Abstract
Pre-requisite for any plant biotechnological approach is development of an efficient plant regeneration system in that crop. In this regard the in vitro regeneration was achieved by using cotyledon explants from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra. Seeds of both hybrids were exposed to different treatments for proper germination, were decontaminated and sown in vitro on half-strength MS medium. The seed soaked in distilled water along with 2 mg/l GA3 for two days prior to sowing had more pronounced effect on both capsicum hybrids recording maximum germination (90.45 and 84.59 %, respectively) in minimum number of days (9.67 days and 10.33 days, respectively). Regeneration potential of cotyledonary explants touching the medium both abaxial and adaxial sides was examined. Tissue culture responses varied with the genotypes, side of explants touching the medium and combinations of growth regulators used. Regeneration per cent (96.30%), number of shoots per explants (4.56) and per cent shoot elongation (82.10%) was maximum in hybrid Indra from cotyledon explant having abaxial side in contact with growing medium supplemented with 7.5 mg/l zeatin along with 2.0 mg/l GA3. When regenerated shoots cultured on MS media supplemented with 0.5 mg/l IBA produced 100% rooting in both the hybrids. The survival percentage of regenerated plantlets during hardening in pots containing sterile mixture of 1:1 coco-peat and vermiculite was higher in Indra (92.31%) as compared to Bharat (85.71%).
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Claims 10
Claim · model-written summary
Water and GA3 soaking produced maximum germination of 90.45% in Bharat.
- Material
- Bharate1
- Treatment
- soaked in distilled water along with 2 mg/l GA3 for two dayse0
- Outcome
- germinatione0
- Direction
- maximume0
- Value
- 90.45e0
CapsicumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“The seed soaked in distilled water along with 2 mg/l GA3 for two days prior to sowing had more pronounced effect on both capsicum hybrids recording maximum germination (90.45 and 84.59 %, respectively) in minimum number of days (9.67 days and 10.33 days, respectively).”
e1“Capsicum F1 hybrids Bharat and Indra”
Claim · model-written summary
Water and GA3 soaking produced maximum germination of 84.59% in Indra.
- Material
- Indrae1
- Treatment
- soaked in distilled water along with 2 mg/l GA3 for two dayse0
- Outcome
- germinatione0
- Direction
- maximume0
- Value
- 84.59 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“The seed soaked in distilled water along with 2 mg/l GA3 for two days prior to sowing had more pronounced effect on both capsicum hybrids recording maximum germination (90.45 and 84.59 %, respectively) in minimum number of days (9.67 days and 10.33 days, respectively).”
e1“Capsicum F1 hybrids Bharat and Indra”
Claim · model-written summary
The soaking treatment minimized days to germination in Bharat to 9.67 days.
- Material
- Bharate1
- Treatment
- soaked in distilled water along with 2 mg/l GA3 for two dayse0
- Outcome
- number of dayse0
- Direction
- minimume0
- Value
- 9.67 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“The seed soaked in distilled water along with 2 mg/l GA3 for two days prior to sowing had more pronounced effect on both capsicum hybrids recording maximum germination (90.45 and 84.59 %, respectively) in minimum number of days (9.67 days and 10.33 days, respectively).”
e1“Capsicum F1 hybrids Bharat and Indra”
Claim · model-written summary
The soaking treatment minimized days to germination in Indra to 10.33 days.
- Material
- Indrae1
- Treatment
- soaked in distilled water along with 2 mg/l GA3 for two dayse0
- Outcome
- number of dayse0
- Direction
- minimume0
- Value
- 10.33 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“The seed soaked in distilled water along with 2 mg/l GA3 for two days prior to sowing had more pronounced effect on both capsicum hybrids recording maximum germination (90.45 and 84.59 %, respectively) in minimum number of days (9.67 days and 10.33 days, respectively).”
e1“Capsicum F1 hybrids Bharat and Indra”
Claim · model-written summary
Tissue culture responses varied by genotype, explant orientation and regulator combination.
- Outcome
- Tissue culture responsese0
- Qualifier
- genotypes, side of explants touching the medium and combinations of growth regulatorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“Tissue culture responses varied with the genotypes, side of explants touching the medium and combinations of growth regulators used.”
Claim · model-written summary
Indra cotyledon explants with abaxial contact and zeatin plus GA3 reached 96.30% regeneration.
- Material
- Indrae0
- Treatment
- 7.5 mg/l zeatin along with 2.0 mg/l GA3e0
- Outcome
- Regeneration per cente0
- Direction
- maximume0
- Value
- 96.30%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“Regeneration per cent (96.30%), number of shoots per explants (4.56) and per cent shoot elongation (82.10%) was maximum in hybrid Indra from cotyledon explant having abaxial side in contact with growing medium supplemented with 7.5 mg/l zeatin along with 2.0 mg/l GA3.”
Claim · model-written summary
Under the same conditions Indra produced 4.56 shoots per explant.
- Material
- Indrae0
- Treatment
- 7.5 mg/l zeatin along with 2.0 mg/l GA3e0
- Outcome
- number of shoots per explantse0
- Direction
- maximume0
- Value
- 4.56e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“Regeneration per cent (96.30%), number of shoots per explants (4.56) and per cent shoot elongation (82.10%) was maximum in hybrid Indra from cotyledon explant having abaxial side in contact with growing medium supplemented with 7.5 mg/l zeatin along with 2.0 mg/l GA3.”
Claim · model-written summary
Under the same conditions Indra reached 82.10% shoot elongation.
- Material
- Indrae0
- Treatment
- 7.5 mg/l zeatin along with 2.0 mg/l GA3e0
- Outcome
- per cent shoot elongatione0
- Direction
- maximume0
- Value
- 82.10%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“Regeneration per cent (96.30%), number of shoots per explants (4.56) and per cent shoot elongation (82.10%) was maximum in hybrid Indra from cotyledon explant having abaxial side in contact with growing medium supplemented with 7.5 mg/l zeatin along with 2.0 mg/l GA3.”
Claim · model-written summary
IBA medium produced 100% rooting in both hybrids.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“When regenerated shoots cultured on MS media supplemented with 0.5 mg/l IBA produced 100% rooting in both the hybrids.”
Claim · model-written summary
Survival in hardening was higher in Indra at 92.31% than Bharat at 85.71%.
- Material
- Indrae0
- Outcome
- survival percentagee0
- Direction
- highere0
- Value
- 92.31%e0
- Qualifier
- 85.71%e0
- Comparator
- Bharate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Capsicum (Capsicum annuum L.)”
e0“The survival percentage of regenerated plantlets during hardening in pots containing sterile mixture of 1:1 coco-peat and vermiculite was higher in Indra (92.31%) as compared to Bharat (85.71%).”
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