Abstract
The ascorbate content declined rapidly in broccoli (Brassica oleracea L. var. italica) florets, but not in the stem tissue, during post-harvest senescence. Ascorbate peroxidase (APX), ascorbate oxidase (AO), l-galactono-1,4-lactone dehydrogenase (GLDH), monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) were investigated in gene expression after harvest in both florets and the stem tissue of broccoli. Cytosolic gene expressions (BO-APX 1, BO-APX 2, BO-AO, BO-MDAR 2, and BO-GR) were stimulated actively in broccoli florets after harvest. By contrast, it was observed that mRNA levels of chloroplastic APX, BO-sAPX and BO-tbAPX, had decreased by 12 h after harvest in broccoli florets, suggesting that the active oxygen species (AOS) scavenging system in chloroplasts was largely abolished in florets during the early hours of the post-harvest period. In addition, gene expressions in GLDH and other chloroplastic enzymes such as BO-MDAR 1 and BO-DHAR decreased rapidly within 24 h after harvest. Ethylene treatment had no effect on the ascorbate level and the expression of all genes investigated. The expressions of BO-GLDH and chloroplastic genes (BO-sAPX, BO-tbAPX, BO-MDAR 1, and BO-DHAR) mRNA were suppressed by treatment with methyl jasmonate (MJ) and abscisic acid (ABA) and were accompanied by the acceleration of ascorbate degradation. These data suggest that ascorbate metabolism tends to be inactivated in chloroplasts by transcriptional regulation, but not in the cytosol, when ascorbate decreases under stress conditions.
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Claims 11
Claim · model-written summary
Ascorbate declined rapidly in broccoli florets during postharvest senescence.
- Taxon
- broccoli
Brassica oleracea var. italicae0 - Organ / stage
- floretse0
- Outcome
- ascorbate contente0
- Direction
- declined rapidlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ascorbate content declined rapidly in broccoli (Brassica oleracea L. var. italica) florets”
Claim · model-written summary
Ascorbate did not decline in broccoli stem tissue during postharvest senescence.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Organ / stage
- stem tissuee0
- Outcome
- ascorbate contente2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not in the stem tissue, during post-harvest senescence”
e1“broccoli (Brassica oleracea L. var. italica) florets”
e2“ascorbate content declined rapidly in broccoli (Brassica oleracea L. var. italica) florets”
Claim · model-written summary
Five cytosolic ascorbate-related genes were stimulated after harvest in broccoli florets.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Organ / stage
- floretse0
- Outcome
- Cytosolic gene expressionse0
- Direction
- stimulated activelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytosolic gene expressions (BO-APX 1, BO-APX 2, BO-AO, BO-MDAR 2, and BO-GR) were stimulated actively in broccoli florets after harvest”
Claim · model-written summary
Chloroplastic APX mRNA decreased by 12 hours after harvest.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Gene
- BO-sAPX and BO-tbAPXe0
- Outcome
- mRNA levelse0
- Direction
- decreasede0
- Value
- 12 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA levels of chloroplastic APX, BO-sAPX and BO-tbAPX, had decreased by 12 h after harvest in broccoli florets”
Claim · model-written summary
GLDH, BO-MDAR 1 and BO-DHAR expression declined within 24 hours of harvest.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Outcome
- gene expressionse0
- Direction
- decreased rapidlye0
- Value
- 24 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gene expressions in GLDH and other chloroplastic enzymes such as BO-MDAR 1 and BO-DHAR decreased rapidly within 24 h after harvest”
e1“Ascorbate metabolism in harvested broccoli”
Claim · model-written summary
Ethylene treatment had no effect on broccoli ascorbate level.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Treatment
- Ethylene treatmente0
- Outcome
- ascorbate levele0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ethylene treatment had no effect on the ascorbate level”
e1“Ascorbate metabolism in harvested broccoli”
Claim · model-written summary
Ethylene treatment had no effect on expression of the investigated genes.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Treatment
- Ethylene treatmente0
- Outcome
- expression of all genes investigatede0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ethylene treatment had no effect on the ascorbate level and the expression of all genes investigated”
e1“Ascorbate metabolism in harvested broccoli”
Claim · model-written summary
Methyl jasmonate suppressed BO-GLDH and chloroplastic gene mRNA expression.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Treatment
- methyl jasmonate (MJ)e0
- Outcome
- expressions of BO-GLDH and chloroplastic genese0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expressions of BO-GLDH and chloroplastic genes (BO-sAPX, BO-tbAPX, BO-MDAR 1, and BO-DHAR) mRNA were suppressed by treatment with methyl jasmonate (MJ) and abscisic acid (ABA)”
e1“Ascorbate metabolism in harvested broccoli”
Claim · model-written summary
Abscisic acid suppressed BO-GLDH and chloroplastic gene mRNA expression.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Treatment
- abscisic acid (ABA)e0
- Outcome
- expressions of BO-GLDH and chloroplastic genese0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expressions of BO-GLDH and chloroplastic genes (BO-sAPX, BO-tbAPX, BO-MDAR 1, and BO-DHAR) mRNA were suppressed by treatment with methyl jasmonate (MJ) and abscisic acid (ABA)”
e1“Ascorbate metabolism in harvested broccoli”
Claim · model-written summary
Methyl jasmonate accelerated ascorbate degradation.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Treatment
- methyl jasmonate (MJ)e0
- Outcome
- ascorbate degradatione0
- Direction
- acceleratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with methyl jasmonate (MJ) and abscisic acid (ABA) and were accompanied by the acceleration of ascorbate degradation”
e1“Ascorbate metabolism in harvested broccoli”
Claim · model-written summary
Abscisic acid accelerated ascorbate degradation.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Treatment
- abscisic acid (ABA)e0
- Outcome
- ascorbate degradatione0
- Direction
- acceleratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with methyl jasmonate (MJ) and abscisic acid (ABA) and were accompanied by the acceleration of ascorbate degradation”
e1“Ascorbate metabolism in harvested broccoli”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.