Abstract
Whole-transcriptome analysis was used here for the first time in the rhizosphere to discern the genes involved in the pathogenic response of Pseudomonas aeruginosa PAO1 as well as to discern the response of the poplar tree. Differential gene expression shows that 185 genes of the bacterium and 753 genes of the poplar tree were induced in the rhizosphere. Using the P. aeruginosa transcriptome analysis, isogenic knockout mutants, and two novel plant assays (poplar and barley), seven novel PAO1 virulence genes were identified (PA1385, PA2146, PA2462, PA2463, PA2663, PA4150 and PA4295). The uncharacterized putative haemolysin repressor, PA2463, upon inactivation, resulted in greater poplar virulence and elevated haemolysis while this mutant remained competitive in the rhizosphere. In addition, disruption of the haemolysin gene itself (PA2462) reduced the haemolytic activity of P. aeruginosa, caused less cytotoxicity and reduced barley virulence, as expected. Inactivating PA1385, a putative glycosyl transferase, reduced both poplar and barley virulence. Furthermore, disrupting PA2663, a putative membrane protein, reduced biofilm formation by 20-fold. Inactivation of PA3476 (rhlI) increased virulence with barley as well as haemolytic activity and cytotoxicity, so quorum sensing is important in plant pathogenesis. Hence, this strategy is capable of elucidating virulence genes for an important pathogen.
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Claims 15
Claim · model-written summary
In the rhizosphere, 753 genes of the poplar tree were induced.
- Interactor
- Pseudomonas aeruginosa PAO1
Pseudomonas aeruginosa PAO1t0pathogen - Outcome
- genes of the poplar treee0
- Value
- 753 genese0
- Setting
- rhizospheree0
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pseudomonas aeruginosa PAO1 virulence factors and poplar tree response in the rhizosphere”
e0“Differential gene expression shows that 185 genes of the bacterium and 753 genes of the poplar tree were induced in the rhizosphere”
Claim · model-written summary
In the rhizosphere, 185 genes of the bacterium were induced.
- Interactor
- Pseudomonas aeruginosa PAO1Pseudomonas aeruginosa PAO1t0pathogen
- Outcome
- genes of the bacteriume0
- Value
- 185 genese0
- Setting
- rhizospheree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pseudomonas aeruginosa PAO1 virulence factors and poplar tree response in the rhizosphere”
e0“Differential gene expression shows that 185 genes of the bacterium and 753 genes of the poplar tree were induced in the rhizosphere”
Claim · model-written summary
Seven novel P. aeruginosa PAO1 virulence genes were identified using the transcriptome, knockout mutants and poplar and barley assays.
- Interactor
- P. aeruginosaPseudomonas aeruginosae1pathogen
- Gene
- PA1385, PA2146, PA2462, PA2463, PA2663, PA4150 and PA4295e1
- Outcome
- seven novel PAO1 virulence genese1
- Value
- sevene1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Using the P. aeruginosa transcriptome analysis, isogenic knockout mutants, and two novel plant assays (poplar and barley), seven novel PAO1 virulence genes were identified (PA1385, PA2146, PA2462, PA2463, PA2663, PA4150 and PA4295)”
Claim · model-written summary
Inactivation of PA2463 resulted in greater virulence on poplar.
- Treatment
- upon inactivatione2
- Interactor
- Pseudomonas aeruginosa PAO1Pseudomonas aeruginosa PAO1t0pathogen
- Gene
- PA2463e2
- Outcome
- poplar virulencee2
- Direction
- greatere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The uncharacterized putative haemolysin repressor, PA2463, upon inactivation, resulted in greater poplar virulence and elevated haemolysis while this mutant remained competitive in the rhizosphere”
t0“Pseudomonas aeruginosa PAO1 virulence factors and poplar tree response in the rhizosphere”
Claim · model-written summary
Inactivation of PA2463 elevated haemolysis.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The uncharacterized putative haemolysin repressor, PA2463, upon inactivation, resulted in greater poplar virulence and elevated haemolysis while this mutant remained competitive in the rhizosphere”
Claim · model-written summary
The PA2463 inactivation mutant remained competitive in the rhizosphere.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The uncharacterized putative haemolysin repressor, PA2463, upon inactivation, resulted in greater poplar virulence and elevated haemolysis while this mutant remained competitive in the rhizosphere”
Claim · model-written summary
Disruption of the haemolysin gene PA2462 reduced the haemolytic activity of P. aeruginosa.
