Abstract
In Arabidopsis, the rhizobacterial strain Pseudomonas fluorescens WCS417r triggers jasmonate (JA)- and ethylene (ET)-dependent induced systemic resistance (ISR) that is effective against different pathogens. Arabidopsis genotypes unable to express rhizobacteria-mediated ISR against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) exhibit enhanced disease susceptibility towards this pathogen. To identify novel components controlling induced resistance, we tested 11 Arabidopsis mutants with enhanced disease susceptibility (eds) to pathogenic P. syringae bacteria for WCS417r-mediated ISR and pathogen-induced systemic acquired resistance (SAR). Mutants eds4-1, eds8-1 and eds10-1 failed to develop WCS417r-mediated ISR, while mutants eds5-1 and eds12-1 failed to express pathogen-induced SAR. Whereas eds5-1 is known to be blocked in salicylic acid (SA) biosynthesis, analysis of eds12-1 revealed that its impaired SAR response is caused by reduced sensitivity to this molecule. Analysis of the ISR-impaired eds mutants revealed that they are non-responsive to induction of resistance by methyl jasmonate (MeJA) (eds4-1, eds8-1 and eds10-1), or the ET precursor 1-aminocyclopropane-1-carboxylate (ACC) (eds4-1 and eds10-1). Moreover, eds4-1 and eds8-1 showed reduced expression of the plant defensin gene PDF1.2 after MeJA and ACC treatment, which was associated with reduced sensitivity to either ET (eds4-1) or MeJA (eds8-1). Although blocked in WCS417r-, MeJA- and ACC-induced ISR, eds10-1 behaved normally for several other responses to MeJA or ACC. The results indicate that EDS12 is required for SAR and acts downstream of SA, whereas EDS4, EDS8 and EDS10 are required for ISR acting either in JA signalling (EDS8), ET signalling (EDS4), or downstream JA and ET signalling (EDS10) in the ISR pathway.
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Claims 20
Claim · model-written summary
eds4-1 failed to develop WCS417r-mediated ISR.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- eds4-1e0
- Interactor
- WCS417re0
- Outcome
- failed to develop WCS417r-mediated ISRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutants eds4-1, eds8-1 and eds10-1 failed to develop WCS417r-mediated ISR, while mutants eds5-1 and eds12-1 failed to express pathogen-induced SAR.”
t0“Arabidopsis”
Claim · model-written summary
eds8-1 failed to develop WCS417r-mediated ISR.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds8-1e0
- Interactor
- WCS417re0
- Outcome
- failed to develop WCS417r-mediated ISRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutants eds4-1, eds8-1 and eds10-1 failed to develop WCS417r-mediated ISR, while mutants eds5-1 and eds12-1 failed to express pathogen-induced SAR.”
t0“Arabidopsis”
Claim · model-written summary
eds10-1 failed to develop WCS417r-mediated ISR.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds10-1e0
- Interactor
- WCS417re0
- Outcome
- failed to develop WCS417r-mediated ISRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutants eds4-1, eds8-1 and eds10-1 failed to develop WCS417r-mediated ISR, while mutants eds5-1 and eds12-1 failed to express pathogen-induced SAR.”
t0“Arabidopsis”
Claim · model-written summary
eds5-1 failed to express pathogen-induced SAR.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds5-1e0
- Outcome
- failed to express pathogen-induced SARe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutants eds4-1, eds8-1 and eds10-1 failed to develop WCS417r-mediated ISR, while mutants eds5-1 and eds12-1 failed to express pathogen-induced SAR.”
t0“Arabidopsis”
Claim · model-written summary
eds12-1 failed to express pathogen-induced SAR.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds12-1e0
- Outcome
- failed to express pathogen-induced SARe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutants eds4-1, eds8-1 and eds10-1 failed to develop WCS417r-mediated ISR, while mutants eds5-1 and eds12-1 failed to express pathogen-induced SAR.”
t0“Arabidopsis”
Claim · model-written summary
Reduced sensitivity to salicylic acid was reported to cause impaired SAR in eds12-1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds12-1e0
- Outcome
- impaired SAR responsee0
- Qualifier
- reduced sensitivity to this moleculee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analysis of eds12-1 revealed that its impaired SAR response is caused by reduced sensitivity to this molecule.”
t0“Arabidopsis”
Claim · model-written summary
eds4-1 did not respond to methyl jasmonate induction of resistance.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds4-1e0
- Treatment
- methyl jasmonate (MeJA)e0
- Outcome
- induction of resistancee0
- Direction
- non-responsivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the ISR-impaired eds mutants revealed that they are non-responsive to induction of resistance by methyl jasmonate (MeJA) (eds4-1, eds8-1 and eds10-1), or the ET precursor 1-aminocyclopropane-1-carboxylate (ACC) (eds4-1 and eds10-1).”
t0“Arabidopsis”
Claim · model-written summary
eds8-1 did not respond to methyl jasmonate induction of resistance.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds8-1e0
- Treatment
- methyl jasmonate (MeJA)e0
- Outcome
- induction of resistancee0
- Direction
- non-responsivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the ISR-impaired eds mutants revealed that they are non-responsive to induction of resistance by methyl jasmonate (MeJA) (eds4-1, eds8-1 and eds10-1), or the ET precursor 1-aminocyclopropane-1-carboxylate (ACC) (eds4-1 and eds10-1).”
