Abstract
Iron is an essential micronutrient that plays important roles as a redox cofactor in a variety of processes, many of which are related to DNA metabolism. The E2 ubiquitin conjugase UBC13, the only E2 protein that is capable of catalyzing the formation of non-canonical K63-linked ubiquitin chains, has been associated with the DNA damage tolerance pathway in eukaryotes, critical for maintenance of genome stability and integrity. We previously showed that UBC13 and an interacting E3 ubiquitin ligase, RGLG, affect the differentiation of root epidermal cells in Arabidopsis. When grown on iron-free media, Arabidopsis plants develops root hairs that are branched at their base, a response that has been interpreted as an adaption to reduced iron availability. Mutations in UBC13A abolished the branched root hair phenotype. Unexpectedly, mutations in RGLG genes caused constitutive root hair branching. Based on recent results that link endocytotic turnover of plasma membrane-bound PIN transporters to K63-linked ubiquitination, we reinterpreted our results in a context that classifies the root hair phenotype of iron-deficient plants as a consequence of altered auxin distribution. We show here that UBC13A/B and RGLG1/2 are involved in DNA damage repair and hypothesize that UBC13 protein becomes limited under iron-deficient conditions to prioritize DNA metabolism. The data suggest that genes involved in combating detrimental effects on genome stability may represent essential components in the plant's stress response.
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Claims 6
Claim · model-written summary
Iron-free media induced branched root hairs.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- iron-free mediae0
- Outcome
- root hairs that are branched at their basee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When grown on iron-free media, Arabidopsis plants develops root hairs that are branched at their base”
Claim · model-written summary
UBC13A mutations abolished branched root hairs.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Mutations in UBC13Ae0
- Gene
- UBC13Ae0
- Outcome
- branched root hair phenotypee0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in UBC13A abolished the branched root hair phenotype”
t0“Arabidopsis”
Claim · model-written summary
RGLG mutations caused constitutive root hair branching.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- mutations in RGLG genese0
- Gene
- RGLGe0
- Outcome
- root hair branchinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations in RGLG genes caused constitutive root hair branching”
t0“Arabidopsis”
Claim · model-written summary
UBC13A/B and RGLG1/2 participate in DNA damage repair.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- UBC13A/B and RGLG1/2e0
- Outcome
- DNA damage repaire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UBC13A/B and RGLG1/2 are involved in DNA damage repair”
t0“Arabidopsis”
Claim · model-written summary
The authors hypothesize that iron deficiency limits UBC13 protein to prioritize DNA metabolism.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- UBC13e0
- Outcome
- DNA metabolisme0
- Qualifier
- iron-deficient conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hypothesize that UBC13 protein becomes limited under iron-deficient conditions to prioritize DNA metabolism”
t0“Arabidopsis”
Claim · model-written summary
The authors interpret iron-deficient root hair branching as a consequence of altered auxin distribution.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- root hair phenotypee0
- Qualifier
- altered auxin distributione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root hair phenotype of iron-deficient plants as a consequence of altered auxin distribution”
t0“Arabidopsis”
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loss of function · growth · ArabidopsisPositive and negative results
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