Abstract
In plants, the importance of phospholipid signaling in responses to environmental stresses is becoming well documented. The involvement of phospholipids in abscisic acid (ABA) responses is also established. In a previous study, we demonstrated that the stimulation of phospholipase D (PLD) activity and plasma membrane anion currents by ABA were both required for RAB18 expression in Arabidopsis thaliana suspension cells. In this study, we show that the total lipids extracted from ABA-treated cells mimic ABA in activating plasmalemma anion currents and induction of RAB18 expression. Moreover, ABA evokes within 5 min a transient 1.7-fold increase in phosphatidic acid (PA) followed by a sevenfold increase in diacylglycerol pyrophosphate (DGPP) at 20 min. PA activated plasmalemma anion currents but was incapable of triggering RAB18 expression. By contrast, DGPP mimicked ABA on anion currents and was also able to stimulate RAB18 expression. Here we show the role of DGPP as phospholipid second messenger in ABA signaling.
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Claims 9
Claim · model-written summary
Lipids from ABA treated cells mimicked ABA activation of plasma membrane anion currents.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- total lipids extracted from ABA-treated cellse0
- Outcome
- plasmalemma anion currentse0
- Direction
- activatinge0
- Comparator
- ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“total lipids extracted from ABA-treated cells mimic ABA in activating plasmalemma anion currents”
e1“Arabidopsis thaliana suspension cells”
Claim · model-written summary
Lipids from ABA treated cells mimicked ABA induction of RAB18 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- total lipids extracted from ABA-treated cellse0
- Gene
- RAB18e0
- Outcome
- RAB18 expressione0
- Direction
- inductione0
- Comparator
- ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“total lipids extracted from ABA-treated cells mimic ABA in activating plasmalemma anion currents and induction of RAB18 expression.”
e1“Arabidopsis thaliana suspension cells”
Claim · model-written summary
ABA caused a transient 1.7 fold increase in phosphatidic acid within five minutes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- ABAe0
- Outcome
- phosphatidic acid (PA)e0
- Direction
- increasee0
- Value
- 1.7-folde0
- Qualifier
- within 5 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA evokes within 5 min a transient 1.7-fold increase in phosphatidic acid (PA)”
e1“Arabidopsis thaliana suspension cells”
Claim · model-written summary
ABA caused a sevenfold increase in diacylglycerol pyrophosphate at 20 minutes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- ABAe0
- Outcome
- diacylglycerol pyrophosphate (DGPP)e0
- Direction
- increasee0
- Value
- sevenfolde0
- Qualifier
- at 20 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA evokes within 5 min a transient 1.7-fold increase in phosphatidic acid (PA) followed by a sevenfold increase in diacylglycerol pyrophosphate (DGPP) at 20 min.”
e1“Arabidopsis thaliana suspension cells”
Claim · model-written summary
PA activated plasma membrane anion currents.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- PAe0
- Outcome
- plasmalemma anion currentse0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PA activated plasmalemma anion currents but was incapable of triggering RAB18 expression.”
e1“Arabidopsis thaliana suspension cells”
Claim · model-written summary
PA did not trigger RAB18 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- PAe0
- Gene
- RAB18e0
- Outcome
- RAB18 expressione0
- Direction
- incapable of triggeringe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PA activated plasmalemma anion currents but was incapable of triggering RAB18 expression.”
e1“Arabidopsis thaliana suspension cells”
Claim · model-written summary
DGPP mimicked ABA stimulation of anion currents.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- DGPPe0
- Outcome
- anion currentse0
- Direction
- mimickede0
- Comparator
- ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DGPP mimicked ABA on anion currents and was also able to stimulate RAB18 expression.”
e1“Arabidopsis thaliana suspension cells”
Claim · model-written summary
DGPP stimulated RAB18 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- DGPPe0
- Gene
- RAB18e0
- Outcome
- RAB18 expressione0
- Direction
- stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DGPP mimicked ABA on anion currents and was also able to stimulate RAB18 expression.”
e1“Arabidopsis thaliana suspension cells”
Claim · model-written summary
DGPP acts as a phospholipid second messenger in ABA signaling.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Compound
- DGPPe0
- Outcome
- ABA signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“role of DGPP as phospholipid second messenger in ABA signaling.”
e1“Arabidopsis thaliana suspension cells”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.