Abstract
A genomic clone for VR-ACS6, an isozyme of auxin-inducible ACC synthase of mungbean, was isolated, and its promoter activity was examined in transgenic tobacco. The clone contained 1,612 bp long 5' untranscribed region and its coding sequence consisted of three exons and two introns. Genomic Southern hybridization indicated that VR-ACS6 is a single copy gene. The transcription initiation site was a cytosine present at 231-base upstream the translation start codon. The VR-ACS6 promoter contained DNA sequences homologous to various functionally identified auxin-responsive elements. To demonstrate hormonal response of the promoter region, transgenic tobacco plants carrying the 1,719 bp VR-ACS6 promoter/-glucuronidase (GUS) fusion gene were generated. Strong GUS expression occurred by auxin treatment of leaves of T0 transformants and hypocotyls of T1 etiolated seedlings. Magnitude of the response to auxin was dose-dependent, and the increased GUS activity was detected at 0.1 microM and higher concentrations of IAA. Other plant hormones did not induce GUS activity, but greatly modified the response to auxin. Cytokinin enhanced the IAA-induced expression of GUS reporter gene, whereas ABA and ethylene suppressed the expression. These characteristics of VR-ACS6 promoter activity in transgenic tobacco are in good accordance with the expression patterns of the gene in mungbean hypocotyls. Histochemical staining showed that GUS activity was evident in both etiolated and light grown seedings treated with IAA. Cytokinin enhanced the intensity of auxin-induced GUS stain and also expanded the stained area, whereas ABA and ethylene reduced both intensity and area of the stain.
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Claims 18
Claim · model-written summary
A genomic VR-ACS6 clone was isolated.
- Taxon
- mungbean
.jpg?width=60)
Vigna radiatae0 - Gene
- VR-ACS6e0
- Outcome
- genomic clonee0
- Value
- VR-ACS6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“A genomic clone for VR-ACS6, an isozyme of auxin-inducible ACC synthase of mungbean, was isolated”
Claim · model-written summary
The clone contained a 1,612 bp 5-prime untranscribed region.
- Taxon
- MungbeanVigna radiatat0
- Outcome
- 5' untranscribed regione0
- Value
- 1,612 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The clone contained 1,612 bp long 5' untranscribed region and its coding sequence consisted of three exons and two introns.”
t0“Mungbean”
Claim · model-written summary
The coding sequence consisted of three exons and two introns.
- Taxon
- MungbeanVigna radiatat0
- Outcome
- coding sequencee0
- Value
- three exons and two intronse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its coding sequence consisted of three exons and two introns.”
t0“Mungbean”
Claim · model-written summary
VR-ACS6 was a single-copy gene.
- Taxon
- MungbeanVigna radiatat0
- Gene
- VR-ACS6e0
- Outcome
- single copy genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genomic Southern hybridization indicated that VR-ACS6 is a single copy gene.”
t0“Mungbean”
Claim · model-written summary
The transcription start site was cytosine 231 bases upstream of the translation start.
- Taxon
- MungbeanVigna radiatat0
- Outcome
- transcription initiation sitee0
- Value
- 231-basee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcription initiation site was a cytosine present at 231-base upstream the translation start codon.”
t0“Mungbean”
Claim · model-written summary
The VR-ACS6 promoter contained homologs of auxin-responsive elements.
- Taxon
- MungbeanVigna radiatat0
- Gene
- VR-ACS6e0
- Outcome
- DNA sequences homologous to various functionally identified auxin-responsive elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The VR-ACS6 promoter contained DNA sequences homologous to various functionally identified auxin-responsive elements.”
t0“Mungbean”
Claim · model-written summary
Auxin strongly induced GUS expression in leaves of T0 tobacco transformants.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Organ / stage
- leaves of T0 transformantse0
- Treatment
- auxin treatmente0
- Outcome
- GUS expressione0
- Direction
- Stronge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strong GUS expression occurred by auxin treatment of leaves of T0 transformants and hypocotyls of T1 etiolated seedlings.”
t0“Transgenic Tobacco”
Claim · model-written summary
Auxin strongly induced GUS expression in hypocotyls of T1 tobacco seedlings.
- Taxon
- TobaccoNicotiana tabacumt0
- Organ / stage
- hypocotyls of T1 etiolated seedlingse0
- Treatment
- auxin treatmente0
- Outcome
- GUS expressione0
- Direction
- Stronge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strong GUS expression occurred by auxin treatment of leaves of T0 transformants and hypocotyls of T1 etiolated seedlings.”
t0“Transgenic Tobacco”
Claim · model-written summary
The auxin response was dose dependent, with GUS activity increased at 0.1 microM IAA and above.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- IAAe0
- Outcome
- GUS activitye0
- Direction
- increasede0
- Value
- 0.1 microMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Magnitude of the response to auxin was dose-dependent, and the increased GUS activity was detected at 0.1 microM and higher concentrations of IAA.”
t0“Transgenic Tobacco”
Claim · model-written summary
Other plant hormones did not induce GUS activity alone.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Other plant hormonese0
- Outcome
- GUS activitye0
- Direction
- did not inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other plant hormones did not induce GUS activity, but greatly modified the response to auxin.”
t0“Transgenic Tobacco”
Claim · model-written summary
Cytokinin enhanced IAA-induced GUS expression.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Cytokinine0
- Outcome
- IAA-induced expression of GUS reporter genee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytokinin enhanced the IAA-induced expression of GUS reporter gene”
t0“Transgenic Tobacco”
Claim · model-written summary
ABA suppressed IAA-induced GUS expression.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- ABAe0
- Outcome
- expressione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA and ethylene suppressed the expression.”
t0“Transgenic Tobacco”
Claim · model-written summary
Ethylene suppressed IAA-induced GUS expression.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- ethylenee0
- Outcome
- expressione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA and ethylene suppressed the expression.”
t0“Transgenic Tobacco”
Claim · model-written summary
Tobacco promoter activity agreed with mungbean hypocotyl gene expression patterns.
- Taxon
- transgenic tobaccoNicotiana tabacume0
- Gene
- VR-ACS6e0
- Outcome
- promoter activitye0
- Direction
- in good accordancee0
- Comparator
- expression patterns of the gene in mungbean hypocotylse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These characteristics of VR-ACS6 promoter activity in transgenic tobacco are in good accordance with the expression patterns of the gene in mungbean hypocotyls.”
Claim · model-written summary
IAA-treated etiolated and light-grown seedlings showed GUS activity.
- Taxon
- TobaccoNicotiana tabacumt0
- Organ / stage
- etiolated and light grown seedingse0
- Treatment
- IAAe0
- Outcome
- GUS activity was evidente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histochemical staining showed that GUS activity was evident in both etiolated and light grown seedings treated with IAA.”
t0“Transgenic Tobacco”
Claim · model-written summary
Cytokinin increased auxin-induced GUS stain intensity and area.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Cytokinine0
- Outcome
- intensity of auxin-induced GUS stain and also expanded the stained areae0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytokinin enhanced the intensity of auxin-induced GUS stain and also expanded the stained area”
t0“Transgenic Tobacco”
Claim · model-written summary
ABA reduced intensity and area of auxin-induced GUS staining.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- ABAe0
- Outcome
- intensity and area of the staine0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA and ethylene reduced both intensity and area of the stain.”
t0“Transgenic Tobacco”
Claim · model-written summary
Ethylene reduced intensity and area of auxin-induced GUS staining.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- ethylenee0
- Outcome
- intensity and area of the staine0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA and ethylene reduced both intensity and area of the stain.”
t0“Transgenic Tobacco”
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