Abstract
Abscisic acid (ABA) is a ubiquitous phytohormone involved in many developmental processes and stress responses of plants. ABA moves within the plant, and intracellular receptors for ABA have been recently identified; however, no ABA transporter has been described to date. Here, we report the identification of the ATP-binding cassette (ABC) transporter Arabidopsis thaliana Pleiotropic drug resistance transporter PDR12 (AtPDR12)/ABCG40 as a plasma membrane ABA uptake transporter. Uptake of ABA into yeast and BY2 cells expressing AtABCG40 was increased, whereas ABA uptake into protoplasts of atabcg40 plants was decreased compared with control cells. In response to exogenous ABA, the up-regulation of ABA responsive genes was strongly delayed in atabcg40 plants, indicating that ABCG40 is necessary for timely responses to ABA. Stomata of loss-of-function atabcg40 mutants closed more slowly in response to ABA, resulting in reduced drought tolerance. Our results integrate ABA-dependent signaling and transport processes and open another avenue for the engineering of drought-tolerant plants.
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Claims 7
Claim · model-written summary
AtPDR12/ABCG40 was identified as a plasma membrane ABA uptake transporter.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- PDR12 (AtPDR12)/ABCG40e0
- Outcome
- plasma membrane ABA uptake transportere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana Pleiotropic drug resistance transporter PDR12 (AtPDR12)/ABCG40 as a plasma membrane ABA uptake transporter”
Claim · model-written summary
ABA uptake increased in yeast expressing AtABCG40.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- yeast and BY2 cells expressing AtABCG40e0
- Gene
- AtABCG40e0
- Outcome
- Uptake of ABA into yeaste0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Uptake of ABA into yeast and BY2 cells expressing AtABCG40 was increased”
e1“Arabidopsis thaliana Pleiotropic drug resistance transporter PDR12 (AtPDR12)/ABCG40”
Claim · model-written summary
ABA uptake increased in BY2 cells expressing AtABCG40.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- BY2 cells expressing AtABCG40e0
- Gene
- AtABCG40e0
- Outcome
- Uptake of ABA into yeast and BY2 cellse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Uptake of ABA into yeast and BY2 cells expressing AtABCG40 was increased”
e1“Arabidopsis thaliana Pleiotropic drug resistance transporter PDR12 (AtPDR12)/ABCG40”
Claim · model-written summary
ABA uptake decreased in atabcg40 protoplasts compared with control cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- atabcg40e0
- Treatment
- atabcg40 plantse0
- Outcome
- ABA uptake into protoplastse0
- Direction
- decreasede0
- Comparator
- control cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA uptake into protoplasts of atabcg40 plants was decreased compared with control cells”
e1“Arabidopsis thaliana Pleiotropic drug resistance transporter PDR12 (AtPDR12)/ABCG40”
Claim · model-written summary
ABA responsive gene up-regulation was delayed in atabcg40 plants after exogenous ABA.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- atabcg40e0
- Treatment
- exogenous ABAe0
- Outcome
- up-regulation of ABA responsive genese0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In response to exogenous ABA, the up-regulation of ABA responsive genes was strongly delayed in atabcg40 plants”
e1“Arabidopsis thaliana Pleiotropic drug resistance transporter PDR12 (AtPDR12)/ABCG40”
Claim · model-written summary
Stomata of atabcg40 mutants closed more slowly in response to ABA.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- loss-of-function atabcg40 mutantse0
- Treatment
- ABAe0
- Outcome
- Stomata of loss-of-function atabcg40 mutants closede0
- Direction
- more slowlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stomata of loss-of-function atabcg40 mutants closed more slowly in response to ABA”
e1“Arabidopsis thaliana Pleiotropic drug resistance transporter PDR12 (AtPDR12)/ABCG40”
Claim · model-written summary
The slower stomatal closure in atabcg40 mutants resulted in reduced drought tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- loss-of-function atabcg40 mutantse0
- Outcome
- reduced drought tolerancee0
- Qualifier
- closed more slowly in response to ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stomata of loss-of-function atabcg40 mutants closed more slowly in response to ABA, resulting in reduced drought tolerance”
e1“Arabidopsis thaliana Pleiotropic drug resistance transporter PDR12 (AtPDR12)/ABCG40”
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