Abstract
A recently isolated cDNA clone of tobacco (Nicotiana tabacum L.) lipoxygenase (LOX) was used to study LOX gene expression in tobacco cell-suspension cultures and intact plants in response to infection with Phytophthora parasitica nicotianae (Ppn). Southern blot analysis of tobacco DNA indicated that only a small number of LOX genes hybridize to this probe. These genes were not constitutively expressed to a detectable level in control cells and healthy plants. In contrast, a rapid and transient accumulation of transcripts occurred in cells and plants after treatment with elicitor and inoculation with zoospores of Ppn, respectively. In cell cultures LOX gene expression could also be induced by linolenic acid, a LOX substrate, and by methyl jasmonate, one of the products derived from the action of LOX on linolenic acid. In the infection assays, LOX gene expression and enzyme activity were observed earlier when the plants carried a resistance gene against the race of Ppn used for inoculation. The differential expression of LOX during the race-cultivar-specific interaction between tobacco and Ppn, as well as its regulation by elicitors and jasmonate, suggest a role of LOX in plant resistance and establishment of the defense status against this pathogen.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Only a small number of tobacco LOX genes hybridize to the lipoxygenase cDNA probe.
- Taxon
- tobacco (Nicotiana tabacum L.)
Nicotiana tabacume0 - Outcome
- a small number of LOX genese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e2“Southern blot analysis of tobacco DNA indicated that only a small number of LOX genes hybridize to this probe”
Claim · model-written summary
LOX genes are not constitutively expressed to a detectable level in control cells and healthy plants.
- Taxon
- tobacco (Nicotiana tabacum L.)Nicotiana tabacume0
- Outcome
- constitutively expressede3
- Qualifier
- to a detectable level in control cells and healthy plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e3“These genes were not constitutively expressed to a detectable level in control cells and healthy plants”
Claim · model-written summary
Elicitor treatment and zoospore inoculation with Ppn rapidly and transiently increased LOX transcript accumulation compared with control cells and healthy plants.
- Taxon
- tobacco (Nicotiana tabacum L.)Nicotiana tabacume0
- Treatment
- elicitor and inoculation with zoospores of Ppne4
- Interactor
- Phytophthora parasitica nicotianae
Phytophthora nicotianaee1pathogen - Outcome
- accumulation of transcriptse4
- Direction
- increaseinferrede4
- Comparator
- control cells and healthy plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e1“in response to infection with Phytophthora parasitica nicotianae (Ppn)”
e3“These genes were not constitutively expressed to a detectable level in control cells and healthy plants”
e4“a rapid and transient accumulation of transcripts occurred in cells and plants after treatment with elicitor and inoculation with zoospores of Ppn, respectively”
Claim · model-written summary
LOX gene expression and enzyme activity were observed earlier in plants carrying a resistance gene against the race of Ppn used for inoculation.
- Taxon
- tobacco (Nicotiana tabacum L.)Nicotiana tabacume0
- Interactor
- Phytophthora parasitica nicotianaePhytophthora nicotianaee1pathogen
- Gene
- resistance genee5
- Outcome
- LOX gene expression and enzyme activitye5
- Direction
- earliere5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e1“in response to infection with Phytophthora parasitica nicotianae (Ppn)”
e5“LOX gene expression and enzyme activity were observed earlier when the plants carried a resistance gene against the race of Ppn used for inoculation”
Claim · model-written summary
Linolenic acid, a LOX substrate, induced LOX gene expression in tobacco cell cultures.
- Taxon
- tobacco (Nicotiana tabacum L.)Nicotiana tabacume0
- Treatment
- linolenic acide6
- Outcome
- LOX gene expressione6
- Direction
- inducede6
- Setting
- cell culturese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e6“In cell cultures LOX gene expression could also be induced by linolenic acid, a LOX substrate, and by methyl jasmonate, one of the products derived from the action of LOX on linolenic acid”
Claim · model-written summary
Methyl jasmonate induced LOX gene expression in tobacco cell cultures.
- Taxon
- tobacco (Nicotiana tabacum L.)Nicotiana tabacume0
- Treatment
- methyl jasmonatee6
- Outcome
- LOX gene expressione6
- Direction
- inducede6
- Setting
- cell culturese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e6“In cell cultures LOX gene expression could also be induced by linolenic acid, a LOX substrate, and by methyl jasmonate, one of the products derived from the action of LOX on linolenic acid”
Claim · model-written summary
LOX may play a role in plant resistance and in establishing the defense status against Ppn.
- Taxon
- tobacco (Nicotiana tabacum L.)Nicotiana tabacume0
- Gene
- LOXe7
- Outcome
- plant resistancee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e7“suggest a role of LOX in plant resistance and establishment of the defense status against this pathogen”
The same questions in other papers
resistance inducer · Phytophthora nicotianae · Nicotiana tabacum (tobacco)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
5 papers more in this group
See all 9 papers →inoculation · Phytophthora nicotianae · Nicotiana tabacum (tobacco)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.