Abstract
FLP site-specific recombinase was expressed in stably transformed tobacco and Arabidopsis. FLP-expressing tobacco lines were crossed with other transformed tobacco lines that contained a stably integrated FLP recognition target construct(s). The target construct consisted of two directly-oriented FLP recognition targets (FRTs), flanking a hygromycin resistance cassette located between a GUS coding region and an upstream 35S CaMV promoter. Excision of the hygromycin resistance cassette by FLP-mediated recombination between FRTs brings the GUS coding region under the transcriptional control of the CaMV 35S promoter. In the absence of FLP-mediated recombination, the GUS gene is transcriptionally silent. GUS activity was observed in the progeny of all crosses made between FLP recombinase-expressing and target-containing tobacco lines, but not in the selfs of parents. The predicted recombination product remaining after excision was confirmed by PCR and Southern analysis. In Arabidopsis, inducible expression of FLP recombinase was achieved from the soybean Gmhsp 17.6L heat-shock promoter. Heat-shock induction of FLP expression in plants containing the target construct led to activation of constitutive GUS expression in a subset of cells, whose progeny, therefore, were GUS-positive. A variety of clonal sectors were produced in plants derived from seed that was heat-shocked during germination. The ability to control the timing of GUS activation was demonstrated by heat-shock of unopened flower heads which produced large sectors. It was concluded that heat-shock-induced expression of FLP recombinase provides a readily controllable method for generating marked clonal sectors in Arabidopsis, the size and distribution of which reflects the timing of applied heat-shock.
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Claims 8
Claim · model-written summary
GUS activity was observed in progeny of crosses between FLP-expressing and target tobacco lines but not in selfed parents.
- Taxon
- tobacco
Nicotiana tabacume1 - Treatment
- crosses made between FLP recombinase-expressing and target-containing tobacco linese1
- Gene
- GUSe1
- Outcome
- GUS activitye1
- Comparator
- selfs of parentse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FLP site-specific recombinase was expressed in stably transformed tobacco and Arabidopsis”
e1“GUS activity was observed in the progeny of all crosses made between FLP recombinase-expressing and target-containing tobacco lines, but not in the selfs of parents”
Claim · model-written summary
The predicted recombination product after excision was confirmed by PCR and Southern analysis in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- recombination producte1
- Qualifier
- confirmed by PCR and Southern analysise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FLP site-specific recombinase was expressed in stably transformed tobacco and Arabidopsis”
e1“The predicted recombination product remaining after excision was confirmed by PCR and Southern analysis”
Claim · model-written summary
Inducible FLP recombinase expression in Arabidopsis was achieved from a soybean heat-shock promoter.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- FLP recombinasee0
- Outcome
- inducible expression of FLP recombinasee0
- Qualifier
- from the soybean Gmhsp 17.6L heat-shock promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, inducible expression of FLP recombinase was achieved from the soybean Gmhsp 17.6L heat-shock promoter”
Claim · model-written summary
Heat-shock induction of FLP expression led to activation of constitutive GUS expression in a subset of Arabidopsis cells.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- subset of cellse1
- Treatment
- Heat-shock induction of FLP expressione1
- Gene
- GUSe1
- Outcome
- constitutive GUS expressione1
- Direction
- led to activatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, inducible expression of FLP recombinase was achieved from the soybean Gmhsp 17.6L heat-shock promoter”
e1“Heat-shock induction of FLP expression in plants containing the target construct led to activation of constitutive GUS expression in a subset of cells”
Claim · model-written summary
Plants derived from seed heat-shocked during germination produced a variety of clonal sectors.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- clonal sectorse1
- Qualifier
- heat-shocked during germinatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, inducible expression of FLP recombinase was achieved from the soybean Gmhsp 17.6L heat-shock promoter”
e1“A variety of clonal sectors were produced in plants derived from seed that was heat-shocked during germination”
Claim · model-written summary
Heat-shock of unopened flower heads controlled the timing of GUS activation in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- unopened flower headse1
- Gene
- GUSe1
- Outcome
- control the timing of GUS activatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, inducible expression of FLP recombinase was achieved from the soybean Gmhsp 17.6L heat-shock promoter”
e1“The ability to control the timing of GUS activation was demonstrated by heat-shock of unopened flower heads”
Claim · model-written summary
Heat-shock of unopened flower heads produced large clonal sectors in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- heat-shock of unopened flower headse1
- Outcome
- large sectorse1
- Direction
- producede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, inducible expression of FLP recombinase was achieved from the soybean Gmhsp 17.6L heat-shock promoter”
e1“heat-shock of unopened flower heads which produced large sectors”
Claim · model-written summary
Heat-shock-induced FLP expression is a readily controllable method for generating marked clonal sectors whose size and distribution reflect the timing of heat-shock in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- FLP recombinasee1
- Outcome
- size and distributione1
- Qualifier
- reflects the timing of applied heat-shocke1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, inducible expression of FLP recombinase was achieved from the soybean Gmhsp 17.6L heat-shock promoter”
e1“It was concluded that heat-shock-induced expression of FLP recombinase provides a readily controllable method for generating marked clonal sectors in Arabidopsis, the size and distribution of which reflects the timing of applied heat-shock”
The same questions in other papers
heat · molecular: expression · ArabidopsisPositive and negative results
10 papers in the same direction as this paper · 18 in the opposite direction
- negative
- no effect
- no effect
25 papers more in this group
See all 29 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.