Abstract
An efficient and reproducible in vitro regeneration protocol for a popular chili variety Bogurar jhal morich was established. For regeneration of shoots two different explants such as cotyledonary leaf and cotyledonary node were employed using various concentrations and combinations of different growth regulators. MS medium supplemented with 26.0 M BAP was found best for shoot induction from cotyledonary node explants whereas in case of cotyledonary leaf explants, 26.0 M BAP and 2.32 M Kn hormonal combination produced best results. Cotyledonary node explants also responded best towards multiple shoot induction on MS medium supplemented with 2.72 M TDZ. MS medium supplemented with 7.2 M GA 3 and 24.0 M AgNO 3 were found to be the best media combinations for shoot elongation. Highest percentage of root induction was achieved on MS medium supplemented with 3.0 M IAA from excised shoots of chili. Following the development of roots in vitro regenerated plantlets were successfully transplanted into soil.
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Claims 7
Claim · model-written summary
A reproducible in vitro regeneration protocol was established for Bogurar jhal morich.
Not reportedComparatorValue
Verbatim
e0“An efficient and reproducible in vitro regeneration protocol for a popular chili variety Bogurar jhal morich was established”
Claim · model-written summary
MS medium with 26.0 M BAP was best for shoot induction from cotyledonary nodes.
- Taxon
- chilit0
- Organ / stage
- cotyledonary node explantse0
- Treatment
- MS medium supplemented with 26.0 M BAPe0
- Outcome
- shoot inductione0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“MS medium supplemented with 26.0 M BAP was found best for shoot induction from cotyledonary node explants”
t0“Chili Variety”
Claim · model-written summary
26.0 M BAP with 2.32 M Kn was best for shoot induction from cotyledonary leaves.
- Taxon
- chilit0
- Organ / stage
- cotyledonary leaf explantse0
- Treatment
- 26.0 M BAP and 2.32 M Kn hormonal combinatione0
- Outcome
- best resultse0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“cotyledonary leaf explants, 26.0 M BAP and 2.32 M Kn hormonal combination produced best results”
t0“Chili Variety”
Claim · model-written summary
MS medium with 2.72 M TDZ was best for multiple shoot induction from cotyledonary nodes.
- Taxon
- chilit0
- Treatment
- MS medium supplemented with 2.72 M TDZe0
- Outcome
- multiple shoot inductione0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“Cotyledonary node explants also responded best towards multiple shoot induction on MS medium supplemented with 2.72 M TDZ”
t0“Chili Variety”
Claim · model-written summary
MS medium with 7.2 M GA 3 and 24.0 M AgNO 3 was best for shoot elongation.
- Taxon
- chilit0
- Treatment
- MS medium supplemented with 7.2 M GA 3 and 24.0 M AgNO 3e0
- Outcome
- shoot elongatione0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“MS medium supplemented with 7.2 M GA 3 and 24.0 M AgNO 3 were found to be the best media combinations for shoot elongation”
t0“Chili Variety”
Claim · model-written summary
MS medium with 3.0 M IAA gave the highest root induction percentage from excised chili shoots.
- Taxon
- chilie0
- Treatment
- MS medium supplemented with 3.0 M IAAe0
- Outcome
- percentage of root inductione0
- Direction
- Higheste0
Not reportedComparator
Verbatim
e0“Highest percentage of root induction was achieved on MS medium supplemented with 3.0 M IAA from excised shoots of chili”
Claim · model-written summary
Regenerated plantlets were transplanted successfully into soil after rooting.
Verbatim
e0“Following the development of roots in vitro regenerated plantlets were successfully transplanted into soil”
t0“Chili Variety”
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