- Treatment
- disruption of the haemolysin gene itselfe3
- Interactor
- P. aeruginosaPseudomonas aeruginosae3pathogen
- Gene
- PA2462e3
- Outcome
- haemolytic activitye3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“In addition, disruption of the haemolysin gene itself (PA2462) reduced the haemolytic activity of P. aeruginosa, caused less cytotoxicity and reduced barley virulence, as expected”
t0“Pseudomonas aeruginosa PAO1 virulence factors and poplar tree response in the rhizosphere”
Claim · model-written summary
Disruption of the haemolysin gene PA2462 caused less cytotoxicity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“In addition, disruption of the haemolysin gene itself (PA2462) reduced the haemolytic activity of P. aeruginosa, caused less cytotoxicity and reduced barley virulence, as expected”
t0“Pseudomonas aeruginosa PAO1 virulence factors and poplar tree response in the rhizosphere”
Claim · model-written summary
Disruption of the haemolysin gene PA2462 reduced virulence on barley.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree3 - Treatment
- disruption of the haemolysin gene itselfe3
- Gene
- PA2462e3
- Outcome
- barley virulencee3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e3“In addition, disruption of the haemolysin gene itself (PA2462) reduced the haemolytic activity of P. aeruginosa, caused less cytotoxicity and reduced barley virulence, as expected”
Claim · model-written summary
Inactivation of PA1385 reduced virulence on poplar.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Inactivating PA1385, a putative glycosyl transferase, reduced both poplar and barley virulence”
Claim · model-written summary
Inactivation of PA1385 reduced virulence on barley.
- Taxon
- barleyHordeum vulgaree4
- Treatment
- Inactivating PA1385e4
- Gene
- PA1385e4
- Outcome
- virulencee4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Inactivating PA1385, a putative glycosyl transferase, reduced both poplar and barley virulence”
Claim · model-written summary
Disruption of PA2663 reduced biofilm formation by 20-fold.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pseudomonas aeruginosa PAO1 virulence factors and poplar tree response in the rhizosphere”
e5“Furthermore, disrupting PA2663, a putative membrane protein, reduced biofilm formation by 20-fold”
Claim · model-written summary
Inactivation of PA3476 (rhlI) increased virulence on barley.
- Taxon
- barleyHordeum vulgaree6
- Treatment
- Inactivation of PA3476 (rhlI)e6
- Gene
- rhlIe6
- Outcome
- virulencee6
- Direction
- increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Inactivation of PA3476 (rhlI) increased virulence with barley as well as haemolytic activity and cytotoxicity, so quorum sensing is important in plant pathogenesis”
Claim · model-written summary
Inactivation of PA3476 (rhlI) increased haemolytic activity and cytotoxicity.
- Taxon
- barleyHordeum vulgaree6
- Treatment
- Inactivation of PA3476 (rhlI)e6
- Gene
- rhlIe6
- Outcome
- haemolytic activity and cytotoxicitye6
- Direction
- increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Inactivation of PA3476 (rhlI) increased virulence with barley as well as haemolytic activity and cytotoxicity, so quorum sensing is important in plant pathogenesis”
Claim · model-written summary
The authors conclude that quorum sensing is important in plant pathogenesis.
- Taxon
- barleyHordeum vulgaree6
- Outcome
- plant pathogenesise6
- Qualifier
- quorum sensinge6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Inactivation of PA3476 (rhlI) increased virulence with barley as well as haemolytic activity and cytotoxicity, so quorum sensing is important in plant pathogenesis”
The same questions in other papers
loss of function · disease · Hordeum vulgare (barley)Positive and negative results
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See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.