Abstract
SummaryRegeneration of well-developed shootlets from cotyledonary explants of chilli `Pusa Jwala' and `G4' was obtained in MS medium supplemented with (9–18.µM) zeatin in combination with GA3 (2.89.µM). The elongated shoots could be rooted successfully on IBA containing MS medium. Cotyledonary explants of `Pusa Jwala' were transformed with Agrobacterium tumefaciens st C58 containing binary vector pGV1040 harbouring the two reporter genes npt II and GUS. The transformed shoots were selected on regeneration medium containing kanamycin (100 mg l–1) and cefotaxime (500 mg l–1) and subsequently rooted in the presence of kanamycin (75 mg l–1). The presence of the transgene was confirmed through histochemical staining of GUS, polymerase chain reaction (PCR) and Southern hybridization analysis of npt II gene.
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Claims 5
Claim · model-written summary
Cotyledonary explants of Pusa Jwala and G4 regenerated well-developed shootlets on zeatin plus GA3.
- Taxon
- chillie0
- Material
- Pusa Jwalae0
- Organ / stage
- cotyledonary explantse0
- Treatment
- MS medium supplemented with (9–18.µM) zeatin in combination with GA3 (2.89.µM)e0
- Outcome
- Regeneration of well-developed shootletse0
Not reportedComparatorDirection
Verbatim
e0“Regeneration of well-developed shootlets from cotyledonary explants of chilli `Pusa Jwala' and `G4' was obtained in MS medium supplemented with (9–18.µM) zeatin in combination with GA3 (2.89.µM)”
Claim · model-written summary
Elongated chilli shoots rooted on IBA-containing MS medium.
- Taxon
- chilli peppert0
- Organ / stage
- elongated shootse0
- Treatment
- IBA containing MS mediume0
- Outcome
- rooted successfullye0
Not reportedComparatorDirection
Verbatim
e0“The elongated shoots could be rooted successfully on IBA containing MS medium”
t0“chilli pepper (Capsicum annuum L.)”
Claim · model-written summary
Pusa Jwala cotyledonary explants were transformed using Agrobacterium tumefaciens C58 carrying npt II and GUS.
- Taxon
- chilli peppert0
- Material
- Pusa Jwalae0
- Organ / stage
- Cotyledonary explantse0
- Interactor
- Agrobacterium tumefaciens st C58
Agrobacterium tumefaciense0other - Gene
- npt II and GUSe0
- Outcome
- were transformede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotyledonary explants of `Pusa Jwala' were transformed with Agrobacterium tumefaciens st C58 containing binary vector pGV1040 harbouring the two reporter genes npt II and GUS”
t0“chilli pepper (Capsicum annuum L.)”
Claim · model-written summary
Transformed chilli shoots were selected on kanamycin and cefotaxime and subsequently rooted on kanamycin.
Verbatim
e0“The transformed shoots were selected on regeneration medium containing kanamycin (100 mg l–1) and cefotaxime (500 mg l–1) and subsequently rooted in the presence of kanamycin (75 mg l–1)”
t0“chilli pepper (Capsicum annuum L.)”
Claim · model-written summary
GUS staining, PCR, and Southern analysis of npt II confirmed transgene presence.
Not reportedComparator
Verbatim
e0“The presence of the transgene was confirmed through histochemical staining of GUS, polymerase chain reaction (PCR) and Southern hybridization analysis of npt II gene”
t0“chilli pepper (Capsicum annuum L.)”
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.