Abstract
The in vitro culture of embryos is an important technique to enable the rescue of embryos from incompatible crosses. Studies analyzing the factors that affect the in vitro culture of zygotic embryos are scarce. This study evaluated the effect of the genotype (Capsicum baccatum and C. frutescens), the composition of the culture medium (MS and ½ MS medium with different concentrations of sucrose, IAA and GA3) and the stage of development (globular, cordiform, torpedo and cotyledonary) in the in vitro culture of immature embryos. Embryo germination was influenced primarily by the stage of development of the embryo and the composition of the culture medium. Regardless of the species, the most suitable culture medium for the germination of globular and cordiform embryos was ½ MS with 0.05 mg L-1 of GA3 and IAA and 40 g L-1 of sucrose. For torpedo and cotyledonary embryos, ½ MS culture medium with 20 g L-1 of sucrose without phytoregulators is recommended for germination. These results were better than those described in the literature for all development stages in the two species. The results in the present study will be useful for geneticists and genetic enhancers interested in applying germination techniques conducted in vitro for immature Capsicum embryos.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Embryo developmental stage primarily influenced in vitro germination.
CapsicumNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryo germination was influenced primarily by the stage of development of the embryo”
t0“Capsicum spp.”
Claim · model-written summary
Culture medium composition primarily influenced in vitro embryo germination.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryo germination was influenced primarily by the stage of development of the embryo and the composition of the culture medium”
t0“Capsicum spp.”
Claim · model-written summary
For globular and cordiform embryos, ½ MS with 0.05 mg L-1 GA3 and IAA and 40 g L-1 sucrose was the most suitable germination medium across both species.
- Organ / stage
- globular and cordiform embryose0
- Treatment
- ½ MS with 0.05 mg L-1 of GA3 and IAA and 40 g L-1 of sucrosee0
- Outcome
- germination of globular and cordiform embryose0
- Direction
- most suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regardless of the species, the most suitable culture medium for the germination of globular and cordiform embryos was ½ MS with 0.05 mg L-1 of GA3 and IAA and 40 g L-1 of sucrose”
t0“Capsicum spp.”
Claim · model-written summary
For torpedo and cotyledonary embryos, the authors recommend ½ MS with 20 g L-1 sucrose and no phytoregulators for germination.
- Organ / stage
- torpedo and cotyledonary embryose0
- Outcome
- ½ MS culture medium with 20 g L-1 of sucrose without phytoregulators is recommended for germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For torpedo and cotyledonary embryos, ½ MS culture medium with 20 g L-1 of sucrose without phytoregulators is recommended for germination”
t0“Capsicum spp.”
Claim · model-written summary
Germination results for all developmental stages in both species exceeded previously published results.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results were better than those described in the literature for all development stages in the two species”
t0“Capsicum spp.”
The same questions in other papers
indole-3-acetic acid · growth · Capsicum (peppers)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →Gibberellic acid · growth · Capsicum (peppers)Positive and negative results
14 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.