RNAi-suppressed it led to a decrease of root biomass. • The morphological changes in the transgenic plants were underpinned by tissue-specific increases in bioactive GAs that corresponded to the predominant native expression of the targeted paralogous gene pair. Although RNAi suppression of both paralogous pairs led to changes in wood development, they were much greater in the transgenics with suppressed PtGA2ox4 and PtGA2ox5. The degree of gene suppression in independent events was strongly associated with phenotypes, demonstrating dose-dependent control of growth by GA2ox RNA concentrations. • The expression and transgenic modifications reported here show that shoot- and leaf-expressed PtGA2ox4 and PtGA2ox5 specifically restrain aerial shoot growth, while root-expressed PtGA2ox2 and PtGA2ox7 promote root development.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
PtGA2ox4 and PtGA2ox5 were mainly expressed in aerial organs.
- Organ / stage
- aerial organse0
- Gene
- PtGA2ox4 and its paralogous gene PtGA2ox5e0
- Outcome
- primarily expressede0
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtGA2ox4 and its paralogous gene PtGA2ox5 are primarily expressed in aerial organs”
t0“Populus”
Claim · model-written summary
PtGA2ox5 overexpression caused pronounced dwarfing.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PtGA2ox5 produced a strong dwarfing phenotype characteristic of GA deficiency.”
t0“Populus”
Claim · model-written summary
Suppressing PtGA2ox4 and PtGA2ox5 increased biomass growth.
- Treatment
- Suppression of PtGA2ox4 and PtGA2ox5e0
- Gene
- PtGA2ox4 and PtGA2ox5e0
- Outcome
- biomass growthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of PtGA2ox4 and PtGA2ox5 led to increased biomass growth”
t0“Populus”
Claim · model-written summary
Suppressing PtGA2ox4 and PtGA2ox5 had no effect on root development.
- Treatment
- Suppression of PtGA2ox4 and PtGA2ox5e0
- Gene
- PtGA2ox4 and PtGA2ox5e0
- Outcome
- root developmente0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of PtGA2ox4 and PtGA2ox5 led to increased biomass growth, but had no effect on root development.”
t0“Populus”
Claim · model-written summary
PtGA2ox2 and PtGA2ox7 were predominantly expressed in roots.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PtGA2ox2 and PtGA2ox7 paralogous pair was predominantly expressed in roots”
t0“Populus”
Claim · model-written summary
RNAi suppression of PtGA2ox2 and PtGA2ox7 decreased root biomass.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when these two genes were RNAi-suppressed it led to a decrease of root biomass.”
t0“Populus”
Claim · model-written summary
Transgenic changes corresponded with tissue specific increases in bioactive GAs.
- Gene
- targeted paralogous gene paire0
- Outcome
- tissue-specific increases in bioactive GAse0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The morphological changes in the transgenic plants were underpinned by tissue-specific increases in bioactive GAs that corresponded to the predominant native expression of the targeted paralogous gene pair.”
t0“Populus”
Claim · model-written summary
Suppressing either gene pair altered wood development, more strongly for PtGA2ox4 and PtGA2ox5.
- Treatment
- RNAi suppression of both paralogous pairse0
- Gene
- PtGA2ox4 and PtGA2ox5e0
- Outcome
- wood developmente0
- Direction
- changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although RNAi suppression of both paralogous pairs led to changes in wood development, they were much greater in the transgenics with suppressed PtGA2ox4 and PtGA2ox5.”
t0“Populus”
Claim · model-written summary
Degree of gene suppression was strongly associated with phenotypes.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The degree of gene suppression in independent events was strongly associated with phenotypes”
t0“Populus”
The same questions in other papers
loss of function · growth · Populus (poplar)Positive and negative results
4 papers in the same direction as this paper · 10 in the opposite direction
- positive
- negative
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.