Abstract
Plants derive a number of important secondary metabolites from the amino acid tryptophan (Trp), including the growth regulator indole-3-acetic acid (IAA) and defense compounds against pathogens and herbivores. In previous work, we found that a dominant overexpression allele of the Arabidopsis (Arabidopsis thaliana) Myb transcription factor ATR1, atr1D, activates expression of a Trp synthesis gene as well as the Trp-metabolizing genes CYP79B2, CYP79B3, and CYP83B1, which encode enzymes implicated in production of IAA and indolic glucosinolate (IG) antiherbivore compounds. Here, we show that ATR1 overexpression confers elevated levels of IAA and IGs. In addition, we show that an atr1 loss-of-function mutation impairs expression of IG synthesis genes and confers reduced IG levels. Furthermore, the atr1-defective mutation suppresses Trp gene dysregulation in a cyp83B1 mutant background. Together, this work implicates ATR1 as a key homeostatic regulator of Trp metabolism and suggests that ATR1 can be manipulated to coordinately control the suite of enzymes that synthesize IGs.
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Claims 7
Claim · model-written summary
ATR1 overexpression elevated IAA levels.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- ATR1 overexpressione0
- Gene
- ATR1e0
- Outcome
- levels of IAAe0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ATR1 overexpression confers elevated levels of IAA and IGs.”
t0“Arabidopsis”
Claim · model-written summary
ATR1 overexpression elevated indolic glucosinolate levels.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ATR1 overexpressione0
- Gene
- ATR1e0
- Outcome
- IGse0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ATR1 overexpression confers elevated levels of IAA and IGs.”
t0“Arabidopsis”
Claim · model-written summary
The atr1 loss-of-function mutation impaired expression of indolic glucosinolate synthesis genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- atr1 loss-of-function mutatione0
- Gene
- atr1e0
- Outcome
- expression of IG synthesis genese0
- Direction
- impairse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an atr1 loss-of-function mutation impairs expression of IG synthesis genes and confers reduced IG levels.”
t0“Arabidopsis”
Claim · model-written summary
The atr1 loss-of-function mutation reduced indolic glucosinolate levels.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- atr1 loss-of-function mutatione0
- Gene
- atr1e0
- Outcome
- IG levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an atr1 loss-of-function mutation impairs expression of IG synthesis genes and confers reduced IG levels.”
t0“Arabidopsis”
Claim · model-written summary
An atr1-defective mutation suppressed tryptophan gene dysregulation in the cyp83B1 background.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cyp83B1 mutant backgrounde0
- Treatment
- atr1-defective mutatione0
- Gene
- cyp83B1e0
- Outcome
- Trp gene dysregulatione0
- Direction
- suppressese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the atr1-defective mutation suppresses Trp gene dysregulation in a cyp83B1 mutant background.”
t0“Arabidopsis”
Claim · model-written summary
The authors implicate ATR1 as a homeostatic regulator of tryptophan metabolism.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ATR1e0
- Outcome
- Trp metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Together, this work implicates ATR1 as a key homeostatic regulator of Trp metabolism and suggests that ATR1 can be manipulated to coordinately control the suite of enzymes that synthesize IGs.”
t0“Arabidopsis”
Claim · model-written summary
The authors suggest ATR1 manipulation could coordinately control enzymes synthesizing indolic glucosinolates.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ATR1e0
- Outcome
- coordinately control the suite of enzymes that synthesize IGse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggests that ATR1 can be manipulated to coordinately control the suite of enzymes that synthesize IGs.”
t0“Arabidopsis”
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