t0“Arabidopsis”
Claim · model-written summary
eds10-1 did not respond to methyl jasmonate induction of resistance.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds10-1e0
- Treatment
- methyl jasmonate (MeJA)e0
- Outcome
- induction of resistancee0
- Direction
- non-responsivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the ISR-impaired eds mutants revealed that they are non-responsive to induction of resistance by methyl jasmonate (MeJA) (eds4-1, eds8-1 and eds10-1), or the ET precursor 1-aminocyclopropane-1-carboxylate (ACC) (eds4-1 and eds10-1).”
t0“Arabidopsis”
Claim · model-written summary
eds4-1 did not respond to ACC induction of resistance.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds4-1e0
- Treatment
- 1-aminocyclopropane-1-carboxylate (ACC)e0
- Outcome
- induction of resistancee0
- Direction
- non-responsivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the ISR-impaired eds mutants revealed that they are non-responsive to induction of resistance by methyl jasmonate (MeJA) (eds4-1, eds8-1 and eds10-1), or the ET precursor 1-aminocyclopropane-1-carboxylate (ACC) (eds4-1 and eds10-1).”
t0“Arabidopsis”
Claim · model-written summary
eds10-1 did not respond to ACC induction of resistance.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds10-1e0
- Treatment
- 1-aminocyclopropane-1-carboxylate (ACC)e0
- Outcome
- induction of resistancee0
- Direction
- non-responsivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the ISR-impaired eds mutants revealed that they are non-responsive to induction of resistance by methyl jasmonate (MeJA) (eds4-1, eds8-1 and eds10-1), or the ET precursor 1-aminocyclopropane-1-carboxylate (ACC) (eds4-1 and eds10-1).”
t0“Arabidopsis”
Claim · model-written summary
eds4-1 showed reduced PDF1.2 expression after MeJA and ACC treatment.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds4-1e0
- Treatment
- MeJA and ACC treatmente0
- Gene
- PDF1.2e0
- Outcome
- expression of the plant defensin gene PDF1.2e0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, eds4-1 and eds8-1 showed reduced expression of the plant defensin gene PDF1.2 after MeJA and ACC treatment, which was associated with reduced sensitivity to either ET (eds4-1) or MeJA (eds8-1).”
t0“Arabidopsis”
Claim · model-written summary
eds8-1 showed reduced PDF1.2 expression after MeJA and ACC treatment.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds8-1e0
- Treatment
- MeJA and ACC treatmente0
- Gene
- PDF1.2e0
- Outcome
- expression of the plant defensin gene PDF1.2e0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, eds4-1 and eds8-1 showed reduced expression of the plant defensin gene PDF1.2 after MeJA and ACC treatment, which was associated with reduced sensitivity to either ET (eds4-1) or MeJA (eds8-1).”
t0“Arabidopsis”
Claim · model-written summary
eds4-1 had reduced sensitivity to ET.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, eds4-1 and eds8-1 showed reduced expression of the plant defensin gene PDF1.2 after MeJA and ACC treatment, which was associated with reduced sensitivity to either ET (eds4-1) or MeJA (eds8-1).”
t0“Arabidopsis”
Claim · model-written summary
eds8-1 had reduced sensitivity to MeJA.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, eds4-1 and eds8-1 showed reduced expression of the plant defensin gene PDF1.2 after MeJA and ACC treatment, which was associated with reduced sensitivity to either ET (eds4-1) or MeJA (eds8-1).”
t0“Arabidopsis”
Claim · model-written summary
eds10-1 behaved normally for other responses to MeJA or ACC.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eds10-1e0
- Outcome
- behaved normally for several other responses to MeJA or ACCe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although blocked in WCS417r-, MeJA- and ACC-induced ISR, eds10-1 behaved normally for several other responses to MeJA or ACC.”
t0“Arabidopsis”
Claim · model-written summary
EDS12 functions in required for SAR and acts downstream of SA.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- EDS12e0
- Outcome
- required for SAR and acts downstream of SAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results indicate that EDS12 is required for SAR and acts downstream of SA, whereas EDS4, EDS8 and EDS10 are required for ISR acting either in JA signalling (EDS8), ET signalling (EDS4), or downstream JA and ET signalling (EDS10) in the ISR pathway.”
t0“Arabidopsis”
Claim · model-written summary
EDS8 functions in JA signalling.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- EDS8e0
- Outcome
- JA signallinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results indicate that EDS12 is required for SAR and acts downstream of SA, whereas EDS4, EDS8 and EDS10 are required for ISR acting either in JA signalling (EDS8), ET signalling (EDS4), or downstream JA and ET signalling (EDS10) in the ISR pathway.”
t0“Arabidopsis”
Claim · model-written summary
EDS4 functions in ET signalling.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- EDS4e0
- Outcome
- ET signallinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results indicate that EDS12 is required for SAR and acts downstream of SA, whereas EDS4, EDS8 and EDS10 are required for ISR acting either in JA signalling (EDS8), ET signalling (EDS4), or downstream JA and ET signalling (EDS10) in the ISR pathway.”
t0“Arabidopsis”
Claim · model-written summary
EDS10 functions in downstream JA and ET signalling.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- EDS10e0
- Outcome
- downstream JA and ET signallinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results indicate that EDS12 is required for SAR and acts downstream of SA, whereas EDS4, EDS8 and EDS10 are required for ISR acting either in JA signalling (EDS8), ET signalling (EDS4), or downstream JA and ET signalling (EDS10) in the ISR pathway.”
t0“Arabidopsis”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.