Abstract
Iron-deficiency responses comprise molecular, physiological and developmental adjustments, ultimately leading to an improved cellular Fe homeostasis. By using a proteomic approach, we identified the ubiquitin-conjugating enzyme UBC13 as being highly responsive to the Fe regime at the post-transcriptional level in the tips of cucumber (Cucumis sativus) roots. UBC13 has been shown to catalyze non-canonical Lys63-linked ubiquitin chains, playing important roles in signal transduction among eukaryotes. Ectopic expression of the cucumber UBC13 gene in Arabidopsis thaliana led to a more pronounced and Fe-responsive formation of branched root hairs, a key response of Arabidopsis roots to Fe deficiency. Plants carrying a mutation in the Arabidopsis ortholog UBC13A were unable to form branched root hairs upon Fe deficiency and showed a perturbed expression of Fe-regulated genes. Mutants defective in both Arabidopsis UBC13 genes, UBC13A and UBC13B, showed a marked reduction in root hair density. Mutations in the cognate E3 ligases RGLG2 and RGLG1 caused the constitutive formation of branched root hairs independent of the Fe supply, indicating the involvement of polyubiquitination in the altered differentiation of rhizodermal cells. It is concluded that UBC13, probably via the formation of Lys63-linked ubiquitin chains, has a critical function in epidermal cell differentiation and is crucial for the regulation of Fe-responsive genes and developmental responses to Fe deficiency.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Cucumber root-tip UBC13 protein responded strongly to iron regime at the post-transcriptional level.
- Taxon
- cucumber (Cucumis sativus)
Cucumis sativuse0 - Organ / stage
- tips of cucumber (Cucumis sativus) rootse0
- Gene
- UBC13e0
- Outcome
- highly responsive to the Fe regime at the post-transcriptional levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ubiquitin-conjugating enzyme UBC13 as being highly responsive to the Fe regime at the post-transcriptional level in the tips of cucumber (Cucumis sativus) roots”
Claim · model-written summary
Ectopic cucumber UBC13 expression enhanced iron-responsive branched root hair formation in Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Ectopic expression of the cucumber UBC13 genee0
- Gene
- cucumber UBC13e0
- Outcome
- formation of branched root hairse0
- Direction
- more pronouncede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of the cucumber UBC13 gene in Arabidopsis thaliana led to a more pronounced and Fe-responsive formation of branched root hairs”
Claim · model-written summary
Arabidopsis UBC13A mutants could not form branched root hairs under iron deficiency.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- mutation in the Arabidopsis ortholog UBC13Ae0
- Gene
- UBC13Ae0
- Outcome
- unable to form branched root hairse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants carrying a mutation in the Arabidopsis ortholog UBC13A were unable to form branched root hairs upon Fe deficiency”
Claim · model-written summary
Arabidopsis UBC13A mutants had perturbed expression of iron-regulated genes.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- mutation in the Arabidopsis ortholog UBC13Ae0
- Gene
- UBC13Ae0
- Outcome
- perturbed expression of Fe-regulated genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation in the Arabidopsis ortholog UBC13A were unable to form branched root hairs upon Fe deficiency and showed a perturbed expression of Fe-regulated genes”
Claim · model-written summary
Double UBC13A/UBC13B mutants had markedly reduced root hair density.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Mutants defective in both Arabidopsis UBC13 genes, UBC13A and UBC13Be0
- Gene
- UBC13A and UBC13Be0
- Outcome
- root hair densitye0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutants defective in both Arabidopsis UBC13 genes, UBC13A and UBC13B, showed a marked reduction in root hair density”
Claim · model-written summary
RGLG2 and RGLG1 mutations caused constitutive branched root hairs regardless of iron supply.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Mutations in the cognate E3 ligases RGLG2 and RGLG1e0
- Gene
- RGLG2 and RGLG1e0
- Outcome
- constitutive formation of branched root hairse0
- Qualifier
- independent of the Fe supplye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in the cognate E3 ligases RGLG2 and RGLG1 caused the constitutive formation of branched root hairs independent of the Fe supply”
t0“Arabidopsis”
Claim · model-written summary
The authors infer UBC13 regulates epidermal differentiation and iron-responsive genes, probably through Lys63-linked ubiquitin chains.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- UBC13e0
- Outcome
- epidermal cell differentiatione0
- Qualifier
- probably via the formation of Lys63-linked ubiquitin chainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UBC13, probably via the formation of Lys63-linked ubiquitin chains, has a critical function in epidermal cell differentiation and is crucial for the regulation of Fe-responsive genes and developmental responses to Fe deficiency”
t0“Arabidopsis”
The same questions in other papers
overexpression · growth · Arabidopsis thaliana (thale cress)Positive and negative results
14 papers in the same direction as this paper · 15 in the opposite direction
- negative
- negative
- negative
26 papers more in this group
See all 30 papers →loss of function · growth · ArabidopsisPositive and negative results
182 papers in the same direction as this paper · 51 in the opposite direction
- negative
- positive
- positive
230 papers more in this group
See all 234 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.