Abstract
The precise roles of the B-box zinc finger family of transcription factors in plant stress are poorly understood. Functional analysis was performed on AtCOL4, an Arabidopsis thaliana L. CONSTANS-like 4 protein that is a putative novel transcription factor, and which contains a predicted transcriptional activation domain. Analyses of an AtCOL4 promoter-β-glucuronidase (GUS) construct revealed substantial GUS activity in whole seedlings. The expression of AtCOL4 was strongly induced by abscisic acid (ABA), salt, and osmotic stress. Mutation in atcol4 resulted in increased sensitivity to ABA and salt stress during seed germination and the cotyledon greening process. In contrast, AtCOL4-overexpressing plants were less sensitive to ABA and salt stress compared to the wild type. Interestingly, in the presence of ABA or salt stress, the transcript levels of other ABA biosynthesis and stress-related genes were enhanced induction in AtCOL4-overexpressing and WT plants, rather than in the atcol4 mutant. Thus, AtCOL4 is involved in ABA and salt stress response through the ABA-dependent signaling pathway. Taken together, these findings provide compelling evidence that AtCOL4 is an important regulator for plant tolerance to abiotic stress.
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Claims 11
Claim · model-written summary
The AtCOL4 protein contains a predicted transcriptional activation domain.
- Taxon
- Arabidopsis thaliana L.
Arabidopsis thalianae0 - Gene
- AtCOL4e1
- Outcome
- predicted transcriptional activation domaine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“AtCOL4, an Arabidopsis thaliana L. CONSTANS-like 4 protein that is a putative novel transcription factor, and which contains a predicted transcriptional activation domain”
Claim · model-written summary
AtCOL4 promoter activity was substantial in whole seedlings.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Organ / stage
- whole seedlingse1
- Gene
- AtCOL4e1
- Outcome
- substantial GUS activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“Analyses of an AtCOL4 promoter-β-glucuronidase (GUS) construct revealed substantial GUS activity in whole seedlings”
Claim · model-written summary
ABA strongly induced AtCOL4 expression.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Treatment
- abscisic acid (ABA)e1
- Gene
- AtCOL4e1
- Outcome
- expression of AtCOL4e1
- Direction
- strongly inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“The expression of AtCOL4 was strongly induced by abscisic acid (ABA), salt, and osmotic stress”
Claim · model-written summary
Salt strongly induced AtCOL4 expression.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Treatment
- salte1
- Gene
- AtCOL4e1
- Outcome
- expression of AtCOL4e1
- Direction
- strongly inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“The expression of AtCOL4 was strongly induced by abscisic acid (ABA), salt, and osmotic stress”
Claim · model-written summary
Osmotic stress strongly induced AtCOL4 expression.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Treatment
- osmotic stresse1
- Gene
- AtCOL4e1
- Outcome
- expression of AtCOL4e1
- Direction
- strongly inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“The expression of AtCOL4 was strongly induced by abscisic acid (ABA), salt, and osmotic stress”
Claim · model-written summary
Atcol4 mutation increased ABA sensitivity during germination and cotyledon greening.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Material
- atcol4e1
- Organ / stage
- seed germination and the cotyledon greening processe1
- Treatment
- ABAe1
- Outcome
- increased sensitivity to ABAe1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“Mutation in atcol4 resulted in increased sensitivity to ABA and salt stress during seed germination and the cotyledon greening process”
Claim · model-written summary
Atcol4 mutation increased salt sensitivity during germination and cotyledon greening.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Material
- atcol4e1
- Organ / stage
- seed germination and the cotyledon greening processe1
- Treatment
- salt stresse1
- Outcome
- increased sensitivity to ABA and salt stresse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“Mutation in atcol4 resulted in increased sensitivity to ABA and salt stress during seed germination and the cotyledon greening process”
Claim · model-written summary
AtCOL4 overexpression reduced ABA sensitivity relative to wild type.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Material
- AtCOL4-overexpressing plantse1
- Treatment
- ABAe1
- Outcome
- less sensitive to ABAe1
- Direction
- lesse1
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“AtCOL4-overexpressing plants were less sensitive to ABA and salt stress compared to the wild type”
Claim · model-written summary
AtCOL4 overexpression reduced salt sensitivity relative to wild type.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Material
- AtCOL4-overexpressing plantse1
- Treatment
- salt stresse1
- Outcome
- less sensitive to ABA and salt stresse1
- Direction
- lesse1
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“AtCOL4-overexpressing plants were less sensitive to ABA and salt stress compared to the wild type”
Claim · model-written summary
ABA biosynthesis and stress gene transcripts were induced more in AtCOL4 overexpressors and wild type than atcol4 mutants under ABA or salt stress.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Material
- AtCOL4-overexpressing and WT plantse1
- Treatment
- ABA or salt stresse1
- Outcome
- transcript levels of other ABA biosynthesis and stress-related genese1
- Direction
- enhanced inductione1
- Comparator
- atcol4 mutante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“in the presence of ABA or salt stress, the transcript levels of other ABA biosynthesis and stress-related genes were enhanced induction in AtCOL4-overexpressing and WT plants, rather than in the atcol4 mutant”
Claim · model-written summary
The authors infer that AtCOL4 mediates ABA and salt responses via ABA-dependent signaling.
- Taxon
- Arabidopsis thaliana L.Arabidopsis thalianae0
- Gene
- AtCOL4e1
- Outcome
- ABA and salt stress responsee1
- Qualifier
- ABA-dependent signaling pathwaye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana L.”
e1“AtCOL4 is involved in ABA and salt stress response through the ABA-dependent signaling pathway”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.