Abstract
In plants, reactive oxygen species and, more particularly, hydrogen peroxide (H(2)O(2)) play a dual role as toxic by-products of normal cell metabolism and as regulatory molecules in stress perception and signal transduction. Peroxisomal catalases are an important sink for photorespiratory H(2)O(2). Using ATH1 Affymetrix microarrays, expression profiles were compared between control and catalase-deficient Arabidopsis (Arabidopsis thaliana) plants. Reduced catalase levels already provoked differences in nuclear gene expression under ambient growth conditions, and these effects were amplified by high light exposure in a sun simulator for 3 and 8 h. This genome-wide expression analysis allowed us to reveal the expression characteristics of complete pathways and functional categories during H(2)O(2) stress. In total, 349 transcripts were significantly up-regulated by high light in catalase-deficient plants and 88 were down-regulated. From this data set, H(2)O(2) was inferred to play a key role in the transcriptional up-regulation of small heat shock proteins during high light stress. In addition, several transcription factors and candidate regulatory genes involved in H(2)O(2) transcriptional gene networks were identified. Comparisons with other publicly available transcriptome data sets of abiotically stressed Arabidopsis revealed an important intersection with H(2)O(2)-deregulated genes, positioning elevated H(2)O(2) levels as an important signal within abiotic stress-induced gene expression. Finally, analysis of transcriptional changes in a combination of a genetic (catalase deficiency) and an environmental (high light) perturbation identified a transcriptional cluster that was strongly and rapidly induced by high light in control plants, but impaired in catalase-deficient plants. This cluster comprises the complete known anthocyanin regulatory and biosynthetic pathway, together with genes encoding unknown proteins.
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Claims 9
Claim · model-written summary
Reduced catalase levels changed nuclear gene expression under ambient conditions.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- Reduced catalase levelse0
- Outcome
- nuclear gene expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced catalase levels already provoked differences in nuclear gene expression under ambient growth conditions”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
High light amplified gene expression effects of reduced catalase levels.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- high light exposuree0
- Outcome
- effectse0
- Direction
- amplifiede0
- Qualifier
- 3 and 8 he0
- Setting
- sun simulatore0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these effects were amplified by high light exposure in a sun simulator for 3 and 8 h”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
High light significantly up-regulated 349 transcripts in catalase-deficient Arabidopsis.
- Taxon
- ArabidopsisArabidopsise1
- Material
- catalase-deficient plantse0
- Treatment
- high lighte0
- Outcome
- transcriptse0
- Direction
- up-regulatede0
- Value
- 349 transcriptse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“349 transcripts were significantly up-regulated by high light in catalase-deficient plants”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
High light down-regulated 88 transcripts in catalase-deficient Arabidopsis.
- Taxon
- ArabidopsisArabidopsise2
- Material
- catalase-deficient plantse1
- Treatment
- high lighte1
- Outcome
- transcriptse1
- Direction
- down-regulatede0
- Value
- 88e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“88 were down-regulated”
e1“349 transcripts were significantly up-regulated by high light in catalase-deficient plants and 88 were down-regulated”
e2“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Hydrogen peroxide was inferred to promote small heat shock protein transcription during high light stress.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- high light stresse0
- Gene
- small heat shock proteinse0
- Compound
- H(2)O(2)e0
- Outcome
- transcriptional up-regulation of small heat shock proteinse0
- Direction
- up-regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H(2)O(2) was inferred to play a key role in the transcriptional up-regulation of small heat shock proteins during high light stress”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
The analysis identified transcription factors and candidate regulatory genes in hydrogen peroxide transcriptional networks.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- transcription factors and candidate regulatory genese0
- Outcome
- identifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several transcription factors and candidate regulatory genes involved in H(2)O(2) transcriptional gene networks were identified”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Hydrogen peroxide deregulated genes overlapped with other abiotic stress transcriptome data sets.
- Taxon
- ArabidopsisArabidopsise0
- Compound
- H(2)O(2)e0
- Outcome
- important intersectione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparisons with other publicly available transcriptome data sets of abiotically stressed Arabidopsis revealed an important intersection with H(2)O(2)-deregulated genes”
Claim · model-written summary
A high light induced transcriptional cluster in controls was impaired in catalase-deficient plants.
- Taxon
- ArabidopsisArabidopsise1
- Material
- catalase-deficient plantse0
- Treatment
- high lighte0
- Outcome
- transcriptional clustere0
- Direction
- impairede0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a transcriptional cluster that was strongly and rapidly induced by high light in control plants, but impaired in catalase-deficient plants”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
The affected cluster contained the known anthocyanin regulatory and biosynthetic pathway and genes encoding unknown proteins.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- complete known anthocyanin regulatory and biosynthetic pathway, together with genes encoding unknown proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This cluster comprises the complete known anthocyanin regulatory and biosynthetic pathway, together with genes encoding unknown proteins”
e1“Arabidopsis (Arabidopsis thaliana)”
The same questions in other papers
light · molecular: expression · ArabidopsisPositive and negative results
24 papers in the same direction as this paper · 55 in the opposite direction
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- positive
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