Abstract
We describe here the results of a comparison of the properties of several plant genotypes differing in their reaction to auxins. The hormonal response used to compare the genotypes is the auxin-induced variation of the transplasmalemma electrical potential difference (delta Em) exhibited by protoplasts isolated from leaves or root tips. Using this membrane response, we have shown that large variations in the sensitivity to auxins can be induced either by mutagenesis in tobacco or by transformation of various materials by Agrobacterium rhizogenes. Tobacco protoplasts isolated from an auxin resistant mutant selected by M. Caboche (INRA, Versailles), when compared to protoplasts from the control genotype display an auxin-induced hyperpolarization with a 10 fold decrease in their sensitivity to the hormone. Conversely, protoplasts isolated from root tips of various plants transformed by A. rhizogenes or from the leaves of tobacco plants regenerated from transformed roots, exhibit a 100 to 1000 fold increase in their sensitivity to auxins. The single gene, rol B, from the Ri plasmid harboured by A. rhizogenes is able to induce the dramatic modification in the sensitivity to auxins of tobacco protoplasts. The auxin-induced hyperpolarization of tobacco protoplasts is inhibited by antibodies directed against an auxin-binding protein (ABP) purified from corn coleoptile membranes. Immunotitrations of ABP antigens at the external surface of tobacco protoplasts show that transformed protoplasts could have more surface receptors than protoplasts from the normal genotype, whereas protoplasts isolated from the auxin-tolerant mutant could have less receptors than their wild-type counterpart. These results suggest that the sensitivity of tobacco protoplasts to auxin could be dependent on the number of functional receptors exposed at the outer surface of the plasmalemma.
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Claims 8
Claim · model-written summary
A tobacco auxin-resistant mutant showed a tenfold decrease in auxin sensitivity compared with control.
- Taxon
- Tobacco
Nicotiana tabacume0 - Material
- auxin resistant mutante0
- Organ / stage
- protoplastse0
- Outcome
- sensitivity to the hormonee0
- Direction
- decreasee0
- Value
- 10 folde0
- Comparator
- control genotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tobacco protoplasts isolated from an auxin resistant mutant selected by M. Caboche (INRA, Versailles), when compared to protoplasts from the control genotype display an auxin-induced hyperpolarization with a 10 fold decrease in their sensitivity to the hormone.”
Claim · model-written summary
Root-tip protoplasts of plants transformed by A. rhizogenes had 100 to 1000 fold greater auxin sensitivity.
- Taxon
- various plantse0
- Organ / stage
- root tipse0
- Treatment
- transformed by A. rhizogenese0
- Interactor
- A. rhizogenese0other
- Outcome
- sensitivity to auxinse0
- Direction
- increasee0
- Value
- 100 to 1000 folde0
Not reportedComparator
Verbatim
e0“protoplasts isolated from root tips of various plants transformed by A. rhizogenes or from the leaves of tobacco plants regenerated from transformed roots, exhibit a 100 to 1000 fold increase in their sensitivity to auxins.”
Claim · model-written summary
Leaf protoplasts from tobacco regenerated from transformed roots had 100 to 1000 fold greater auxin sensitivity.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leavese0
- Treatment
- regenerated from transformed rootse0
- Outcome
- sensitivity to auxinse0
- Direction
- increasee0
- Value
- 100 to 1000 folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protoplasts isolated from root tips of various plants transformed by A. rhizogenes or from the leaves of tobacco plants regenerated from transformed roots, exhibit a 100 to 1000 fold increase in their sensitivity to auxins.”
Claim · model-written summary
The rol B gene induced altered auxin sensitivity in tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- A. rhizogenese0other
- Gene
- rol Be0
- Outcome
- sensitivity to auxinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The single gene, rol B, from the Ri plasmid harboured by A. rhizogenes is able to induce the dramatic modification in the sensitivity to auxins of tobacco protoplasts.”
Claim · model-written summary
Antibodies against ABP inhibited auxin-induced hyperpolarization of tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- protoplastse0
- Treatment
- antibodies directed against an auxin-binding protein (ABP)e0
- Gene
- auxin-binding protein (ABP)e0
- Outcome
- auxin-induced hyperpolarizatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The auxin-induced hyperpolarization of tobacco protoplasts is inhibited by antibodies directed against an auxin-binding protein (ABP) purified from corn coleoptile membranes.”
Claim · model-written summary
Transformed tobacco protoplasts could have more surface receptors than normal protoplasts.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- surface receptorse0
- Direction
- moree0
- Comparator
- normal genotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transformed protoplasts could have more surface receptors than protoplasts from the normal genotype”
e1“Tobacco protoplasts isolated from an auxin resistant mutant selected by M. Caboche (INRA, Versailles), when compared to protoplasts from the control genotype display an auxin-induced hyperpolarization with a 10 fold decrease in their sensitivity to the hormone.”
Claim · model-written summary
Auxin-tolerant mutant protoplasts could have fewer receptors than wild type.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- auxin-tolerant mutante0
- Outcome
- receptorse0
- Direction
- lesse0
- Comparator
- wild-type counterparte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protoplasts isolated from the auxin-tolerant mutant could have less receptors than their wild-type counterpart”
e1“Tobacco protoplasts isolated from an auxin resistant mutant selected by M. Caboche (INRA, Versailles), when compared to protoplasts from the control genotype display an auxin-induced hyperpolarization with a 10 fold decrease in their sensitivity to the hormone.”
Claim · model-written summary
Tobacco protoplast auxin sensitivity may depend on the number of functional outer plasmalemma receptors.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- sensitivity of tobacco protoplasts to auxine0
- Qualifier
- number of functional receptors exposed at the outer surface of the plasmalemmae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the sensitivity of tobacco protoplasts to auxin could be dependent on the number of functional receptors exposed at the outer surface of the plasmalemma.”
The same questions in other papers
class · physiology: hormones · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.