Abstract
A study was conducted with hypocotyl, cotyledon and shoot tip of chilli as explants for regeneration on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Regeneration potential was determined by two ways. One is regeneration of shoot via callus formation from hypocotyls and cotyledon explants; another was direct shoot regeneration from shoot tip explant. The highest callus was induced from hypocotyl in a combination of BAP (5.0 mgL-1) with NAA (0.1 mgL-1) and cotyledon in a combination of BAP (5.0 mgL-1) with IAA ((1.0 mgL-1). The callus induction as well as shoot initiation was higher in hypocotyls than cotyledon. Shoot tips regenerated into plantlets directly with sporadic small callus at the base. Shoot elongation was accelerated by using additional supplementation of GA3 and AgNO3. Regenerated shoots rooted best on the MS medium supplemented with 0.1 mg L-1 NAA + 0.05 mg L-1 IBA.
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Claims 6
Claim · model-written summary
Hypocotyl callus induction was highest with BAP at 5.0 mgL-1 plus NAA at 0.1 mgL-1.
- Taxon
- chillie1
- Organ / stage
- hypocotyle0
- Treatment
- BAP (5.0 mgL-1) with NAA (0.1 mgL-1)e0
- Outcome
- calluse0
- Direction
- higheste0
Not reportedComparator
Verbatim
e0“The highest callus was induced from hypocotyl in a combination of BAP (5.0 mgL-1) with NAA (0.1 mgL-1)”
e1“chilli as explants”
Claim · model-written summary
Cotyledon callus induction was highest with BAP at 5.0 mgL-1 plus IAA at 1.0 mgL-1.
- Taxon
- chillie2
- Organ / stage
- cotyledone0
- Treatment
- BAP (5.0 mgL-1) with IAA ((1.0 mgL-1)e0
- Outcome
- calluse1
- Direction
- higheste1
Not reportedComparator
Verbatim
e0“cotyledon in a combination of BAP (5.0 mgL-1) with IAA ((1.0 mgL-1)”
e1“The highest callus was induced”
e2“chilli as explants”
Claim · model-written summary
Hypocotyls had higher callus induction and shoot initiation than cotyledons.
Verbatim
e0“The callus induction as well as shoot initiation was higher in hypocotyls than cotyledon”
e1“chilli as explants”
Claim · model-written summary
Shoot tips regenerated directly into plantlets with small basal callus.
Verbatim
e0“Shoot tips regenerated into plantlets directly with sporadic small callus at the base”
e1“chilli as explants”
Claim · model-written summary
Additional GA3 and AgNO3 accelerated shoot elongation.
- Taxon
- chillie1
- Treatment
- additional supplementation of GA3 and AgNO3e0
- Outcome
- Shoot elongatione0
- Direction
- acceleratede0
Not reportedComparator
Verbatim
e0“Shoot elongation was accelerated by using additional supplementation of GA3 and AgNO3”
e1“chilli as explants”
Claim · model-written summary
Rooting was best on MS medium with NAA at 0.1 mg L-1 plus IBA at 0.05 mg L-1.
- Taxon
- chillie1
- Treatment
- MS medium supplemented with 0.1 mg L-1 NAA + 0.05 mg L-1 IBAe0
- Outcome
- Regenerated shoots rootede0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“Regenerated shoots rooted best on the MS medium supplemented with 0.1 mg L-1 NAA + 0.05 mg L-1 IBA”
e1“chilli as explants”
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