Abstract
ATP-binding cassette (ABC) transporters mediate active efflux of natural and synthetic toxicants and are considered to be important for drug tolerance in microorganisms. In biological control agents (BCA), ABC transporters can play important roles in antagonism by providing protection against toxins derived from the fungal prey and by mediating the secretion of endogenous toxins. In the present study, we generated deletion and complementation strains of the ABC transporter abcG5 in the fungal BCA Clonostachys rosea to study its role in xenobiotic tolerance and antagonism. Gene expression analysis shows induced expression of abcG5 in the presence of the Fusarium mycotoxin zearalenone (ZEA), secreted metabolites of F. graminearum, and different classes of fungicides. Phenotypic analysis of abcG5 deletion and complementation strains showed that the deletion strains were more sensitive towards F. graminearum culture filtrates, ZEA, and iprodione- and mefenoxam-based fungicides, thus suggesting the involvement of abcG5 in cell protection. The ΔabcG5 strains displayed reduced antagonism towards F. graminearum in a plate confrontation assay. Furthermore, the ΔabcG5 strains failed to protect barley seedlings from F. graminearium foot rot disease. These data show that the abcG5 ABC transporter is important for xenobiotic tolerance and biocontrol traits in C. rosea.
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Claims 8
Claim · model-written summary
abcG5 expression was induced by zearalenone.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree1 - Treatment
- Fusarium mycotoxin zearalenone (ZEA)e0
- Gene
- abcG5e0
- Outcome
- induced expression of abcG5e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Gene expression analysis shows induced expression of abcG5 in the presence of the Fusarium mycotoxin zearalenone (ZEA)”
e1“barley seedlings”
Claim · model-written summary
abcG5 expression was induced by F. graminearum metabolites.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- secreted metabolites of F. graminearume0
- Interactor
- F. graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Gene
- abcG5e0
- Outcome
- induced expression of abcG5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“induced expression of abcG5 in the presence of the Fusarium mycotoxin zearalenone (ZEA), secreted metabolites of F. graminearum”
e1“barley seedlings”
Claim · model-written summary
abcG5 expression was induced by different fungicide classes.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- different classes of fungicidese0
- Gene
- abcG5e0
- Outcome
- induced expression of abcG5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induced expression of abcG5 in the presence of the Fusarium mycotoxin zearalenone (ZEA), secreted metabolites of F. graminearum, and different classes of fungicides”
e1“barley seedlings”
Claim · model-written summary
abcG5 deletion strains were more sensitive to F. graminearum culture filtrates.
- Taxon
- barleyHordeum vulgaree1
- Material
- deletion strainse0
- Treatment
- F. graminearum culture filtratese0
- Interactor
- F. graminearumFusarium graminearume0pathogen
- Outcome
- sensitive towards F. graminearum culture filtratese0
- Direction
- more sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the deletion strains were more sensitive towards F. graminearum culture filtrates, ZEA, and iprodione- and mefenoxam-based fungicides”
e1“barley seedlings”
Claim · model-written summary
abcG5 deletion strains were more sensitive to ZEA.
- Taxon
- barleyHordeum vulgaree1
- Material
- deletion strainse0
- Treatment
- ZEAe0
- Outcome
- sensitive towards F. graminearum culture filtrates, ZEAe0
- Direction
- more sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the deletion strains were more sensitive towards F. graminearum culture filtrates, ZEA, and iprodione- and mefenoxam-based fungicides”
e1“barley seedlings”
Claim · model-written summary
abcG5 deletion strains were more sensitive to iprodione-based fungicides.
- Taxon
- barleyHordeum vulgaree1
- Material
- deletion strainse0
- Treatment
- iprodione-e0
- Outcome
- sensitive towards F. graminearum culture filtrates, ZEA, and iprodione- and mefenoxam-based fungicidese0
- Direction
- more sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the deletion strains were more sensitive towards F. graminearum culture filtrates, ZEA, and iprodione- and mefenoxam-based fungicides”
e1“barley seedlings”
Claim · model-written summary
abcG5 deletion strains showed reduced antagonism toward F. graminearum in a plate assay.
- Taxon
- barleyHordeum vulgaree1
- Material
- ΔabcG5 strainse0
- Treatment
- ΔabcG5 strainse0
- Interactor
- F. graminearumFusarium graminearume0pathogen
- Outcome
- antagonism towards F. graminearume0
- Direction
- reducede0
- Setting
- plate confrontation assaye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ΔabcG5 strains displayed reduced antagonism towards F. graminearum in a plate confrontation assay”
e1“barley seedlings”
Claim · model-written summary
ΔabcG5 strains failed to protect barley seedlings from F. graminearium foot rot.
- Taxon
- barleyHordeum vulgaree0
- Material
- ΔabcG5 strainse0
- Treatment
- ΔabcG5 strainse0
- Interactor
- F. gramineariume0pathogen
- Outcome
- protect barley seedlings from F. graminearium foot rot diseasee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ΔabcG5 strains failed to protect barley seedlings from F. graminearium foot rot disease”
The same questions in other papers
Fusarium graminearum · organism · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.