Abstract
ZPT2-related proteins that have two canonical Cys-2/His-2-type zinc-finger motifs in their molecules are members of a family of plant transcription factors. To characterize the role of this type of protein, we analyzed the function of Arabidopsis L. Heynh. genes encoding four different ZPT2-related proteins (AZF1, AZF2, AZF3, and STZ). Gel-shift analysis showed that the AZFs and STZ bind to A(G/C)T repeats within an EP2 sequence, known as a target sequence of some petunia (Petunia hybrida) ZPT2 proteins. Transient expression analysis using synthetic green fluorescent protein fusion genes indicated that the AZFs and STZ are preferentially localized to the nucleus. These four ZPT2-related proteins were shown to act as transcriptional repressors that down-regulate the transactivation activity of other transcription factors. RNA gel-blot analysis showed that expression of AZF2 and STZ was strongly induced by dehydration, high-salt and cold stresses, and abscisic acid treatment. Histochemical analysis of beta-glucuronidase activities driven by the AZF2 or STZ promoters revealed that both genes are induced in leaves rather than roots of rosette plants by the stresses. Transgenic Arabidopsis overexpressing STZ showed growth retardation and tolerance to drought stress. These results suggest that AZF2 and STZ function as transcriptional repressors to increase stress tolerance following growth retardation.
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Claims 11
Claim · model-written summary
AZFs and STZ bind A(G/C)T repeats in EP2.
- Taxon
- Arabidopsis
Arabidopsise1 - Gene
- AZFs and STZe0
- Outcome
- bind to A(G/C)T repeats within an EP2 sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the AZFs and STZ bind to A(G/C)T repeats within an EP2 sequence”
e1“Arabidopsis L. Heynh.”
Claim · model-written summary
AZFs and STZ preferentially localize to the nucleus.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- AZFs and STZe0
- Outcome
- preferentially localized to the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the AZFs and STZ are preferentially localized to the nucleus”
e1“Arabidopsis L. Heynh.”
Claim · model-written summary
Four ZPT2-related proteins repress transactivation by other transcription factors.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- ZPT2-related proteinse0
- Outcome
- transactivation activity of other transcription factorse0
- Direction
- down-regulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These four ZPT2-related proteins were shown to act as transcriptional repressors that down-regulate the transactivation activity of other transcription factors”
e1“Arabidopsis L. Heynh.”
Claim · model-written summary
Dehydration induced AZF2 and STZ expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- dehydratione0
- Gene
- AZF2 and STZe0
- Outcome
- expression of AZF2 and STZe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of AZF2 and STZ was strongly induced by dehydration, high-salt and cold stresses, and abscisic acid treatment”
e1“Arabidopsis L. Heynh.”
Claim · model-written summary
High-salt stress induced AZF2 and STZ expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- high-salte0
- Gene
- AZF2 and STZe0
- Outcome
- expression of AZF2 and STZe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of AZF2 and STZ was strongly induced by dehydration, high-salt and cold stresses, and abscisic acid treatment”
e1“Arabidopsis L. Heynh.”
Claim · model-written summary
Cold stress induced AZF2 and STZ expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- cold stressese0
- Gene
- AZF2 and STZe0
- Outcome
- expression of AZF2 and STZe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of AZF2 and STZ was strongly induced by dehydration, high-salt and cold stresses, and abscisic acid treatment”
e1“Arabidopsis L. Heynh.”
Claim · model-written summary
Abscisic acid induced AZF2 and STZ expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- abscisic acid treatmente0
- Gene
- AZF2 and STZe0
- Outcome
- expression of AZF2 and STZe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of AZF2 and STZ was strongly induced by dehydration, high-salt and cold stresses, and abscisic acid treatment”
e1“Arabidopsis L. Heynh.”
Claim · model-written summary
Stress induction of AZF2 and STZ was in leaves rather than roots.
- Taxon
- ArabidopsisArabidopsise2
- Organ / stage
- leavese0
- Gene
- AZF2 or STZe1
- Outcome
- both genes are induced in leavese0
- Direction
- inducede0
- Comparator
- rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both genes are induced in leaves rather than roots of rosette plants by the stresses”
e1“AZF2 or STZ promoters”
e2“Arabidopsis L. Heynh.”
Claim · model-written summary
STZ overexpression retarded Arabidopsis growth.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpressing STZe0
- Gene
- STZe0
- Outcome
- growth retardatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic Arabidopsis overexpressing STZ showed growth retardation and tolerance to drought stress”
Claim · model-written summary
STZ overexpression conferred drought tolerance.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpressing STZe0
- Gene
- STZe0
- Outcome
- tolerance to drought stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic Arabidopsis overexpressing STZ showed growth retardation and tolerance to drought stress”
Claim · model-written summary
Authors propose AZF2 and STZ repress transcription to increase stress tolerance following growth retardation.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- AZF2 and STZe0
- Outcome
- increase stress tolerancee0
- Qualifier
- following growth retardatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AZF2 and STZ function as transcriptional repressors to increase stress tolerance following growth retardation”
e1“Arabidopsis L. Heynh.”
The same questions in other papers
cold · molecular: expression · ArabidopsisPositive and negative results
17 papers in the same direction as this paper · 20 in the opposite direction · 1 reporting no difference from an alternative
- positive
- negative
- no effect
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See all 39 papers →drought · molecular: expression · ArabidopsisPositive and negative results
38 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- negative
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.