Abstract
Plant-pathogenic fungi employ a variety of infection strategies; as a result, fungi probably rely on different sets of proteins for successful infection. The F-box protein Frp1, only present in filamentous fungi belonging to the Sordariomycetes, Leotiomycetes and Dothideomycetes, is required for nonsugar carbon catabolism and pathogenicity in the root-infecting fungus Fusarium oxysporum. To assess the role of Frp1 in other plant-pathogenic fungi, FRP1 deletion mutants were generated in Fusarium graminearum and Botrytis cinerea, and their phenotypes were analysed. Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts. Deletion of BcFRP1 in B. cinerea did not show any effect on pathogenicity. Sexual reproduction, however, was impaired in both F. graminearum and B. cinerea FRP1 deletion mutants. The mutants of all three fungi displayed different phenotypes when grown on an array of carbon sources. The F. oxysporum and B. cinerea deletion mutants showed opposite growth phenotypes on sugar and nonsugar carbon sources. Replacement of FoFRP1 in F. oxysporum with the B. cinerea BcFRP1 resulted in the restoration of pathogenicity, but also in a switch from impaired growth on nonsugar carbon sources to impaired growth on sugar carbon sources. This effect could be ascribed in part to the B. cinerea BcFRP1 promoter sequence. In conclusion, the function of the F-box protein Frp1, despite its high sequence conservation, is not conserved between different fungi, leading to differential requirements for pathogenicity and carbon source utilization.
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Claims 11
Claim · model-written summary
Deleting FgFRP1 in F. graminearum impaired infection of barley roots.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree1 - Organ / stage
- rootse1
- Treatment
- Deletion of FgFRP1 in F. graminearume1
- Interactor
- F. graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume1pathogen - Gene
- FgFRP1e1
- Outcome
- infection of barley rootse1
- Direction
- impairede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
Claim · model-written summary
Deleting FgFRP1 in F. graminearum did not reduce infection of aerial plant parts.
- Taxon
- barleyHordeum vulgaree1
- Organ / stage
- aerial plant partse1
- Treatment
- Deletion of FgFRP1e1
- Interactor
- F. graminearumFusarium graminearume1pathogen
- Outcome
- infectione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
Claim · model-written summary
Deleting BcFRP1 in B. cinerea had no detectable effect on pathogenicity.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Deletion of BcFRP1 in B. cinereae2
- Interactor
- B. cinerea
Botrytis cinereae2pathogen - Gene
- BcFRP1e2
- Outcome
- pathogenicitye2
- Direction
- no_changeinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
e2“Deletion of BcFRP1 in B. cinerea did not show any effect on pathogenicity”
Claim · model-written summary
FRP1 deletion impaired sexual reproduction in F. graminearum.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- FRP1 deletion mutantse3
- Interactor
- F. graminearumFusarium graminearume3pathogen
- Outcome
- Sexual reproductione3
- Direction
- impairede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
e3“Sexual reproduction, however, was impaired in both F. graminearum and B. cinerea FRP1 deletion mutants”
Claim · model-written summary
FRP1 deletion impaired sexual reproduction in B. cinerea.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- FRP1 deletion mutantse3
- Interactor
- B. cinereaBotrytis cinereae3pathogen
- Outcome
- Sexual reproductione3
- Direction
- impairede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
e3“Sexual reproduction, however, was impaired in both F. graminearum and B. cinerea FRP1 deletion mutants”
Claim · model-written summary
The F. oxysporum and B. cinerea deletion mutants showed opposite growth phenotypes on sugar and nonsugar carbon sources.
- Taxon
- barleyHordeum vulgaree1
- Interactor
- F. oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume5pathogen - Second interactor
- B. cinereaBotrytis cinereae5pathogen
- Outcome
- growth phenotypes on sugar and nonsugar carbon sourcese5
- Qualifier
- oppositee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
e5“The F. oxysporum and B. cinerea deletion mutants showed opposite growth phenotypes on sugar and nonsugar carbon sources”
Claim · model-written summary
Deletion mutants of all three fungi showed different phenotypes when grown on an array of carbon sources.
- Taxon
- barleyHordeum vulgaree1
- Outcome
- different phenotypes when grown on an array of carbon sourcese4
- Qualifier
- all three fungie4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
e4“The mutants of all three fungi displayed different phenotypes when grown on an array of carbon sources”
Claim · model-written summary
Replacing FoFRP1 in F. oxysporum with BcFRP1 from B. cinerea restored pathogenicity.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Replacement of FoFRP1 in F. oxysporum with the B. cinerea BcFRP1e6
- Interactor
- F. oxysporumFusarium oxysporume6pathogen
- Gene
- BcFRP1e6
- Outcome
- pathogenicitye6
- Direction
- restoratione6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
e6“Replacement of FoFRP1 in F. oxysporum with the B. cinerea BcFRP1 resulted in the restoration of pathogenicity, but also in a switch from impaired growth on nonsugar carbon sources to impaired growth on sugar carbon sources”
Claim · model-written summary
The same replacement shifted F. oxysporum growth from impaired on nonsugar carbon sources to impaired on sugar carbon sources.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Replacement of FoFRP1 in F. oxysporum with the B. cinerea BcFRP1e6
- Interactor
- F. oxysporumFusarium oxysporume6pathogen
- Outcome
- growth on sugar carbon sourcese6
- Direction
- switche6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
e6“Replacement of FoFRP1 in F. oxysporum with the B. cinerea BcFRP1 resulted in the restoration of pathogenicity, but also in a switch from impaired growth on nonsugar carbon sources to impaired growth on sugar carbon sources”
Claim · model-written summary
The changed carbon-source growth phenotype may be partly due to the BcFRP1 promoter sequence.
- Taxon
- barleyHordeum vulgaree1
- Gene
- BcFRP1 promoter sequencee7
- Outcome
- This effecte7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
e7“This effect could be ascribed in part to the B. cinerea BcFRP1 promoter sequence”
Claim · model-written summary
The function of Frp1 is not conserved between different fungi.
- Taxon
- barleyHordeum vulgaree1
- Gene
- Frp1e7
- Outcome
- function of the F-box protein Frp1e7
- Qualifier
- not conserved between different fungie7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgFRP1 in F. graminearum led to impaired infection of barley roots, but not of aerial plant parts”
e7“the function of the F-box protein Frp1, despite its high sequence conservation, is not conserved between different fungi, leading to differential requirements for pathogenicity and carbon source utilization”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.