Abstract
Glucosinolates are a group of secondary metabolites that function as defense substances against herbivores and micro-organisms in the plant order Capparales. Indole glucosinolates (IGS), derivatives of tryptophan, may also influence plant growth and development. In Arabidopsis thaliana, indole-3-acetaldoxime (IAOx) produced from tryptophan by the activity of two cytochrome P450 enzymes, CYP79B2 and CYP79B3, serves as a precursor for IGS biosynthesis but is also an intermediate in the biosynthetic pathway of indole-3-acetic acid (IAA). Another cytochrome P450 enzyme, CYP83B1, funnels IAOx into IGS. Although there is increasing information about the genes involved in this biochemical pathway, their regulation is not fully understood. OBP2 has recently been identified as a member of the DNA-binding-with-one-finger (DOF) transcription factors, but its function has not been studied in detail so far. Here we report that OBP2 is expressed in the vasculature of all Arabidopsis organs, including leaves, roots, flower stalks and petals. OBP2 expression is induced in response to a generalist herbivore, Spodoptera littoralis, and by treatment with the plant signalling molecule methyl jasmonate, both of which also trigger IGS accumulation. Constitutive and inducible over-expression of OBP2 activates expression of CYP83B1. In addition, auxin concentration is increased in leaves and seedlings of OBP2 over-expression lines relative to wild-type, and plant size is diminished due to a reduction in cell size. RNA interference-mediated OBP2 blockade leads to reduced expression of CYP83B1. Collectively, these data provide evidence that OBP2 is part of a regulatory network that regulates glucosinolate biosynthesis in Arabidopsis.
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Claims 10
Claim · model-written summary
OBP2 was expressed in vasculature across Arabidopsis organs.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- vasculaturee0
- Gene
- OBP2e0
- Outcome
- expressed in the vasculature of all Arabidopsis organse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OBP2 is expressed in the vasculature of all Arabidopsis organs, including leaves, roots, flower stalks and petals”
Claim · model-written summary
Herbivory by Spodoptera littoralis induced OBP2 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Spodoptera littoralise0
- Interactor
- Spodoptera littoralis
Spodoptera littoralise0herbivore - Gene
- OBP2e0
- Outcome
- OBP2 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OBP2 expression is induced in response to a generalist herbivore, Spodoptera littoralis”
t0“Arabidopsis”
Claim · model-written summary
Methyl jasmonate induced OBP2 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- treatment with the plant signalling molecule methyl jasmonatee0
- Gene
- OBP2e0
- Outcome
- OBP2 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OBP2 expression is induced in response to a generalist herbivore, Spodoptera littoralis, and by treatment with the plant signalling molecule methyl jasmonate”
t0“Arabidopsis”
Claim · model-written summary
Spodoptera littoralis herbivory triggered IGS accumulation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Spodoptera littoralise0
- Interactor
- Spodoptera littoralisSpodoptera littoralise0herbivore
- Outcome
- IGS accumulatione0
- Direction
- triggere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OBP2 expression is induced in response to a generalist herbivore, Spodoptera littoralis, and by treatment with the plant signalling molecule methyl jasmonate, both of which also trigger IGS accumulation”
t0“Arabidopsis”
Claim · model-written summary
Methyl jasmonate triggered IGS accumulation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- treatment with the plant signalling molecule methyl jasmonatee0
- Outcome
- IGS accumulatione0
- Direction
- triggere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with the plant signalling molecule methyl jasmonate, both of which also trigger IGS accumulation”
t0“Arabidopsis”
Claim · model-written summary
Constitutive OBP2 overexpression activated CYP83B1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Constitutivee0
- Gene
- CYP83B1e0
- Outcome
- expression of CYP83B1e0
- Direction
- activatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Constitutive and inducible over-expression of OBP2 activates expression of CYP83B1”
t0“Arabidopsis”
Claim · model-written summary
Inducible OBP2 overexpression activated CYP83B1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- inducible over-expression of OBP2e0
- Gene
- CYP83B1e0
- Outcome
- expression of CYP83B1e0
- Direction
- activatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Constitutive and inducible over-expression of OBP2 activates expression of CYP83B1”
t0“Arabidopsis”
Claim · model-written summary
OBP2 overexpression raised auxin concentration in leaves and seedlings relative to wild type.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- leaves and seedlingse0
- Treatment
- OBP2 over-expression linese0
- Outcome
- auxin concentratione0
- Direction
- increasede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin concentration is increased in leaves and seedlings of OBP2 over-expression lines relative to wild-type”
t0“Arabidopsis”
Claim · model-written summary
OBP2 overexpression reduced plant size through reduced cell size.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- OBP2 over-expression linese1
- Outcome
- plant sizee0
- Direction
- diminishede0
- Qualifier
- reduction in cell sizee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant size is diminished due to a reduction in cell size”
e1“OBP2 over-expression lines”
t0“Arabidopsis”
Claim · model-written summary
RNA interference blockade of OBP2 reduced CYP83B1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- RNA interference-mediated OBP2 blockadee0
- Gene
- CYP83B1e0
- Outcome
- expression of CYP83B1e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference-mediated OBP2 blockade leads to reduced expression of CYP83B1”
t0“Arabidopsis”
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