Abstract
The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation and has homology to the PHD-finger class of transcription factors; however, its role in pollen development has not been fully defined. We show that MS1 transcription appears to be autoregulated by the wild-type MS1 transcript or protein. Using a functional green fluorescent protein (GFP) fusion to analyze the temporal and spatial expression of MS1, we demonstrate that the MS1:GFP protein is nuclear localized within the tapetum and is expressed in a developmentally regulated manner between late tetraspore and microspore release, then rapidly breaks down, probably by ubiquitin-dependent proteolysis. Absence of MS1 expression results in changes in tapetal secretion and exine structure. Microarray analysis has shown that 260 (228 downregulated and 32 upreglated) genes have altered expression in young ms1 buds. These genes are primarily associated with pollen wall and coat formation; however, a number of transcription factors and Cys proteases have also been identified as the putative primary regulatory targets of MS1. Ectopic expression of MS1 alters transcriptional regulation of vegetative gene expression, resulting in stunted plants with increased levels of branching, partially fertile flowers and an apparent increase in wall material on mature pollen. MS1 therefore plays a critical role in the induction of pollen wall and pollen coat materials in the tapetum and, ultimately, the production of viable pollen.
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Claims 16
Claim · model-written summary
MS1 transcription appears to be autoregulated by MS1 transcript or protein in Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- MS1e0
- Outcome
- MS1 transcriptione1
- Qualifier
- autoregulated by the wild-type MS1 transcript or proteine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e1“MS1 transcription appears to be autoregulated by the wild-type MS1 transcript or protein”
Claim · model-written summary
The MS1:GFP protein is nuclear localized within the tapetum.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- tapetume2
- Gene
- MS1:GFP proteine2
- Outcome
- nuclear localized within the tapetume2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e2“the MS1:GFP protein is nuclear localized within the tapetum and is expressed in a developmentally regulated manner between late tetraspore and microspore release, then rapidly breaks down, probably by ubiquitin-dependent proteolysis”
Claim · model-written summary
MS1:GFP is expressed in a developmentally regulated manner between late tetraspore and microspore release.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- between late tetraspore and microspore releasee2
- Outcome
- expressed in a developmentally regulated mannere2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e2“the MS1:GFP protein is nuclear localized within the tapetum and is expressed in a developmentally regulated manner between late tetraspore and microspore release, then rapidly breaks down, probably by ubiquitin-dependent proteolysis”
Claim · model-written summary
The MS1:GFP protein rapidly breaks down, probably by ubiquitin-dependent proteolysis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- MS1:GFP proteine2
- Outcome
- rapidly breaks downe2
- Qualifier
- probably by ubiquitin-dependent proteolysise2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e2“the MS1:GFP protein is nuclear localized within the tapetum and is expressed in a developmentally regulated manner between late tetraspore and microspore release, then rapidly breaks down, probably by ubiquitin-dependent proteolysis”
Claim · model-written summary
Absence of MS1 expression changes tapetal secretion and exine structure in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Absence of MS1 expressione3
- Outcome
- changes in tapetal secretion and exine structuree3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e3“Absence of MS1 expression results in changes in tapetal secretion and exine structure”
Claim · model-written summary
Microarray analysis shows that 260 genes have altered expression in young ms1 buds.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- ms1e4
- Organ / stage
- young ms1 budse4
- Outcome
- genes have altered expressione4
- Value
- 260e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e4“Microarray analysis has shown that 260 (228 downregulated and 32 upreglated) genes have altered expression in young ms1 buds”
Claim · model-written summary
Of the altered genes in ms1 buds, 228 are downregulated.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- ms1e4
- Outcome
- downregulatede4
- Value
- 228e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e4“Microarray analysis has shown that 260 (228 downregulated and 32 upreglated) genes have altered expression in young ms1 buds”
Claim · model-written summary
Of the altered genes in ms1 buds, 32 are upregulated.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- upreglatede4
- Value
- 32e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e4“Microarray analysis has shown that 260 (228 downregulated and 32 upreglated) genes have altered expression in young ms1 buds”
Claim · model-written summary
The genes altered in ms1 buds are primarily associated with pollen wall and coat formation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- associated with pollen wall and coat formatione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e5“These genes are primarily associated with pollen wall and coat formation”
Claim · model-written summary
Transcription factors and Cys proteases are putative primary regulatory targets of MS1 in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- transcription factors and Cys proteasese6
- Outcome
- putative primary regulatory targets of MS1e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e6“a number of transcription factors and Cys proteases have also been identified as the putative primary regulatory targets of MS1”
Claim · model-written summary
Ectopic expression of MS1 alters transcriptional regulation of vegetative gene expression in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Ectopic expression of MS1e7
- Outcome
- transcriptional regulation of vegetative gene expressione7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e7“Ectopic expression of MS1 alters transcriptional regulation of vegetative gene expression, resulting in stunted plants with increased levels of branching, partially fertile flowers and an apparent increase in wall material on mature pollen”
Claim · model-written summary
Ectopic expression of MS1 increased the levels of branching in Arabidopsis thaliana.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Ectopic expression of MS1e7
- Outcome
- levels of branchinge7
- Direction
- increasede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e7“Ectopic expression of MS1 alters transcriptional regulation of vegetative gene expression, resulting in stunted plants with increased levels of branching, partially fertile flowers and an apparent increase in wall material on mature pollen”
Claim · model-written summary
Ectopic expression of MS1 results in stunted plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Ectopic expression of MS1e7
- Outcome
- stunted plantse7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e7“Ectopic expression of MS1 alters transcriptional regulation of vegetative gene expression, resulting in stunted plants with increased levels of branching, partially fertile flowers and an apparent increase in wall material on mature pollen”
Claim · model-written summary
Ectopic expression of MS1 results in partially fertile flowers.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Ectopic expression of MS1e7
- Outcome
- partially fertile flowerse7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e7“Ectopic expression of MS1 alters transcriptional regulation of vegetative gene expression, resulting in stunted plants with increased levels of branching, partially fertile flowers and an apparent increase in wall material on mature pollen”
Claim · model-written summary
Ectopic expression of MS1 apparently increases wall material on mature pollen.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Ectopic expression of MS1e7
- Outcome
- wall material on mature pollene7
- Direction
- increasee7
- Qualifier
- apparente7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e7“Ectopic expression of MS1 alters transcriptional regulation of vegetative gene expression, resulting in stunted plants with increased levels of branching, partially fertile flowers and an apparent increase in wall material on mature pollen”
Claim · model-written summary
MS1 plays a critical role in inducing pollen wall and pollen coat materials in the tapetum and in producing viable pollen.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- MS1e1
- Outcome
- the production of viable pollene1
- Qualifier
- the induction of pollen wall and pollen coat materials in the tapetume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation”
e1“MS1 therefore plays a critical role in the induction of pollen wall and pollen coat materials in the tapetum and, ultimately, the production of viable pollen”
The same questions in other papers
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See all 30 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.