Abstract
• Although proteins in the basic helix-loop-helix (bHLH) family are universal transcription factors in eukaryotes, the biological roles of most bHLH family members are not well understood in plants. • The Arabidopsis thaliana bHLH122 transcripts were strongly induced by drought, NaCl and osmotic stresses, but not by ABA treatment. Promoter::GUS analysis showed that bHLH122 was highly expressed in vascular tissues and guard cells. Compared with wild-type (WT) plants, transgenic plants overexpressing bHLH122 displayed greater resistance to drought, NaCl and osmotic stresses. In contrast, the bhlh122 loss-of-function mutant was more sensitive to NaCl and osmotic stresses than were WT plants. • Microarray analysis indicated that bHLH122 was important for the expression of a number of abiotic stress-responsive genes. In electrophoretic mobility shift assay and chromatin immunoprecipitation assays, bHLH122 could bind directly to the G-box/E-box cis-elements in the CYP707A3 promoter, and repress its expression. Further, up-regulation of bHLH122 substantially increased cellular ABA levels. • These results suggest that bHLH122 functions as a positive regulator of drought, NaCl and osmotic signaling.
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Claims 8
Claim · model-written summary
bHLH122 transcripts were induced by drought, NaCl, and osmotic stress.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- drought, NaCl and osmotic stressese0
- Gene
- bHLH122e0
- Outcome
- bHLH122 transcriptse0
- Direction
- strongly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana bHLH122 transcripts were strongly induced by drought, NaCl and osmotic stresses”
Claim · model-written summary
ABA treatment did not induce bHLH122 transcripts.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- ABA treatmente0
- Gene
- bHLH122e0
- Outcome
- bHLH122 transcriptse0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bHLH122 transcripts were strongly induced by drought, NaCl and osmotic stresses, but not by ABA treatment”
t0“The Arabidopsis thaliana bHLH122 transcripts”
Claim · model-written summary
bHLH122 was highly expressed in vascular tissues and guard cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- vascular tissues and guard cellse0
- Gene
- bHLH122e0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bHLH122 was highly expressed in vascular tissues and guard cells”
t0“The Arabidopsis thaliana bHLH122 transcripts”
Claim · model-written summary
bHLH122 overexpression increased resistance to drought, NaCl, and osmotic stress relative to wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- overexpressing bHLH122e0
- Gene
- bHLH122e0
- Outcome
- resistance to drought, NaCl and osmotic stressese0
- Direction
- greatere0
- Comparator
- wild-type (WT) plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with wild-type (WT) plants, transgenic plants overexpressing bHLH122 displayed greater resistance to drought, NaCl and osmotic stresses”
t0“The Arabidopsis thaliana bHLH122 transcripts”
Claim · model-written summary
The bhlh122 mutant was more sensitive to NaCl and osmotic stresses than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- bhlh122 loss-of-function mutante0
- Treatment
- loss-of-functione0
- Outcome
- sensitive to NaCl and osmotic stressese0
- Direction
- more sensitivee0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the bhlh122 loss-of-function mutant was more sensitive to NaCl and osmotic stresses than were WT plants”
t0“The Arabidopsis thaliana bHLH122 transcripts”
Claim · model-written summary
bHLH122 affected expression of multiple abiotic stress responsive genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- bHLH122e0
- Outcome
- expression of a number of abiotic stress-responsive genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bHLH122 was important for the expression of a number of abiotic stress-responsive genes”
t0“The Arabidopsis thaliana bHLH122 transcripts”
Claim · model-written summary
bHLH122 bound G-box/E-box elements in the CYP707A3 promoter and repressed CYP707A3 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- bHLH122e0
- Outcome
- repress its expressione0
- Qualifier
- bind directly to the G-box/E-box cis-elements in the CYP707A3 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bHLH122 could bind directly to the G-box/E-box cis-elements in the CYP707A3 promoter, and repress its expression”
t0“The Arabidopsis thaliana bHLH122 transcripts”
Claim · model-written summary
Up-regulation of bHLH122 increased cellular ABA levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- up-regulation of bHLH122e0
- Gene
- bHLH122e0
- Outcome
- cellular ABA levelse0
- Direction
- substantially increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“up-regulation of bHLH122 substantially increased cellular ABA levels”
t0“The Arabidopsis thaliana bHLH122 transcripts”
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