Abstract
Specific recognition of Hyaloperonospora parasitica isolate Cala2 by Arabidopsis thaliana Ws-0 is mediated by the resistance gene RPP1A. Transient expression of different truncations of RPP1A in tobacco leaves revealed that its TIR-NB-ARC portion is sufficient to induce an elicitor-independent cell death. In stable transgenic lines of Arabidopsis, overexpression of the RPP1A TIR-NB-ARC domains (E12) using the 35S promoter leads to broad-spectrum resistance to virulent strains of H. parasitica and Pseudomonas syringae DC3000. The TIR-NB-ARC-mediated constitutive immunity is due to activation of the salicylic acid-dependent resistance pathway and is relieved by either a mutation in EDS1 or the presence of the salicylate hydroxylase gene, NahG. Growth of 35S::E12 plants is reduced, a phenotype observed in many constitutively resistant mutants. RPP1A carries a hydrophobic peptide at its N-terminus that directs the RPP1A protein into membranes, though it may not be the sole determinant mediating membrane association of RPP1A. Two-phase partitioning and sucrose density gradient sedimentation established that RPP1A resides in the endoplasmic reticulum and/or Golgi apparatus.
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Claims 9
Claim · model-written summary
The RPP1A TIR-NB-ARC portion induced elicitor-independent cell death in tobacco leaves
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- leavese0
- Treatment
- Transient expression of different truncations of RPP1Ae0
- Gene
- RPP1Ae0
- Outcome
- elicitor-independent cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of different truncations of RPP1A in tobacco leaves revealed that its TIR-NB-ARC portion is sufficient to induce an elicitor-independent cell death”
Claim · model-written summary
E12 overexpression conferred broad-spectrum resistance to H. parasitica
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- overexpression of the RPP1A TIR-NB-ARC domains (E12)e0
- Interactor
- H. parasitica
Hyaloperonospora parasiticae0pathogen - Gene
- RPP1Ae0
- Outcome
- broad-spectrum resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of the RPP1A TIR-NB-ARC domains (E12) using the 35S promoter leads to broad-spectrum resistance to virulent strains of H. parasitica and Pseudomonas syringae DC3000”
e1“stable transgenic lines of Arabidopsis”
Claim · model-written summary
E12 overexpression conferred broad-spectrum resistance to Pseudomonas syringae DC3000
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- overexpression of the RPP1A TIR-NB-ARC domains (E12)e0
- Interactor
- Pseudomonas syringae DC3000Pseudomonas syringae pv. tomato str. DC3000e0pathogen
- Gene
- RPP1Ae0
- Outcome
- broad-spectrum resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of the RPP1A TIR-NB-ARC domains (E12) using the 35S promoter leads to broad-spectrum resistance to virulent strains of H. parasitica and Pseudomonas syringae DC3000”
e1“stable transgenic lines of Arabidopsis”
Claim · model-written summary
TIR-NB-ARC immunity depends on the salicylic acid resistance pathway
- Taxon
- ArabidopsisArabidopsise1
- Compound
- salicylic acide0
- Outcome
- constitutive immunitye0
- Qualifier
- activation of the salicylic acid-dependent resistance pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TIR-NB-ARC-mediated constitutive immunity is due to activation of the salicylic acid-dependent resistance pathway”
e1“stable transgenic lines of Arabidopsis”
Claim · model-written summary
An EDS1 mutation relieved constitutive immunity
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- mutation in EDS1e0
- Gene
- EDS1e0
- Outcome
- constitutive immunitye1
- Direction
- relievede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is relieved by either a mutation in EDS1 or the presence of the salicylate hydroxylase gene, NahG”
e1“The TIR-NB-ARC-mediated constitutive immunity”
e2“stable transgenic lines of Arabidopsis”
Claim · model-written summary
NahG relieved constitutive immunity
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- presence of the salicylate hydroxylase gene, NahGe0
- Gene
- NahGe0
- Outcome
- constitutive immunitye1
- Direction
- relievede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is relieved by either a mutation in EDS1 or the presence of the salicylate hydroxylase gene, NahG”
e1“The TIR-NB-ARC-mediated constitutive immunity”
e2“stable transgenic lines of Arabidopsis”
Claim · model-written summary
35S::E12 plants had reduced growth
- Taxon
- ArabidopsisArabidopsise1
- Material
- 35S::E12e0
- Outcome
- Growthe0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth of 35S::E12 plants is reduced”
e1“stable transgenic lines of Arabidopsis”
Claim · model-written summary
A hydrophobic N-terminal peptide directs RPP1A into membranes
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- RPP1Ae0
- Outcome
- RPP1A protein into membranese0
- Qualifier
- hydrophobic peptide at its N-terminuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RPP1A carries a hydrophobic peptide at its N-terminus that directs the RPP1A protein into membranes”
e1“Arabidopsis thaliana”
Claim · model-written summary
RPP1A resides in the endoplasmic reticulum or Golgi apparatus
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- RPP1Ae0
- Outcome
- endoplasmic reticulum and/or Golgi apparatuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RPP1A resides in the endoplasmic reticulum and/or Golgi apparatus”
e1“Arabidopsis thaliana”
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See all 147 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.